NHS-Biotin
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MedKoo CAT#: 571634

CAS#: 35013-72-0

Description: NHS-Biotin, Succinimidobiotin, is a primary amine reactive biotinylation reagent used in Biotin-Streptavidin binding reactions. The NHS-Biotin molecule is cell membrane permeable and can be used to covalently label intracellular proteins.

Chemical Structure

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NHS-Biotin
CAS# 35013-72-0
Instruction

Theoretical Analysis

MedKoo Cat#: 571634
Name: NHS-Biotin
CAS#: 35013-72-0
Chemical Formula: C14H19N3O5S
Exact Mass: 341.10
Molecular Weight: 341.380
Elemental Analysis: C, 49.26; H, 5.61; N, 12.31; O, 23.43; S, 9.39

Price and Availability

Size Price Availability Quantity
100mg USD 255
1g USD 900
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Synonym: NHS-Biotin, Succinimidobiotin, Biotin-OSU; Biotin-NHS ester

IUPAC/Chemical Name: Biotin N-hydroxysuccinimide ester

InChi Key: YMXHPSHLTSZXKH-RVBZMBCESA-N

InChi Code: InChI=1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1

SMILES Code: O=C1N[C@@]([H])([C@]([H])(CS2)N1)[C@@H]2CCCCC(ON3C(CCC3=O)=O)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info: The water soluble version of NHS-Biotin is Sulfo-NHS-Biotin.

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 341.38 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

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1: Gabant G, Augier J, Armengaud J. Assessment of solvent residues accessibility using three Sulfo-NHS-biotin reagents in parallel: application to footprint changes of a methyltransferase upon binding its substrate. J Mass Spectrom. 2008 Mar;43(3):360-70. PubMed PMID: 17968972.

2: Pintado CO, Moreno A, Barbancho M, Pérez de la Lastra JM, Llanes D. The beta chain of the GPIIb molecule on ruminant leukocytes and platelets is not labelled by the sulfo-NHS-biotin method. Vox Sang. 1995;69(3):248-9. PubMed PMID: 8578739.