NG25 free base
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MedKoo CAT#: 562503

CAS#: 1315355-93-1 (free base)

Description: NG-25 is a type II kinase inhibitor that inhibits MAP4K2 and TAK1. It also inhibits the Src family kinases Src and LYN and Abl family kinases as well as CSK, FER, and p38α. NG 25 prevents TNF-α-induced IKKα/β phosphorylation and IκB-α degradation in L929 cells. It inhibits secretion of IFN-α and IFN-β induced by CpG type B and CL097, respectively. NG 25 decreases cell viability of HCT116KRASWT, and to a greater degree of HCT116KRASG13D, colorectal cancer cells in a concentration-dependent manner. It also reduces tumor growth and increases the number of TUNEL-positive tumor cells in a CT26KRASG12D mouse orthotopic model of colorectal cancer.


Chemical Structure

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NG25 free base
CAS# 1315355-93-1 (free base)

Theoretical Analysis

MedKoo Cat#: 562503
Name: NG25 free base
CAS#: 1315355-93-1 (free base)
Chemical Formula: C29H30F3N5O2
Exact Mass: 537.24
Molecular Weight: 537.580
Elemental Analysis: C, 64.79; H, 5.63; F, 10.60; N, 13.03; O, 5.95

Price and Availability

Size Price Availability Quantity
5mg USD 485 2 Weeks
10mg USD 850 2 Weeks
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Related CAS #: 2108554-00-1 (HCl)   1315355-93-1 (free base)    

Synonym: NG25; NG-25; NG 25; NG25 free base;

IUPAC/Chemical Name: N-[4-[(4-Ethyl-1-piperazinyl)methyl]-3-(trifluoromethyl)phenyl]-4-methyl-3-(1H-pyrrolo[2,3-b]pyridin-4-yloxy)-benzamide

InChi Key: SMPGEBOIKULBCT-UHFFFAOYSA-N

InChi Code: InChI=1S/C29H30F3N5O2/c1-3-36-12-14-37(15-13-36)18-21-6-7-22(17-24(21)29(30,31)32)35-28(38)20-5-4-19(2)26(16-20)39-25-9-11-34-27-23(25)8-10-33-27/h4-11,16-17H,3,12-15,18H2,1-2H3,(H,33,34)(H,35,38)

SMILES Code: O=C(NC1=CC=C(CN2CCN(CC)CC2)C(C(F)(F)F)=C1)C3=CC=C(C)C(OC4=C5C(NC=C5)=NC=C4)=C3

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
Soluble in DMSO 0.0 100.00

Preparing Stock Solutions

The following data is based on the product molecular weight 537.58 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Scholey DV, Morgan NK, Riemensperger A, Hardy R, Burton EJ. Effect of supplementation of phytase to diets low in inorganic phosphorus on growth performance and mineralization of broilers. Poult Sci. 2018 Mar 16. doi: 10.3382/ps/pey088. [Epub ahead of print] PubMed PMID: 29562263.

2: Pan G, Lyu T, Mortimer R. Comment: Closing phosphorus cycle from natural waters: re-capturing phosphorus through an integrated water-energy-food strategy. J Environ Sci (China). 2018 Mar;65:375-376. doi: 10.1016/j.jes.2018.02.018. PubMed PMID: 29548409.

3: Simpson SD, Barber I, Winfield IJ. Understanding fish populations. J Fish Biol. 2018 Mar;92(3):563-568. doi: 10.1111/jfb.13574. PubMed PMID: 29537087.

4: Jiang L, Ball G, Hodgman C, Coules A, Zhao H, Lu C. Analysis of Gene Regulatory Networks of Maize in Response to Nitrogen. Genes (Basel). 2018 Mar 8;9(3). pii: E151. doi: 10.3390/genes9030151. PubMed PMID: 29518046; PubMed Central PMCID: PMC5867872.

5: Carter AJ, Hall EJ. Investigating factors affecting the body temperature of dogs competing in cross country (canicross) races in the UK. J Therm Biol. 2018 Feb;72:33-38. doi: 10.1016/j.jtherbio.2017.12.006. Epub 2017 Dec 21. PubMed PMID: 29496012.

6: Bian Z, Yang Q, Li T, Cheng R, Barnett Y, Lu C. Study of the beneficial effects of green light on lettuce grown under short-term continuous red and blue light-emitting diodes. Physiol Plant. 2018 Mar 1. doi: 10.1111/ppl.12713. [Epub ahead of print] PubMed PMID: 29493775.

7: Saidi H, Bérubé J, Laraba-Djebari F, Hammoudi-Triki D. Involvement of Alveolar Macrophages and Neutrophils in Acute Lung Injury After Scorpion Envenomation: New Pharmacological Targets. Inflammation. 2018 Feb 28. doi: 10.1007/s10753-018-0731-9. [Epub ahead of print] PubMed PMID: 29492721.

