Wybutine

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 341263

CAS#: 33359-03-4

Description: Wybutine, also known as YWye or Wye, is a Fluorescent base isolated from Torulopsis utilis. YWye by the size of its aromatic system and by its stacking properties is particularly well suited for stabilizing the native conformation of tRNAPhe.


Chemical Structure

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Wybutine
CAS# 33359-03-4

Theoretical Analysis

MedKoo Cat#: 341263
Name: Wybutine
CAS#: 33359-03-4
Chemical Formula: C9H9N5O
Exact Mass: 203.0807
Molecular Weight: 203.205
Elemental Analysis: C, 53.20; H, 4.46; N, 34.47; O, 7.87

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: Wye; Wybutine; Y Base; YWye,

IUPAC/Chemical Name: 9H-Imidazo(1,2-a)purin-9-one, 1,4-dihydro-4,6-dimethyl-

InChi Key: ZLHLYESIHSHXGM-UHFFFAOYSA-N

InChi Code: InChI=1S/C9H9N5O/c1-5-3-14-8(15)6-7(11-4-10-6)13(2)9(14)12-5/h3-4H,1-2H3,(H,10,11)

SMILES Code: O=C1N2C(N(C)C3=C1NC=N3)=NC(C)=C2

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 203.205 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1: Kumbhar NM, Sonawane KD. Iso-energetic multiple conformations of hypermodified
nucleic acid base wybutine (yW) which occur at 37(th) position in anticodon loop
of tRNA(Phe). J Mol Graph Model. 2011 Jun;29(7):935-46. doi:
10.1016/j.jmgm.2011.03.005. Epub 2011 Apr 12. PubMed PMID: 21530341.

2: Claesens F, Rigler R. Conformational dynamics of the anticodon loop in yeast
tRNAPhe as sensed by the fluorescence of wybutine. Eur Biophys J.
1986;13(6):331-42. PubMed PMID: 3530734.

3: Paulsen H, Robertson JM, Wintermeyer W. Effect of ribosome binding and
translocation on the anticodon of tRNAPhe as studied by wybutine fluorescence.
Nucleic Acids Res. 1982 Apr 24;10(8):2651-63. PubMed PMID: 7043399; PubMed
Central PMCID: PMC320640.

4: Ehrlich R, Lefevre JF, Remy P. Fluorimetric study of the complex between yeast
phenylalanyl-tRNA synthetase and tRNA-Phe. 1. Changes in the conformation of the
enzyme and tRNA; modification of the Wybutine neighbourhood. Eur J Biochem. 1980
Jan;103(1):145-53. PubMed PMID: 6987055.

5: Wintermeyer W, Schleich HG, Zachau HG. Incorporation of amines or hydrazines
into tRNA replacing wybutine or dihydrouracil. Methods Enzymol. 1979;59:110-21.
PubMed PMID: 440077.

6: Schleich HG, Wintermeyer W, Zachau HG. Replacement of wybutine by hydrazines
and its effect on the active conformation of yeast tRNAPhe. Nucleic Acids Res.
1978 May;5(5):1701-13. PubMed PMID: 351568; PubMed Central PMCID: PMC342114.