Pyronine B

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 341323

CAS#: 2150-48-3

Description: Pyronine B is a biochemical.

Chemical Structure

Pyronine B
CAS# 2150-48-3

Theoretical Analysis

MedKoo Cat#: 341323
Name: Pyronine B
CAS#: 2150-48-3
Chemical Formula: C21H27ClN2O
Exact Mass: 358.18
Molecular Weight: 358.910
Elemental Analysis: C, 70.28; H, 7.58; Cl, 9.88; N, 7.81; O, 4.46

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: Pyronine B; NSC 44690; NSC-44690; NSC44690.

IUPAC/Chemical Name: 3,6-Bis(diethylamino)xanthylium chloride


InChi Code: InChI=1S/C21H27N2O.ClH/c1-5-22(6-2)18-11-9-16-13-17-10-12-19(23(7-3)8-4)15-21(17)24-20(16)14-18;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1

SMILES Code: CCN(C1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)C=C2C=C1)CC.[Cl-]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 358.91 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Zhang XF, Zhang J, Lu X. The Fluorescence Properties of Three Rhodamine Dye Analogues: Acridine Red, Pyronin Y and Pyronin B. J Fluoresc. 2015 Jul;25(4):1151-8. doi: 10.1007/s10895-015-1610-5. Epub 2015 Jul 11. PubMed PMID: 26162989.

2: Geng H, Zhang XF. Spectroscopic insights on selfassembly and excited state interactions between rhodamine and phthalocyanine molecules. Spectrochim Acta A Mol Biomol Spectrosc. 2015 Mar 15;139:13-9. doi: 10.1016/j.saa.2014.12.010. Epub 2014 Dec 16. PubMed PMID: 25546492.

3: Li Y, To J, Verdià-Baguena C, Dossena S, Surya W, Huang M, Paulmichl M, Liu DX, Aguilella VM, Torres J. Inhibition of the human respiratory syncytial virus small hydrophobic protein and structural variations in a bicelle environment. J Virol. 2014 Oct;88(20):11899-914. doi: 10.1128/JVI.00839-14. Epub 2014 Aug 6. PubMed PMID: 25100835; PubMed Central PMCID: PMC4178740.

4: Chen WX, Zhu HL, Xue M, Zhou H, Zhao F, Yan N, Chen Y. Quick staining technique for myeloperoxidase using potassium iodide and oxidized pyronine B. Acta Histochem. 2014 Jan;116(1):292-6. doi: 10.1016/j.acthis.2013.08.001. Epub 2013 Sep 14. PubMed PMID: 24044784.

5: Ulusoy Hİ, Yılmaz O, Gürkan R. A micellar improved method for trace levels selenium quantification in food samples, alcoholic and nonalcoholic beverages through CPE/FAAS. Food Chem. 2013 Aug 15;139(1-4):1008-14. doi: 10.1016/j.foodchem.2013.01.087. Epub 2013 Feb 8. PubMed PMID: 23561203.

6: Baig JA, Kazi TG, Arain MB, Afridi HI, Mahar KP. Quantification of arsenic in dialysate solution and scalp hair samples of kidney failure patients by cloud point extraction and electrothermal atomic absorption spectroscopy. J AOAC Int. 2012 Nov-Dec;95(6):1755-60. PubMed PMID: 23451395.

7: Gür B, Meral K. The effect of poly(vinyl alcohol) on the photophysical properties of pyronin dyes in aqueous solution: a spectroscopic study. Spectrochim Acta A Mol Biomol Spectrosc. 2013 Jan 15;101:306-13. doi: 10.1016/j.saa.2012.09.098. Epub 2012 Oct 12. PubMed PMID: 23123237.

8: Ulusoy Hİ, Akçay M, Ulusoy S, Gürkan R. Determination of ultra trace arsenic species in water samples by hydride generation atomic absorption spectrometry after cloud point extraction. Anal Chim Acta. 2011 Oct 10;703(2):137-44. doi: 10.1016/j.aca.2011.07.026. Epub 2011 Jul 23. PubMed PMID: 21889627.

