RI-1 | CAS#415713-60-9 | RAD51 inhibitor

RI-1
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 407511

CAS#: 415713-60-9

Description: RI-1 is an irreversible inhibitor of RAD51. RI-1 holds promise as an oncologic drug. RI-1 represents a unique tool to dissect the network of reaction pathways that contribute to DNA repair in cells.

Chemical Structure

RI-1

CAS# 415713-60-9

Product data Instruction

Theoretical Analysis

MedKoo Cat#: 407511
Name: RI-1
CAS#: 415713-60-9
Chemical Formula: C14H11Cl3N2O3
Exact Mass: 359.98
Molecular Weight: 361.600
Elemental Analysis: C, 46.50; H, 3.07; Cl, 29.41; N, 7.75; O, 13.27

Price and Availability

Size	Price	Availability
50mg	USD 250	2 weeks
100mg	USD 450	2 weeks
200mg	USD 750	2 weeks
500mg	USD 1450	2 weeks
1g	USD 2450	2 weeks
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: RI-1; RI1; RI 1.

IUPAC/Chemical Name: 3-chloro-1-(3,4-dichlorophenyl)-4-(4-morpholinyl)-1H-pyrrole-2,5-dione

InChi Key: MWSUIZKGNWELRF-UHFFFAOYSA-N

InChi Code: InChI=1S/C14H11Cl3N2O3/c15-9-2-1-8(7-10(9)16)19-13(20)11(17)12(14(19)21)18-3-5-22-6-4-18/h1-2,7H,3-6H2

SMILES Code: O=C(C(Cl)=C1N2CCOCC2)N(C3=CC=C(Cl)C(Cl)=C3)C1=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:

Product Data

Instruction:

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Biological target:	RI-1 is an irreversible inhibitor of RAD51 (IC50s = 5-30 µM). RI-1 specifically reduces gene conversion in human cells while stimulating single strand annealing. RI-1 binds covalently to the surface of RAD51 protein at cysteine 319 that likely destabilizes an interface used by RAD51 monomers to oligomerize into filaments on DNA. RI-1 holds promise as an oncologic drug.
In vitro activity:	RI-1 potentiates the lethal effects of a DNA cross-linking drug in human cells. RI-1 represents a unique tool to dissect the network of reaction pathways that contribute to DNA repair in cells. Reference: Nucleic Acids Res. 2012 Aug;40(15):7347-57. https://pubmed.ncbi.nlm.nih.gov/22573178/
In vivo activity:	To be determined

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMF	13.0	35.95
	DMSO	2.0	5.53

Preparing Stock Solutions

The following data is based on the product molecular weight 361.60 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:	1. Budke B, Logan HL, Kalin JH, Zelivianskaia AS, Cameron McGuire W, Miller LL, Stark JM, Kozikowski AP, Bishop DK, Connell PP. RI-1: a chemical inhibitor of RAD51 that disrupts homologous recombination in human cells. Nucleic Acids Res. 2012 Aug;40(15):7347-57. doi: 10.1093/nar/gks353. Epub 2012 May 9. PMID: 22573178; PMCID: PMC3424541.
In vitro protocol:	1. Budke B, Logan HL, Kalin JH, Zelivianskaia AS, Cameron McGuire W, Miller LL, Stark JM, Kozikowski AP, Bishop DK, Connell PP. RI-1: a chemical inhibitor of RAD51 that disrupts homologous recombination in human cells. Nucleic Acids Res. 2012 Aug;40(15):7347-57. doi: 10.1093/nar/gks353. Epub 2012 May 9. PMID: 22573178; PMCID: PMC3424541.
In vivo protocol:	To be determined

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1: Budke B, Logan HL, Kalin JH, Zelivianskaia AS, Cameron McGuire W, Miller LL,
Stark JM, Kozikowski AP, Bishop DK, Connell PP. RI-1: a chemical inhibitor of
RAD51 that disrupts homologous recombination in human cells. Nucleic Acids Res.
2012 Aug;40(15):7347-57. doi: 10.1093/nar/gks353. Epub 2012 May 9. PubMed PMID:
22573178; PubMed Central PMCID: PMC3424541.

2: Golec P, Wiczk A, Majchrzyk A, Łoś JM, Węgrzyn G, Łoś M. A role for accessory
genes rI.-1 and rI.1 in the regulation of lysis inhibition by bacteriophage T4.
Virus Genes. 2010 Dec;41(3):459-68. doi: 10.1007/s11262-010-0532-1. Epub 2010 Oct
14. PubMed PMID: 20945083; PubMed Central PMCID: PMC2962797.

3: Bartmann P, Bamberger U, Pohlandt F, Gortner L. Immunogenicity and
immunomodulatory activity of bovine surfactant (SF-RI 1). Acta Paediatr. 1992
May;81(5):383-8. PubMed PMID: 1498502.

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