Puromycin
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MedKoo CAT#: 571550

CAS#: 53-79-2

Description: Puromycin is an antibiotic protein synthesis inhibitor which causes premature chain termination during translation. It is a cinnamamido ADENOSINE found in STREPTOMYCES alboniger. It inhibits protein synthesis by binding to RNA. It is an antineoplastic and antitrypanosomal agent and is used in research as an inhibitor of protein synthesis. It also serves an an anticancer drug by supressing the growth and metastasis in esophagus cancer cells by Akt phosphorylation.

Chemical Structure

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Puromycin
CAS# 53-79-2
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 571550
Name: Puromycin
CAS#: 53-79-2
Chemical Formula: C22H29N7O5
Exact Mass: 471.22
Molecular Weight: 471.520
Elemental Analysis: C, 56.04; H, 6.20; N, 20.79; O, 16.97

Price and Availability

Size Price Availability Quantity
50mg USD 450 2 Weeks
100mg USD 750 2 Weeks
200mg USD 1250 2 Weeks
500mg USD 2650 2 Weeks
1g USD 3850 2 Weeks
2g USD 6450 2 Weeks
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Synonym: Puromycin; Puromycine; NSC-3055; NSC3055; NSC 3055

IUPAC/Chemical Name: Adenosine, 3'-(((2S)-2-amino-3-(4-methoxyphenyl)-1-oxopropyl)amino)-3'-deoxy-N,N-dimethyl-

InChi Key: RXWNCPJZOCPEPQ-NVWDDTSBSA-N

InChi Code: InChI=1S/C22H29N7O5/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32)/t14-,15+,16+,18+,22+/m0/s1

SMILES Code: OC[C@@H]1[C@@H](NC([C@@H](N)CC2=CC=C(OC)C=C2)=O)[C@H]([C@H](N3C=NC4=C(N=CN=C43)N(C)C)O1)O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Product Data:
Product Data
Instruction:
View handling instruction
Biological target: Puromycin inhibits protein synthesis by binding to RNA. It is an antineoplastic and antitrypanosomal agent and is used in research as an inhibitor of protein synthesis. It also serves an an anticancer drug by suppressing the growth and metastasis in esophagus cancer cells by Akt phosphorylation.
In vitro activity: The effects of Puromycin aminonucleoside (PAN) were investigated on podocytes in the context of diabetic nephropathy. PAN treatment had a dose- and time-dependent inhibitory effect on podocyte proliferation, suggesting its role in suppressing cell growth. KLF5 overexpression induced cell cycle arrest in podocytes when regulated by PAN. KLF5 overexpression was shown to inhibit PAN-induced apoptosis of podocyte. Reference: Mol Med Rep. 2018 Oct;18(4):3843-3849. https://pubmed.ncbi.nlm.nih.gov/30106142/
In vivo activity: In adult zebrafish, puromycin administration led to kidney injury, causing rapid clearance of substances from the kidney and resulting in morphological changes in the kidney structure, which recovered over time. During the process of nephron injury and regeneration, various molecular markers associated with kidney injury and repair showed significant changes, including increased expression of renal progenitor marker lhx1a and upregulation of NFκB, TNFα, Nampt, and p-ERK. Reference: Anim Cells Syst (Seoul). 2023 Apr 20;27(1):112-119. https://pubmed.ncbi.nlm.nih.gov/37089626/