9: Ulusoy Hİ, Akçay M, Gürkan R. Development of an inexpensive and sensitive method for the determination of low quantity of arsenic species in water samples by CPE-FAAS. Talanta. 2011 Sep 15;85(3):1585-91. doi: 10.1016/j.talanta.2011.06.053. Epub 2011 Jun 24. PubMed PMID: 21807225.

10: Ni Y, Zhu Y, Ma X. A simple solution combustion route for the preparation of metal-doped TiO2 nanoparticles and their photocatalytic degradation properties. Dalton Trans. 2011 Apr 14;40(14):3689-94. doi: 10.1039/c0dt01534b. Epub 2011 Mar 3. PubMed PMID: 21369610.

11: Bordello J, Reija B, Al-Soufi W, Novo M. Host-assisted guest self-assembly: enhancement of the dimerization of pyronines Y and B by gamma-cyclodextrin. Chemphyschem. 2009 Apr 14;10(6):931-9. doi: 10.1002/cphc.200800776. PubMed PMID: 19294686.

12: Li J, Ni Y, Liao K, Hong J. Hydrothermal synthesis of Ni(12)P(5) hollow microspheres, characterization and photocatalytic degradation property. J Colloid Interface Sci. 2009 Apr 1;332(1):231-6. doi: 10.1016/j.jcis.2008.12.058. Epub 2008 Dec 25. PubMed PMID: 19144350.

13: Villagra NA, Hidalgo AA, Santiviago CA, Saavedra CP, Mora GC. SmvA, and not AcrB, is the major efflux pump for acriflavine and related compounds in Salmonella enterica serovar Typhimurium. J Antimicrob Chemother. 2008 Dec;62(6):1273-6. doi: 10.1093/jac/dkn407. Epub 2008 Sep 26. PubMed PMID: 18819967.

14: Chen LH, Yin H, Yang ZX, Zhang KM, Liu LZ, Shen HX. [Fluorescence investigation on interaction between artemisinin (qinghaosu) and hemin and its analytical application]. Guang Pu Xue Yu Guang Pu Fen Xi. 2006 Sep;26(9):1640-3. Chinese. PubMed PMID: 17112036.

15: Rezaei B, Majidi N. A simple and high sensitive spectrophotometric method for ultra trace determination of ruthenium with its catalytic effect on the oxidation of pyronin B by periodate. Spectrochim Acta A Mol Biomol Spectrosc. 2007 Apr;66(4-5):869-73. Epub 2006 May 10. PubMed PMID: 16872868.

16: Reija B, Al-Soufi W, Novo M, Vazquez Tato J. Specific interactions in the inclusion complexes of pyronines Y and B with beta-cyclodextrin. J Phys Chem B. 2005 Feb 3;109(4):1364-70. PubMed PMID: 16851104.

17: Gao F, Li YX, Zhang L, Wang L. Cetyltrimethylammonium bromide sensitized resonance light-scattering of nucleic acid--Pyronine B and its analytical application. Spectrochim Acta A Mol Biomol Spectrosc. 2004 Sep;60(11):2505-9. PubMed PMID: 15294236.

18: Cakar M, Temirci C, Türüt A. Determination of the density distribution of interface states from high- and low-frequency capacitance characteristics of the tin/organic pyronine-B/p-type silicon structure. Chemphyschem. 2002 Aug 16;3(8):701-4. PubMed PMID: 12503152.

19: Paleologos EK, Koupparis MA, Karayannis MI, Veltsistas PG. Nonaqueous catalytic fluorometric trace determination of vanadium based on the pyronine B-hydrogen peroxide reaction and flow injection after cloud point extraction. Anal Chem. 2001 Sep 15;73(18):4428-33. PubMed PMID: 11575789.

20: Diaz G, Diana A, Setzu MD, Sogos V, Zucca A. Nuclear dyes and cytoplasmic staining. Cytometry. 1997 Dec 1;29(4):375-6. PubMed PMID: 9415421.