SAL003
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MedKoo CAT#: 530967

CAS#: 1164470-53-4

Description: SAL003 is a cell-permeable inhibitor of eIF2α dephosphorylation. SAL003 is an Analog of salubrinal with improved aqueous solubility. SAL003‑mediated upregulation of eIF2α phosphorylation suppressed cisplatin‑induced p53 activation. Furthermore, reduction of eIF2α phosphorylation by PERK knockdown enhanced cisplatin-induced p53 activation and apoptosis.


Chemical Structure

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SAL003
CAS# 1164470-53-4

Theoretical Analysis

MedKoo Cat#: 530967
Name: SAL003
CAS#: 1164470-53-4
Chemical Formula: C18H15Cl4N3OS
Exact Mass: 460.97
Molecular Weight: 463.200
Elemental Analysis: C, 46.68; H, 3.26; Cl, 30.61; N, 9.07; O, 3.45; S, 6.92

Price and Availability

Size Price Availability Quantity
1mg USD 400 2 Weeks
10mg USD 600 2 Weeks
50mg USD 1250 2 Weeks
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Synonym: SAL-003; SAL 003; SAL003.

IUPAC/Chemical Name: 3--Phenyl-N-(2,2,2-trichloro-1-((((4-chlorophenyl)amino)carbonothioyl)amino)ethyl)acrylamide

InChi Key: TVNBASWNLOIQML-IZZDOVSWSA-N

InChi Code: InChI=1S/C18H15Cl4N3OS/c19-13-7-9-14(10-8-13)23-17(27)25-16(18(20,21)22)24-15(26)11-6-12-4-2-1-3-5-12/h1-11,16H,(H,24,26)(H2,23,25,27)/b11-6+

SMILES Code: O=C(NC(NC(NC1=CC=C(Cl)C=C1)=S)C(Cl)(Cl)Cl)/C=C/C2=CC=CC=C2

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info: Cisplatin is one of the most widely used chemotherapeutic agents for the treatment of human cancers. However, the nephrotoxicity of cisplatin limits its use as a therapeutic agent. It has been suggested that oxidative stress and p53 activation play important roles in cisplatin-induced nephrotoxicity. It has been demonstrated that the eukaryotic translation initiation factor 2α (eIF2α) may protect HK-2 human renal proximal tubular cells against cisplatin-induced apoptosis through inhibition of reactive oxygen species (ROS)‑mediated p53 activation.

Product Data:
Biological target: Sakuranetin is a cherry flavonoid phytoalexin that shows strong antifungal activity. Sakuranetin has anti-inflammatory and antioxidative activities. Sakuranetin ameliorates LPS-induced acute lung injury.
In vitro activity: In HeLa cells, knockdown of eIF2α by siRNA or inhibition of eIF2α dephosphorylation by SAL003 enhanced SubAB-induced caspase activation. Reference: Infect Immun. 2012 May;80(5):1803-14. https://pubmed.ncbi.nlm.nih.gov/22354021/
In vivo activity: Only early-long-term potentiationis evoked by repeated tetanic stimulation and long term memory is impaired when eIF2α phosphorylation is increased by injecting SAL003 into the hippocampus, which prevents the dephosphorylation of eIF2α. Reference: Cell. 2007 Apr 6;129(1):195-206. https://pubmed.ncbi.nlm.nih.gov/17418795/

