PS-48
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 562562

CAS#: 1180676-32-7

Description: PS-48 is a phosphoinositide-dependent protein kinase-1 (PDK1) activator.


Chemical Structure

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PS-48
CAS# 1180676-32-7

Theoretical Analysis

MedKoo Cat#: 562562
Name: PS-48
CAS#: 1180676-32-7
Chemical Formula: C17H15ClO2
Exact Mass: 286.08
Molecular Weight: 286.750
Elemental Analysis: C, 71.21; H, 5.27; Cl, 12.36; O, 11.16

Price and Availability

Size Price Availability Quantity
50mg USD 550 2 Weeks
100mg USD 950 2 Weeks
200mg USD 1650 2 Weeks
500mg USD 2650 2 Weeks
1g USD 3250 2 Weeks
2g USD 5650 2 Weeks
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Synonym: PS-48; PS 48; PS48;

IUPAC/Chemical Name: (2Z)-5-(4-Chlorophenyl)-3-phenyl-2-pentenoic acid

InChi Key: LLJYFDRQFPQGNY-QINSGFPZSA-N

InChi Code: InChI=1S/C17H15ClO2/c18-16-10-7-13(8-11-16)6-9-15(12-17(19)20)14-4-2-1-3-5-14/h1-5,7-8,10-12H,6,9H2,(H,19,20)/b15-12-

SMILES Code: O=C(O)/C=C(C1=CC=CC=C1)/CCC2=CC=C(Cl)C=C2

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO and ethanol

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Biological target: PS-48 is a PDK1 activator and binds to the HM/PIF binding pocket rather than the ATP-binding site.
In vitro activity: The use of 10 µM PS-48 significantly increased the proportion of porcine oocytes reaching the metaphase II (MII) stage and improved cumulus cell expansion. The activation of the PI3K/Akt pathway was associated with the regulation of maturation-related genes and proteins, including Akt, cyclin B1, MOS, BMP15, GDF9, CDC2, mTOR, BAX, BCL2, and caspase-3. Reference: Reprod Domest Anim. 2020 Dec;55(12):1678-1687. https://pubmed.ncbi.nlm.nih.gov/32946622/
In vivo activity: In a mouse model for Alzheimer's disease, the oral administration of PS-48 was tested to assess its potential in preventing the decline in learning and memory. PS-48 had a positive impact on spatial learning and memory, especially in the mice on a high-fat die. PS-48 modestly reduced body weight and improved glucose tolerance. Reference: Behav Brain Res. 2023 Feb 13;438:114183. https://pubmed.ncbi.nlm.nih.gov/36404570/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 57.0 198.78
Ethanol 57.0 198.78

Preparing Stock Solutions

The following data is based on the product molecular weight 286.75 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Jiao Y, Zhu S, Li J, Jam Zaheer A, Li M, Huang B. PS48 promotes in vitro maturation and developmental competence of porcine oocytes through activating PI3K/Akt signalling pathway. Reprod Domest Anim. 2020 Dec;55(12):1678-1687. doi: 10.1111/rda.13818. Epub 2020 Nov 10. PMID: 32946622. 2. Mordhorst BR, Murphy SL, Ross RM, Benne JA, Samuel MS, Cecil RF, Redel BK, Spate LD, Murphy CN, Wells KD, Green JA, Prather RS. Pharmacologic treatment of donor cells induced to have a Warburg effect-like metabolism does not alter embryonic development in vitro or survival during early gestation when used in somatic cell nuclear transfer in pigs. Mol Reprod Dev. 2018 Apr;85(4):290-302. doi: 10.1002/mrd.22964. Epub 2018 Mar 5. PMID: 29392839; PMCID: PMC5903921. 3. Querfurth H, Slitt A, DiCamillo A, Surles N, DeBoef B, Lee HK. A PDK-1 allosteric agonist improves spatial learning and memory in a βAPP/PS-1 transgenic mouse-high fat diet intervention model of Alzheimer's disease. Behav Brain Res. 2023 Feb 13;438:114183. doi: 10.1016/j.bbr.2022.114183. Epub 2022 Oct 29. PMID: 36404570. 4. Xu WN, Zheng HL, Yang RZ, Jiang LS, Jiang SD. HIF-1α Regulates Glucocorticoid-Induced Osteoporosis Through PDK1/AKT/mTOR Signaling Pathway. Front Endocrinol (Lausanne). 2020 Jan 28;10:922. doi: 10.3389/fendo.2019.00922. PMID: 32047474; PMCID: PMC6997475.
In vitro protocol: 1. Jiao Y, Zhu S, Li J, Jam Zaheer A, Li M, Huang B. PS48 promotes in vitro maturation and developmental competence of porcine oocytes through activating PI3K/Akt signalling pathway. Reprod Domest Anim. 2020 Dec;55(12):1678-1687. doi: 10.1111/rda.13818. Epub 2020 Nov 10. PMID: 32946622. 2. Mordhorst BR, Murphy SL, Ross RM, Benne JA, Samuel MS, Cecil RF, Redel BK, Spate LD, Murphy CN, Wells KD, Green JA, Prather RS. Pharmacologic treatment of donor cells induced to have a Warburg effect-like metabolism does not alter embryonic development in vitro or survival during early gestation when used in somatic cell nuclear transfer in pigs. Mol Reprod Dev. 2018 Apr;85(4):290-302. doi: 10.1002/mrd.22964. Epub 2018 Mar 5. PMID: 29392839; PMCID: PMC5903921.
In vivo protocol: 1. Querfurth H, Slitt A, DiCamillo A, Surles N, DeBoef B, Lee HK. A PDK-1 allosteric agonist improves spatial learning and memory in a βAPP/PS-1 transgenic mouse-high fat diet intervention model of Alzheimer's disease. Behav Brain Res. 2023 Feb 13;438:114183. doi: 10.1016/j.bbr.2022.114183. Epub 2022 Oct 29. PMID: 36404570. 2. Xu WN, Zheng HL, Yang RZ, Jiang LS, Jiang SD. HIF-1α Regulates Glucocorticoid-Induced Osteoporosis Through PDK1/AKT/mTOR Signaling Pathway. Front Endocrinol (Lausanne). 2020 Jan 28;10:922. doi: 10.3389/fendo.2019.00922. PMID: 32047474; PMCID: PMC6997475.

