NP-313
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MedKoo CAT#: 562405

CAS#: 5397-78-4

Description: NP-313 is an antithrombotic agent with dual inhibition of thromboxane A2 synthesis and calcium entry.

Chemical Structure

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NP-313
CAS# 5397-78-4
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 562405
Name: NP-313
CAS#: 5397-78-4
Chemical Formula: C12H8ClNO3
Exact Mass: 249.02
Molecular Weight: 249.650
Elemental Analysis: C, 57.73; H, 3.23; Cl, 14.20; N, 5.61; O, 19.23

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5mg USD 260
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Synonym: NP-313; NP 313; NP313; NSC-4264; NSC 4264; NSC4264;

IUPAC/Chemical Name: 2-Acetylamino-3-chloro-1,4-naphthoquinone

InChi Key: OMXMYDUYAUOFRX-UHFFFAOYSA-N

InChi Code: InChI=1S/C12H8ClNO3/c1-6(15)14-10-9(13)11(16)7-4-2-3-5-8(7)12(10)17/h2-5H,1H3,(H,14,15)

SMILES Code: O=C(C1=C2C=CC=C1)C(Cl)=C(NC(C)=O)C2=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Instruction:
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Biological target: NP-313 is a potent antithrombotic agent that inhibits platelet aggregation and activation.
In vitro activity: NP-313 concentration-dependently inhibited human platelet aggregation induced by collagen, arachidonic acid, thapsigargin, thrombin and A23187. NP-313 also inhibited P-selectin expression, thromboxane B(2) formation and [Ca(2+) ](i) elevation in platelets stimulated by thrombin and collagen. NP-313 at 10 µM inhibited cyclooxygenase, thromboxane A(2) synthase, and protein kinase Cα, whereas it did not affect phospholipase A(2) or phospholipase C activity. Reference: Br J Pharmacol. 2011 Apr;162(8):1871-83. https://pubmed.ncbi.nlm.nih.gov/21232029/
In vivo activity: This study evaluated the ex vivo antiplatelet action of NP-313 on PRP. As shown in Figure 9C, upon i.v. administration of NP-313 at 4, 8 and 16 µg·g−1 for mice, NP-313 inhibited ex vivo platelet aggregation of PRP caused by collagen (10 µg·mL−1) 5 min after drug administration. Reference: Br J Pharmacol. 2011 Apr;162(8):1871-83. https://pubmed.ncbi.nlm.nih.gov/21232029/

Preparing Stock Solutions

The following data is based on the product molecular weight 249.65 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: Kuo HL, Lien JC, Chang CH, Chung CH, Kuo SC, Hsu CC, Peng HC, Huang TF. NP-313, 2-acetylamino-3-chloro-1,4-naphthoquinone, a novel antithrombotic agent with dual inhibition of thromboxane A(2) synthesis and calcium entry. Br J Pharmacol. 2011 Apr;162(8):1871-83. doi: 10.1111/j.1476-5381.2011.01200.x. PMID: 21232029; PMCID: PMC3081128.
In vitro protocol: Kuo HL, Lien JC, Chang CH, Chung CH, Kuo SC, Hsu CC, Peng HC, Huang TF. NP-313, 2-acetylamino-3-chloro-1,4-naphthoquinone, a novel antithrombotic agent with dual inhibition of thromboxane A(2) synthesis and calcium entry. Br J Pharmacol. 2011 Apr;162(8):1871-83. doi: 10.1111/j.1476-5381.2011.01200.x. PMID: 21232029; PMCID: PMC3081128.
In vivo protocol: Kuo HL, Lien JC, Chang CH, Chung CH, Kuo SC, Hsu CC, Peng HC, Huang TF. NP-313, 2-acetylamino-3-chloro-1,4-naphthoquinone, a novel antithrombotic agent with dual inhibition of thromboxane A(2) synthesis and calcium entry. Br J Pharmacol. 2011 Apr;162(8):1871-83. doi: 10.1111/j.1476-5381.2011.01200.x. PMID: 21232029; PMCID: PMC3081128.

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1: Werner WJ, Allen KD, Hu K, Helms GL, Chen BS, Wang SC. In vitro phosphinate methylation by PhpK from Kitasatospora phosalacinea. Biochemistry. 2011 Oct 25;50(42):8986-8. doi: 10.1021/bi201220r. Epub 2011 Sep 28. PubMed PMID: 21950770; PubMed Central PMCID: PMC3214642.

2: Kuo HL, Lien JC, Chang CH, Chung CH, Kuo SC, Hsu CC, Peng HC, Huang TF. NP-313, 2-acetylamino-3-chloro-1,4-naphthoquinone, a novel antithrombotic agent with dual inhibition of thromboxane A(2) synthesis and calcium entry. Br J Pharmacol. 2011 Apr;162(8):1871-83. doi: 10.1111/j.1476-5381.2011.01200.x. PubMed PMID: 21232029; PubMed Central PMCID: PMC3081128.