Preparing Stock Solutions

The following data is based on the product molecular weight 471.520000000000000000000000000000 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Li Y, Sui X, Hu X, Hu Z. Overexpression of KLF5 inhibits puromycin‑induced apoptosis of podocytes. Mol Med Rep. 2018 Oct;18(4):3843-3849. doi: 10.3892/mmr.2018.9366. Epub 2018 Aug 9. PMID: 30106142; PMCID: PMC6131625. 2. Hernández-Damián J, Tecalco-Cruz AC, Ríos-López DG, Vázquez-Victorio G, Vázquez-Macías A, Caligaris C, Sosa-Garrocho M, Flores-Pérez B, Romero-Avila M, Macías-Silva M. Downregulation of SnoN oncoprotein induced by antibiotics anisomycin and puromycin positively regulates transforming growth factor-β signals. Biochim Biophys Acta. 2013 Nov;1830(11):5049-58. doi: 10.1016/j.bbagen.2013.07.006. Epub 2013 Jul 18. PMID: 23872350. 3. Koun S, Park HJ, Jung SM, Cha JJ, Cha DR, Kang YS. Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish. Anim Cells Syst (Seoul). 2023 Apr 20;27(1):112-119. doi: 10.1080/19768354.2023.2203211. PMID: 37089626; PMCID: PMC10120544. 4. Sakamoto H, Hirakawa Y, Ishida KI, Keeling PJ, Kita K, Matsuzaki M. Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol Int. 2019 Apr;69:13-16. doi: 10.1016/j.parint.2018.10.011. Epub 2018 Oct 30. PMID: 30389616.
In vitro protocol: 1. Li Y, Sui X, Hu X, Hu Z. Overexpression of KLF5 inhibits puromycin‑induced apoptosis of podocytes. Mol Med Rep. 2018 Oct;18(4):3843-3849. doi: 10.3892/mmr.2018.9366. Epub 2018 Aug 9. PMID: 30106142; PMCID: PMC6131625. 2. Hernández-Damián J, Tecalco-Cruz AC, Ríos-López DG, Vázquez-Victorio G, Vázquez-Macías A, Caligaris C, Sosa-Garrocho M, Flores-Pérez B, Romero-Avila M, Macías-Silva M. Downregulation of SnoN oncoprotein induced by antibiotics anisomycin and puromycin positively regulates transforming growth factor-β signals. Biochim Biophys Acta. 2013 Nov;1830(11):5049-58. doi: 10.1016/j.bbagen.2013.07.006. Epub 2013 Jul 18. PMID: 23872350.
In vivo protocol: 1. Koun S, Park HJ, Jung SM, Cha JJ, Cha DR, Kang YS. Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish. Anim Cells Syst (Seoul). 2023 Apr 20;27(1):112-119. doi: 10.1080/19768354.2023.2203211. PMID: 37089626; PMCID: PMC10120544. 2. Sakamoto H, Hirakawa Y, Ishida KI, Keeling PJ, Kita K, Matsuzaki M. Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol Int. 2019 Apr;69:13-16. doi: 10.1016/j.parint.2018.10.011. Epub 2018 Oct 30. PMID: 30389616.