8: Farnworth MJ, Packer RMA, Sordo L, Chen R, Caney SMA, Gunn-Moore DA. In the Eye of the Beholder: Owner Preferences for Variations in Cats' Appearances with Specific Focus on Skull Morphology. Animals (Basel). 2018 Feb 20;8(2). pii: E30. doi: 10.3390/ani8020030. PubMed PMID: 29461472; PubMed Central PMCID: PMC5836038.

9: Hall HN, Masey O'Neill HV, Scholey D, Burton E, Dickinson M, Fitches EC. Amino acid digestibility of larval meal (Musca domestica) for broiler chickens. Poult Sci. 2018 Apr 1;97(4):1290-1297. doi: 10.3382/ps/pex433. PubMed PMID: 29452386.

10: Lyu T, He K, Dong R, Wu S. The intensified constructed wetlands are promising for treatment of ammonia stripped effluent: Nitrogen transformations and removal pathways. Environ Pollut. 2018 May;236:273-282. doi: 10.1016/j.envpol.2018.01.056. PubMed PMID: 29414349.

11: Wu S, Lyu T, Zhao Y, Vymazal J, Arias CA, Brix H. Rethinking Intensification of Constructed Wetlands as a Green Eco-Technology for Wastewater Treatment. Environ Sci Technol. 2018 Feb 20;52(4):1693-1694. doi: 10.1021/acs.est.8b00010. Epub 2018 Feb 1. PubMed PMID: 29388763.

12: Bremner-Harrison S, Cypher BL, Van Horn Job C, Harrison SWR. Assessing personality in San Joaquin kit fox in situ: efficacy of field-based experimental methods and implications for conservation management. J Ethol. 2018;36(1):23-33. doi: 10.1007/s10164-017-0525-9. Epub 2017 Sep 12. PubMed PMID: 29353954; PubMed Central PMCID: PMC5746588.

13: Zhang L, Lyu T, Zhang Y, Button M, Arias CA, Weber KP, Brix H, Carvalho PN. Impacts of design configuration and plants on the functionality of the microbial community of mesocosm-scale constructed wetlands treating ibuprofen. Water Res. 2017 Dec 23;131:228-238. doi: 10.1016/j.watres.2017.12.050. [Epub ahead of print] PubMed PMID: 29291484.

14: Wang H, Chen Z, Li Y, Ji Q. NG25, an inhibitor of transforming growth factor β activated kinase 1, ameliorates neuronal apoptosis in neonatal hypoxic ischemic rats. Mol Med Rep. 2018 Jan;17(1):1710-1716. doi: 10.3892/mmr.2017.8024. Epub 2017 Nov 10. PubMed PMID: 29138854; PubMed Central PMCID: PMC5780114.

15: Bi L, Pan G. From harmful Microcystis blooms to multi-functional core-double-shell microsphere bio-hydrochar materials. Sci Rep. 2017 Nov 13;7(1):15477. doi: 10.1038/s41598-017-15696-9. PubMed PMID: 29133868; PubMed Central PMCID: PMC5684341.

16: Ding Y, Lyu T, Bai S, Li Z, Ding H, You S, Xie Q. Effect of multilayer substrate configuration in horizontal subsurface flow constructed wetlands: assessment of treatment performance, biofilm development, and solids accumulation. Environ Sci Pollut Res Int. 2018 Jan;25(2):1883-1891. doi: 10.1007/s11356-017-0636-4. Epub 2017 Nov 4. PubMed PMID: 29103118.

17: Valencia-Avellan M, Slack R, Stockdale A, Mortimer RJG. Evaluating water quality and ecotoxicology assessment techniques using data from a lead and zinc effected upland limestone catchment. Water Res. 2018 Jan 1;128:49-60. doi: 10.1016/j.watres.2017.10.031. Epub 2017 Oct 15. PubMed PMID: 29080409.

18: Hobson AJ, Stewart DI, Bray AW, Mortimer RJG, Mayes WM, Rogerson M, Burke IT. Mechanism of Vanadium Leaching during Surface Weathering of Basic Oxygen Furnace Steel Slag Blocks: A Microfocus X-ray Absorption Spectroscopy and Electron Microscopy Study. Environ Sci Technol. 2017 Jul 18;51(14):7823-7830. doi: 10.1021/acs.est.7b00874. Epub 2017 Jul 6. PubMed PMID: 28627883.

19: Scholey D, Burton E, Morgan N, Sanni C, Madsen CK, Dionisio G, Brinch-Pedersen H. P and Ca digestibility is increased in broiler diets supplemented with the high-phytase HIGHPHY wheat. Animal. 2017 Sep;11(9):1457-1463. doi: 10.1017/S1751731117000544. Epub 2017 Mar 20. PubMed PMID: 28318476.

20: Xu R, Zhang M, Mortimer RJ, Pan G. Enhanced Phosphorus Locking by Novel Lanthanum/Aluminum-Hydroxide Composite: Implications for Eutrophication Control. Environ Sci Technol. 2017 Mar 21;51(6):3418-3425. doi: 10.1021/acs.est.6b05623. Epub 2017 Mar 9. PubMed PMID: 28225266.