E6446 HCl
new
featured

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 555222

CAS#: 1345675-25-3 (HCl)

Description: E6446 is a synthetic antagonist of nucleic acid-sensing TLRs. , In vitro, low doses of E6446 specifically inhibited the activation of human and mouse TLR9. Tenfold higher concentrations of this compound also inhibited the human TLR8 response to single-stranded RNA. In vivo, therapy with E6446 diminished the activation of TLR9 and prevented the exacerbated cytokine response observed during acute Plasmodium infection. Furthermore, severe signs of ECM, such as limb paralysis, brain vascular leak, and death, were all prevented by oral treatment with E6446.

Chemical Structure

img
E6446 HCl
CAS# 1345675-25-3 (HCl)
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 555222
Name: E6446 HCl
CAS#: 1345675-25-3 (HCl)
Chemical Formula: C27H37Cl2N3O3
Exact Mass: 0.00
Molecular Weight: 522.511
Elemental Analysis: C, 62.07; H, 7.14; Cl, 13.57; N, 8.04; O, 9.19

Price and Availability

Size Price Availability Quantity
50mg USD 750 2 weeks
100mg USD 1250 2 Weeks
200mg USD 2250 2 weeks
Bulk inquiry

Related CAS #: 1219925-73-1 (free base)   1345675-25-3 (HCl)    

Synonym: E6446 HCl; E6446; E-6446; E6446 hydrochloride

IUPAC/Chemical Name: 6-(3-pyrrolidin-1-ylpropoxy)-2-[4-(3-pyrrolidin-1-ylpropoxy)phenyl]-1,3-benzoxazole;dihydrochloride

InChi Key: DQOUZINBHKWGGM-UHFFFAOYSA-N

InChi Code: InChI=1S/C27H35N3O3.2ClH/c1-2-14-29(13-1)17-5-19-31-23-9-7-22(8-10-23)27-28-25-12-11-24(21-26(25)33-27)32-20-6-18-30-15-3-4-16-30;;/h7-12,21H,1-6,13-20H2;2*1H

SMILES Code: [H]Cl.[H]Cl.C1(C2=CC=C(OCCCN3CCCC3)C=C2)=NC4=CC=C(OCCCN5CCCC5)C=C4O1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Instruction:
View handling instruction
Biological target: E6446 dihydrochloride is a specific Toll-like receptor 9 inhibitor.
In vitro activity: Measurement of ELAM-1–luciferase activity showed that E6446 specifically inhibited TLR9 activation with CpG ODN 2006, in the range of 0.01–0.03 μM. A 100-fold higher concentration (2–8 μM) of E6446 was required to inhibit TLR7/8 activated by the imidazoquinoline compound R848. Incubation of cells with even higher concentrations (30 μM) of E6446 was required to reduce 50% of TLR4 activation (Fig. 2A). Reference: Proc Natl Acad Sci U S A. 2011 Mar 1; 108(9): 3689–3694. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048158/
In vivo activity: E6446 treatment significantly reduced LV chamber size and improved cardiac function in TAC-operated mice. Reference: JACC Basic Transl Sci. 2019 Jun; 4(3): 348–363. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610159/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.5 14.35
DMF 20.0 38.28
DMF:PBS (pH 7.2) (1:4) 0.2 0.38
Ethanol 2.0 3.83
Water 100.0 191.38

Preparing Stock Solutions

The following data is based on the product molecular weight 522.51 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Ueda H, Yamaguchi O, Taneike M, Akazawa Y, Wada-Kobayashi H, Sugihara R, Yorifuji H, Nakayama H, Omiya S, Murakawa T, Sakata Y, Otsu K. Administration of a TLR9 Inhibitor Attenuates the Development and Progression of Heart Failure in Mice. JACC Basic Transl Sci. 2019 May 22;4(3):348-363. doi: 10.1016/j.jacbts.2019.01.002. PMID: 31312759; PMCID: PMC6610159. 2. Franklin BS, Ishizaka ST, Lamphier M, Gusovsky F, Hansen H, Rose J, Zheng W, Ataíde MA, de Oliveira RB, Golenbock DT, Gazzinelli RT. Therapeutical targeting of nucleic acid-sensing Toll-like receptors prevents experimental cerebral malaria. Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3689-94. doi: 10.1073/pnas.1015406108. Epub 2011 Feb 8. PMID: 21303985; PMCID: PMC3048158. 3. Yoshida K, Abe K, Ishikawa M, Saku K, Shinoda-Sakamoto M, Ishikawa T, Watanabe T, Oka M, Sunagawa K, Tsutsui H. Inhibition of TLR9-NF-κB-mediated sterile inflammation improves pressure overload-induced right ventricular dysfunction in rats. Cardiovasc Res. 2019 Mar 1;115(3):658-668. doi: 10.1093/cvr/cvy209. PMID: 30239623.
In vitro protocol: 1. Ueda H, Yamaguchi O, Taneike M, Akazawa Y, Wada-Kobayashi H, Sugihara R, Yorifuji H, Nakayama H, Omiya S, Murakawa T, Sakata Y, Otsu K. Administration of a TLR9 Inhibitor Attenuates the Development and Progression of Heart Failure in Mice. JACC Basic Transl Sci. 2019 May 22;4(3):348-363. doi: 10.1016/j.jacbts.2019.01.002. PMID: 31312759; PMCID: PMC6610159. 2. Franklin BS, Ishizaka ST, Lamphier M, Gusovsky F, Hansen H, Rose J, Zheng W, Ataíde MA, de Oliveira RB, Golenbock DT, Gazzinelli RT. Therapeutical targeting of nucleic acid-sensing Toll-like receptors prevents experimental cerebral malaria. Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3689-94. doi: 10.1073/pnas.1015406108. Epub 2011 Feb 8. PMID: 21303985; PMCID: PMC3048158.
In vivo protocol: 1. Ueda H, Yamaguchi O, Taneike M, Akazawa Y, Wada-Kobayashi H, Sugihara R, Yorifuji H, Nakayama H, Omiya S, Murakawa T, Sakata Y, Otsu K. Administration of a TLR9 Inhibitor Attenuates the Development and Progression of Heart Failure in Mice. JACC Basic Transl Sci. 2019 May 22;4(3):348-363. doi: 10.1016/j.jacbts.2019.01.002. PMID: 31312759; PMCID: PMC6610159. 2. Yoshida K, Abe K, Ishikawa M, Saku K, Shinoda-Sakamoto M, Ishikawa T, Watanabe T, Oka M, Sunagawa K, Tsutsui H. Inhibition of TLR9-NF-κB-mediated sterile inflammation improves pressure overload-induced right ventricular dysfunction in rats. Cardiovasc Res. 2019 Mar 1;115(3):658-668. doi: 10.1093/cvr/cvy209. PMID: 30239623.

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Franklin BS, Ishizaka ST, Lamphier M, Gusovsky F, Hansen H, Rose J, Zheng W,
Ataíde MA, de Oliveira RB, Golenbock DT, Gazzinelli RT. Therapeutical targeting
of nucleic acid-sensing Toll-like receptors prevents experimental cerebral
malaria. Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3689-94. doi:
10.1073/pnas.1015406108. Epub 2011 Feb 8. PubMed PMID: 21303985; PubMed Central
PMCID: PMC3048158.