Preparing Stock Solutions

The following data is based on the product molecular weight 463.20 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Soiberman US, Shehata AEM, Lu MX, Young T, Daoud YJ, Chakravarti S, Jun AS, Foster JW. Small Molecule Modulation of the Integrated Stress Response Governs the Keratoconic Phenotype In Vitro. Invest Ophthalmol Vis Sci. 2019 Aug 1;60(10):3422-3431. doi: 10.1167/iovs.19-27151. PMID: 31390655; PMCID: PMC6686743. 2. Yahiro K, Tsutsuki H, Ogura K, Nagasawa S, Moss J, Noda M. Regulation of subtilase cytotoxin-induced cell death by an RNA-dependent protein kinase-like endoplasmic reticulum kinase-dependent proteasome pathway in HeLa cells. Infect Immun. 2012 May;80(5):1803-14. doi: 10.1128/IAI.06164-11. Epub 2012 Feb 21. PMID: 22354021; PMCID: PMC3347452. 3. Peterson C, Kim YC, Ensign LM, Jun AS, Foster J. Induction of the integrated stress response in the rat cornea. Exp Eye Res. 2021 Sep;210:108722. doi: 10.1016/j.exer.2021.108722. Epub 2021 Aug 8. PMID: 34370978; PMCID: PMC8429264. 4. Costa-Mattioli M, Gobert D, Stern E, Gamache K, Colina R, Cuello C, Sossin W, Kaufman R, Pelletier J, Rosenblum K, Krnjević K, Lacaille JC, Nader K, Sonenberg N. eIF2alpha phosphorylation bidirectionally regulates the switch from short- to long-term synaptic plasticity and memory. Cell. 2007 Apr 6;129(1):195-206. doi: 10.1016/j.cell.2007.01.050. PMID: 17418795; PMCID: PMC4149214.
In vitro protocol: 1. Soiberman US, Shehata AEM, Lu MX, Young T, Daoud YJ, Chakravarti S, Jun AS, Foster JW. Small Molecule Modulation of the Integrated Stress Response Governs the Keratoconic Phenotype In Vitro. Invest Ophthalmol Vis Sci. 2019 Aug 1;60(10):3422-3431. doi: 10.1167/iovs.19-27151. PMID: 31390655; PMCID: PMC6686743. 2. Yahiro K, Tsutsuki H, Ogura K, Nagasawa S, Moss J, Noda M. Regulation of subtilase cytotoxin-induced cell death by an RNA-dependent protein kinase-like endoplasmic reticulum kinase-dependent proteasome pathway in HeLa cells. Infect Immun. 2012 May;80(5):1803-14. doi: 10.1128/IAI.06164-11. Epub 2012 Feb 21. PMID: 22354021; PMCID: PMC3347452.
In vivo protocol: 1. Peterson C, Kim YC, Ensign LM, Jun AS, Foster J. Induction of the integrated stress response in the rat cornea. Exp Eye Res. 2021 Sep;210:108722. doi: 10.1016/j.exer.2021.108722. Epub 2021 Aug 8. PMID: 34370978; PMCID: PMC8429264. 2. Costa-Mattioli M, Gobert D, Stern E, Gamache K, Colina R, Cuello C, Sossin W, Kaufman R, Pelletier J, Rosenblum K, Krnjević K, Lacaille JC, Nader K, Sonenberg N. eIF2alpha phosphorylation bidirectionally regulates the switch from short- to long-term synaptic plasticity and memory. Cell. 2007 Apr 6;129(1):195-206. doi: 10.1016/j.cell.2007.01.050. PMID: 17418795; PMCID: PMC4149214.

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1: Ju SM, Jo YS, Jeon YM, Pae HO, Kang DG, Lee HS, Bae JS, Jeon BH.
Phosphorylation of eIF2α suppresses cisplatin-induced p53 activation and
apoptosis by attenuating oxidative stress via ATF4-mediated HO-1 expression in
human renal proximal tubular cells. Int J Mol Med. 2017 Oct 10. doi:
10.3892/ijmm.2017.3181. [Epub ahead of print] PubMed PMID: 29039478.


2: Jian M, Luo YX, Xue YX, Han Y, Shi HS, Liu JF, Yan W, Wu P, Meng SQ, Deng JH,
Shen HW, Shi J, Lu L. eIF2α dephosphorylation in basolateral amygdala mediates
reconsolidation of drug memory. J Neurosci. 2014 Jul 23;34(30):10010-21. doi:
10.1523/JNEUROSCI.0934-14.2014. PubMed PMID: 25057203.


3: Costa-Mattioli M, Gobert D, Stern E, Gamache K, Colina R, Cuello C, Sossin W,
Kaufman R, Pelletier J, Rosenblum K, Krnjević K, Lacaille JC, Nader K, Sonenberg
N. eIF2alpha phosphorylation bidirectionally regulates the switch from short- to
long-term synaptic plasticity and memory. Cell. 2007 Apr 6;129(1):195-206. PubMed
PMID: 17418795; PubMed Central PMCID: PMC4149214.


4: Yahiro K, Tsutsuki H, Ogura K, Nagasawa S, Moss J, Noda M. Regulation of
subtilase cytotoxin-induced cell death by an RNA-dependent protein kinase-like
endoplasmic reticulum kinase-dependent proteasome pathway in HeLa cells. Infect
Immun. 2012 May;80(5):1803-14. doi: 10.1128/IAI.06164-11. Epub 2012 Feb 21.
PubMed PMID: 22354021; PubMed Central PMCID: PMC3347452.