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1: Mordhorst BR, Murphy SL, Ross RM, Samuel MS, Salazar SR, Ji T, Behura SK, Wells KD, Green JA, Prather RS. Pharmacologic Reprogramming Designed to Induce a Warburg Effect in Porcine Fetal Fibroblasts Alters Gene Expression and Quantities of Metabolites from Conditioned Media Without Increased Cell Proliferation. Cell Reprogram. 2018 Feb;20(1):38-48. doi: 10.1089/cell.2017.0040. PubMed PMID: 29412741; PubMed Central PMCID: PMC5804098.

2: Mordhorst BR, Murphy SL, Ross RM, Benne JA, Samuel MS, Cecil RF, Redel BK, Spate LD, Murphy CN, Wells KD, Green JA, Prather RS. Pharmacologic treatment of donor cells induced to have a Warburg effect-like metabolism does not alter embryonic development in vitro or survival during early gestation when used in somatic cell nuclear transfer in pigs. Mol Reprod Dev. 2018 Apr;85(4):290-302. doi: 10.1002/mrd.22964. Epub 2018 Mar 5. PubMed PMID: 29392839; PubMed Central PMCID: PMC5903921.

3: Han F, Xue M, Chang Y, Li X, Yang Y, Sun B, Chen L. Triptolide Suppresses Glomerular Mesangial Cell Proliferation in Diabetic Nephropathy Is Associated with Inhibition of PDK1/Akt/mTOR Pathway. Int J Biol Sci. 2017 Sep 21;13(10):1266-1275. doi: 10.7150/ijbs.20485. eCollection 2017. PubMed PMID: 29104493; PubMed Central PMCID: PMC5666525.

4: Liu W, Mao J, Xue Y, Zhao Z, Zhang H, Ji X. Nanoparticle Loading Induced Morphological Transitions and Size Fractionation of Coassemblies from PS-b-PAA with Quantum Dots. Langmuir. 2016 Aug 2;32(30):7596-605. doi: 10.1021/acs.langmuir.6b02202. Epub 2016 Jul 22. PubMed PMID: 27447738.

5: Watanabe S, Matsumoto T, Oda M, Yamada K, Takagi J, Taguchi K, Kobayashi T. Insulin augments serotonin-induced contraction via activation of the IR/PI3K/PDK1 pathway in the rat carotid artery. Pflugers Arch. 2016 Apr;468(4):667-77. doi: 10.1007/s00424-015-1759-4. Epub 2015 Nov 17. PubMed PMID: 26577585.

6: Spate LD, Brown A, Redel BK, Whitworth KM, Prather RS. PS48 can replace bovine serum albumin in pig embryo culture medium, and improve in vitro embryo development by phosphorylating AKT. Mol Reprod Dev. 2015 Apr;82(4):315-20. doi: 10.1002/mrd.22474. Epub 2015 Mar 16. PubMed PMID: 25776657; PubMed Central PMCID: PMC4720971.

7: Liu Z, Ren J, Cao J, He J, Yao X, Jin C, Xue Y. Systematic analysis of the Plk-mediated phosphoregulation in eukaryotes. Brief Bioinform. 2013 May;14(3):344-60. doi: 10.1093/bib/bbs041. Epub 2012 Jul 31. Review. PubMed PMID: 22851512.

8: Hussain H, Tan BH, Gudipati CS, He CB, Liu Y, Davis TP. Micelle formation of amphiphilic polystyrene-b-poly(N-vinylpyrrolidone) diblock copolymer in methanol and water-methanol binary mixtures. Langmuir. 2009 May 19;25(10):5557-64. doi: 10.1021/la8042389. PubMed PMID: 19371048.

9: Anthony TG, Polley SD, Vogler AP, Conway DJ. Evidence of non-neutral polymorphism in Plasmodium falciparum gamete surface protein genes Pfs47 and Pfs48/45. Mol Biochem Parasitol. 2007 Dec;156(2):117-23. Epub 2007 Jul 20. PubMed PMID: 17826852.