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1: Guo X, Duan Y, Ye X, Hu L, Xu T, Tong L, Yu M. Stable silencing of dll4 gene suppresses the growth and metastasis of esophagus cancer cells by attenuating Akt phosphorylation. Oncol Rep. 2018 May 8. doi: 10.3892/or.2018.6427. [Epub ahead of print] PubMed PMID: 29749499. 2: Yik-Sham Chung C, Timblin GA, Saijo K, Chang CJ. Versatile Histochemical Approach to Detection of Hydrogen Peroxide in Cells and Tissues Based on Puromycin Staining. J Am Chem Soc. 2018 May 3. doi: 10.1021/jacs.8b02279. [Epub ahead of print] PubMed PMID: 29722974. 3: Ahmad F, Salahuddin M, Alsamman K, Herzallah HK, Al-Otaibi ST. Neonatal maternal deprivation impairs localized de novo activity-induced protein translation at the synapse in the rat hippocampus. Biosci Rep. 2018 Apr 26. pii: BSR20180118. doi: 10.1042/BSR20180118. [Epub ahead of print] PubMed PMID: 29700212. 4: Argüello RJ, Simoes MR, Mendes A, Camosseto V, Torres AG, de Pouplana LR, van de Pavert SA, Gatti E, Pierre P. SunRiSE: measuring translation elongation at single cell resolution by flow cytometry. J Cell Sci. 2018 Apr 26. pii: jcs.214346. doi: 10.1242/jcs.214346. [Epub ahead of print] PubMed PMID: 29700204. 5: Wu L, Blum W, Zhu CQ, Yun Z, Pecze L, Kohno M, Chan ML, Zhao Y, Felley-Bosco E, Schwaller B, de Perrot M. Putative cancer stem cells may be the key target to inhibit cancer cell repopulation between the intervals of chemoradiation in murine mesothelioma. BMC Cancer. 2018 Apr 27;18(1):471. doi: 10.1186/s12885-018-4354-1. PubMed PMID: 29699510; PubMed Central PMCID: PMC5921988. 6: Lyu C, Shen J, Wang R, Gu H, Zhang J, Xue F, Liu X, Liu W, Fu R, Zhang L, Li H, Zhang X, Cheng T, Yang R, Zhang L. Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system. Stem Cell Res Ther. 2018 Apr 6;9(1):92. doi: 10.1186/s13287-018-0839-8. PubMed PMID: 29625575; PubMed Central PMCID: PMC5889534. 7: Stefanik MT, Milovanovic M, Werner CT, Spainhour JCG, Wolf ME. Withdrawal From Cocaine Self-administration Alters the Regulation of Protein Translation in the Nucleus Accumbens. Biol Psychiatry. 2018 Feb 23. pii: S0006-3223(18)30113-6. doi: 10.1016/j.biopsych.2018.02.012. [Epub ahead of print] PubMed PMID: 29622268. 8: Gong J, Jin J, Zhao L, Li Y, Li Y, He Q. Tripterygium glycoside protects against puromycin amino nucleoside induced podocyte injury by upregulating autophagy. Int J Mol Med. 2018 Jul;42(1):115-122. doi: 10.3892/ijmm.2018.3598. Epub 2018 Mar 29. PubMed PMID: 29620171. 9: Ren H, Hu X, Liu Y, Dai D, Liu X, Wang Z, Yang Y, Li X, Liu Y, Tang R. Salvia przewalskii extract of total phenolic acids inhibit TLR4 signaling activation in podocyte injury induced by puromycin aminonucleoside in vitro. Ren Fail. 2018 Nov;40(1):273-279. doi: 10.1080/0886022X.2018.1456460. PubMed PMID: 29619899. 10: Watanabe S, Sakurai T, Nakamura S, Miyoshi K, Sato M. The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones. Int J Mol Sci. 2018 Apr 4;19(4). pii: E1075. doi: 10.3390/ijms19041075. PubMed PMID: 29617297. 11: Cátia FC, Janete QS, Benedita SM, Liliana SS, Isabel SS, Roberto RA Jr., Manuel P. Calcitriol Prevents Cardiovascular Repercussions in Puromycin Aminonucleoside-Induced Nephrotic Syndrome. Biomed Res Int. 2018 Jan 23;2018:3609645. doi: 10.1155/2018/3609645. eCollection 2018. PubMed PMID: 29607318; PubMed Central PMCID: PMC5828333. 12: Dijk FJ, van Dijk M, Walrand S, van Loon LJC, van Norren K, Luiking YC. Differential effects of leucine and leucine-enriched whey protein on skeletal muscle protein synthesis in aged mice. Clin Nutr ESPEN. 2018 Apr;24:127-133. doi: 10.1016/j.clnesp.2017.12.013. Epub 2018 Jan 11. PubMed PMID: 29576350. 13: Yamanishi A, Matsuba A, Kondo R, Akamatsu R, Tanaka S, Tokunaga M, Horie K, Kokubu C, Ishida Y, Takeda J. Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis. Nucleic Acids Res. 2018 Mar 15. doi: 10.1093/nar/gky183. [Epub ahead of print] PubMed PMID: 29554276. 14: Bastide A, Yewdell JW, David A. The RiboPuromycylation Method (RPM): an Immunofluorescence Technique to Map Translation Sites at the Sub-cellular Level. Bio Protoc. 2018 Jan 5;8(1). pii: e2669. doi: 10.21769/BioProtoc.2669. PubMed PMID: 29552591; PubMed Central PMCID: PMC5856242. 15: Liu T, Tian DW, Zou LJ, Liu FY, Can QY, Yang JK, Xu JP, Huang XW, Xi JQ, Zhu ML, Mo MH, Zhang KQ. Quantitative proteomics revealed partial fungistatic mechanism of ammonia against conidial germination of nematode-trapping fungus Arthrobotrys oligospora ATCC24927. Int J Biochem Cell Biol. 2018 May;98:104-112. doi: 10.1016/j.biocel.2018.03.009. Epub 2018 Mar 12. PubMed PMID: 29544894. 16: Guo WH, Chen ZY, Chen H, Lin T, Zhao ML, Liu H, Yu J, Hu YF, Li GX. [Sericin regulates proliferation of human gastric cancer MKN45 cells through autophagic pathway]. Nan Fang Yi Ke Da Xue Xue Bao. 2018 Feb 20;38(2):148-154. Chinese. PubMed PMID: 29502052. 17: Toffoli B, Zennaro C, Winkler C, Giordano Attianese GMP, Bernardi S, Carraro M, Gilardi F, Desvergne B. Hemicentin 1 influences podocyte dynamic changes in glomerular diseases. Am J Physiol Renal Physiol. 2018 Feb 28. doi: 10.1152/ajprenal.00198.2017. [Epub ahead of print] PubMed PMID: 29488390. 18: Ai J, Sun JH, Wan T, Ma J, Feng L, Yao K. Generation of an anti-angiogenic endothelial progenitor cell line via endostatin gene transfer. Mol Med Rep. 2018 Apr;17(4):5814-5820. doi: 10.3892/mmr.2018.8623. Epub 2018 Feb 21. PubMed PMID: 29484399; PubMed Central PMCID: PMC5866025. 19: Rider SA, Bruton FA, Collins RG, Conway BR, Mullins JJ. The Efficacy of Puromycin and Adriamycin for Induction of Glomerular Failure in Larval Zebrafish Validated by an Assay of Glomerular Permeability Dynamics. Zebrafish. 2018 Feb 26. doi: 10.1089/zeb.2017.1527. [Epub ahead of print] PubMed PMID: 29480793. 20: Forester CM, Zhao Q, Phillips NJ, Urisman A, Chalkley RJ, Oses-Prieto JA, Zhang L, Ruggero D, Burlingame AL. Revealing nascent proteomics in signaling pathways and cell differentiation. Proc Natl Acad Sci U S A. 2018 Mar 6;115(10):2353-2358. doi: 10.1073/pnas.1707514115. Epub 2018 Feb 21. PubMed PMID: 29467287; PubMed Central PMCID: PMC5877968.