Sulfo-MBS Crosslinker
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 571614

CAS#: 92921-25-0

Description: Sulfo-MBS Crosslinker, also known as m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester, is a non-cleavable, water soluble, heterobifunctional protein crosslinking reagent that is commonly used to crosslink haptens to carrier proteins and enzymes to antibody.

Chemical Structure

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Sulfo-MBS Crosslinker
CAS# 92921-25-0
Instruction

Theoretical Analysis

MedKoo Cat#: 571614
Name: Sulfo-MBS Crosslinker
CAS#: 92921-25-0
Chemical Formula: C15H10N2O9S
Exact Mass: 394.01
Molecular Weight: 394.310
Elemental Analysis: C, 45.69; H, 2.56; N, 7.10; O, 36.52; S, 8.13

Price and Availability

Size Price Availability Quantity
100mg USD 330
1g USD 1450
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Synonym: Sulfo-MBS Crosslinker, Sulfo-MBS Cross linker, Sulfo-MBS Cross-linker

IUPAC/Chemical Name: 3-Maleimidobenzoic acid N-hydroxysulfosuccinimide ester

InChi Key: DIYPCWKHSODVAP-UHFFFAOYSA-N

InChi Code: InChI=1S/C15H10N2O9S/c18-11-4-5-12(19)16(11)9-3-1-2-8(6-9)15(22)26-17-13(20)7-10(14(17)21)27(23,24)25/h1-6,10H,7H2,(H,23,24,25)

SMILES Code: O=C(ON1C(C(S(=O)(O)=O)CC1=O)=O)C2=CC=CC(N3C(C=CC3=O)=O)=C2

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info: The Sulfo-MBS crosslinking reagent's NHS ester and maleimide reactive groups react with amine and sulfhydryl moites, respectively. SATA Protein Modifier can be used to add sulfhydryl groups (thiols, -SH) to proteins or peptides prior to Sulfo-MBS crosslinking.

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 394.31 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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1: Chen F, Nielsen S, Zenobi R. Understanding chemical reactivity for homo- and heterobifunctional protein cross-linking agents. J Mass Spectrom. 2013 Jul;48(7):807-12. doi: 10.1002/jms.3224. PubMed PMID: 23832936.

2: Nadeau OW, Carlson GM. Protein Interactions Captured by Chemical Cross-linking: Simple Cross-linking Screen Using Sulfo-MBS. CSH Protoc. 2007 Apr 1;2007:pdb.prot4587. doi: 10.1101/pdb.prot4587. PubMed PMID: 21357052.

3: Kühn-Hölsken E, Lenz C, Dickmanns A, Hsiao HH, Richter FM, Kastner B, Ficner R, Urlaub H. Mapping the binding site of snurportin 1 on native U1 snRNP by cross-linking and mass spectrometry. Nucleic Acids Res. 2010 Sep;38(16):5581-93. doi: 10.1093/nar/gkq272. Epub 2010 Apr 26. PubMed PMID: 20421206; PubMed Central PMCID: PMC2938196.

4: Söllner T, Rassow J, Wiedmann M, Schlossmann J, Keil P, Neupert W, Pfanner N. Mapping of the protein import machinery in the mitochondrial outer membrane by crosslinking of translocation intermediates. Nature. 1992 Jan 2;355(6355):84-7. PubMed PMID: 1530986.