SATA Protein Modifier
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MedKoo CAT#: 571642

CAS#: 76931-93-6

Description: SATA Protein Modifier, or N-Succinimidyl S-Acetylthioacetate, is a protein modification reagent used to convert primary amine groups into protected sulfhydryls (thiol; -SH) for later reaction with sulfhydryl reactive crosslinkers such as Sulfo-SMCC, Sulfo-MBS, etc.


Chemical Structure

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SATA Protein Modifier
CAS# 76931-93-6

Theoretical Analysis

MedKoo Cat#: 571642
Name: SATA Protein Modifier
CAS#: 76931-93-6
Chemical Formula: C8H9NO4S
Exact Mass: 215.0252
Molecular Weight: 215.22
Elemental Analysis: C, 44.65; H, 4.22; N, 6.51; O, 29.73; S, 14.90

Price and Availability

Size Price Availability Quantity
100.0mg USD 260.0 2 Weeks
1.0g USD 990.0 2 Weeks
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Synonym: SATA Protein Modifier

IUPAC/Chemical Name: N-Succinimidyl S-Acetylthioacetate

InChi Key: BBCGXZULVWBCGP-UHFFFAOYSA-N

InChi Code: InChI=1S/C8H9NO4S/c1-5(10)14-8(13)4-9-6(11)2-3-7(9)12/h2-4H2,1H3

SMILES Code: O=C(SC(C)=O)CN1C(CCC1=O)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 215.22 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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1: Lin JT, Chen PC, Goud TV, Huang BR, Lin TC, Biellmann JF, Chen CS. A sulfhydryl-reactive ruthenium (II) complex and its conjugation to protein G as a universal reagent for fluorescent immunoassays. PLoS One. 2012;7(4):e36086. doi: 10.1371/journal.pone.0036086. Epub 2012 Apr 26. PubMed PMID: 22563441; PubMed Central PMCID: PMC3338566.

2: Genç R, Murphy D, Fragoso A, Ortiz M, O'Sullivan CK. Signal-enhancing thermosensitive liposomes for highly sensitive immunosensor development. Anal Chem. 2011 Jan 15;83(2):563-70. doi: 10.1021/ac1023765. Epub 2010 Dec 14. PubMed PMID: 21155541.

3: Vera-Robles LI, Escobar-Alarcón L, Picquart M, Hernández-Pozos JL, Haro-Poniatowski E. A Biological Approach for the Synthesis of Bismuth Nanoparticles: Using Thiolated M13 Phage as Scaffold. Langmuir. 2016 Apr 5;32(13):3199-206. doi: 10.1021/acs.langmuir.5b04369. Epub 2016 Mar 24. PubMed PMID: 27010536.



Additional Information

The resulting sulfhydryl is available for use in subsequent reactions after exposure to Hydroxylamine. After this deprotection step is performed to create the thioacetylated peptide, the free sulfhydryl containing peptide can then be conjugated to make a hapten-carrier conjugate, polymer, etc.