MedKoo Biosciences

Puromycin Dihydrochloride
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 561474

CAS#: 58-58-2

Description: Puromycin Dihydrochloride is an antibiotic. It inhibits protein synthesis by binding to RNA. Puromycin Dihydrochloride has antimicrobial, antitrypanosomal, and antineoplastic properties.

Chemical Structure

Puromycin Dihydrochloride

CAS# 58-58-2

Product data Instruction

Theoretical Analysis

MedKoo Cat#: 561474
Name: Puromycin Dihydrochloride
CAS#: 58-58-2
Chemical Formula: C22H31Cl2N7O5
Exact Mass: 0.00
Molecular Weight: 544.430
Elemental Analysis: C, 48.54; H, 5.74; Cl, 13.02; N, 18.01; O, 14.69

Price and Availability

Size	Price	Availability	Quantity
50mg	USD 200
100mg	USD 330
500mg	USD 710
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: Puromycin Dihydrochloride; Puromycin 2HCl;

IUPAC/Chemical Name: (2S)-2-Amino-N-[(2S,3S,4R,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide dihydrochloride

InChi Key: MKSVFGKWZLUTTO-FZFAUISWSA-N

InChi Code: InChI=1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1

SMILES Code: O=C(N[C@@H]1[C@@H](CO)O[C@@H](N2C=NC3=C(N(C)C)N=CN=C23)[C@@H]1O)[C@@H](N)CC4=CC=C(OC)C=C4.[H]Cl.[H]Cl

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, ethanol, methanol, and water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:

Product Data

Instruction:

View handling instruction

Biological target:	Puromycin inhibits protein synthesis by binding to RNA. It is an antineoplastic and antitrypanosomal agent and is used in research as an inhibitor of protein synthesis. It also serves an an anticancer drug by suppressing the growth and metastasis in esophagus cancer cells by Akt phosphorylation.
In vitro activity:	The effects of Puromycin aminonucleoside (PAN) were investigated on podocytes in the context of diabetic nephropathy. PAN treatment had a dose- and time-dependent inhibitory effect on podocyte proliferation, suggesting its role in suppressing cell growth. KLF5 overexpression induced cell cycle arrest in podocytes when regulated by PAN. KLF5 overexpression was shown to inhibit PAN-induced apoptosis of podocyte. Reference: Mol Med Rep. 2018 Oct;18(4):3843-3849. https://pubmed.ncbi.nlm.nih.gov/30106142/
In vivo activity:	In adult zebrafish, puromycin administration led to kidney injury, causing rapid clearance of substances from the kidney and resulting in morphological changes in the kidney structure, which recovered over time. During the process of nephron injury and regeneration, various molecular markers associated with kidney injury and repair showed significant changes, including increased expression of renal progenitor marker lhx1a and upregulation of NFκB, TNFα, Nampt, and p-ERK. Reference: Anim Cells Syst (Seoul). 2023 Apr 20;27(1):112-119. https://pubmed.ncbi.nlm.nih.gov/37089626/

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	50.0	91.84
	Ethanol	5.0	9.18
	Methanol	250.0	459.20
	Water	50.0	91.84

Preparing Stock Solutions

The following data is based on the product molecular weight 544.43 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:	1. Li Y, Sui X, Hu X, Hu Z. Overexpression of KLF5 inhibits puromycin‑induced apoptosis of podocytes. Mol Med Rep. 2018 Oct;18(4):3843-3849. doi: 10.3892/mmr.2018.9366. Epub 2018 Aug 9. PMID: 30106142; PMCID: PMC6131625. 2. Hernández-Damián J, Tecalco-Cruz AC, Ríos-López DG, Vázquez-Victorio G, Vázquez-Macías A, Caligaris C, Sosa-Garrocho M, Flores-Pérez B, Romero-Avila M, Macías-Silva M. Downregulation of SnoN oncoprotein induced by antibiotics anisomycin and puromycin positively regulates transforming growth factor-β signals. Biochim Biophys Acta. 2013 Nov;1830(11):5049-58. doi: 10.1016/j.bbagen.2013.07.006. Epub 2013 Jul 18. PMID: 23872350. 3. Koun S, Park HJ, Jung SM, Cha JJ, Cha DR, Kang YS. Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish. Anim Cells Syst (Seoul). 2023 Apr 20;27(1):112-119. doi: 10.1080/19768354.2023.2203211. PMID: 37089626; PMCID: PMC10120544. 4. Sakamoto H, Hirakawa Y, Ishida KI, Keeling PJ, Kita K, Matsuzaki M. Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol Int. 2019 Apr;69:13-16. doi: 10.1016/j.parint.2018.10.011. Epub 2018 Oct 30. PMID: 30389616.
In vitro protocol:	1. Li Y, Sui X, Hu X, Hu Z. Overexpression of KLF5 inhibits puromycin‑induced apoptosis of podocytes. Mol Med Rep. 2018 Oct;18(4):3843-3849. doi: 10.3892/mmr.2018.9366. Epub 2018 Aug 9. PMID: 30106142; PMCID: PMC6131625. 2. Hernández-Damián J, Tecalco-Cruz AC, Ríos-López DG, Vázquez-Victorio G, Vázquez-Macías A, Caligaris C, Sosa-Garrocho M, Flores-Pérez B, Romero-Avila M, Macías-Silva M. Downregulation of SnoN oncoprotein induced by antibiotics anisomycin and puromycin positively regulates transforming growth factor-β signals. Biochim Biophys Acta. 2013 Nov;1830(11):5049-58. doi: 10.1016/j.bbagen.2013.07.006. Epub 2013 Jul 18. PMID: 23872350.
In vivo protocol:	1. Koun S, Park HJ, Jung SM, Cha JJ, Cha DR, Kang YS. Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish. Anim Cells Syst (Seoul). 2023 Apr 20;27(1):112-119. doi: 10.1080/19768354.2023.2203211. PMID: 37089626; PMCID: PMC10120544. 2. Sakamoto H, Hirakawa Y, Ishida KI, Keeling PJ, Kita K, Matsuzaki M. Puromycin selection for stable transfectants of the oyster-infecting parasite Perkinsus marinus. Parasitol Int. 2019 Apr;69:13-16. doi: 10.1016/j.parint.2018.10.011. Epub 2018 Oct 30. PMID: 30389616.

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1: Stepanenko AA, Heng HH. Transient and stable vector transfection: Pitfalls, off-target effects, artifacts. Mutat Res. 2017 Jul;773:91-103. doi: 10.1016/j.mrrev.2017.05.002. Epub 2017 May 16. Review. PubMed PMID: 28927539.

2: Burford JL, Gyarmati G, Shirato I, Kriz W, Lemley KV, Peti-Peterdi J. Combined use of electron microscopy and intravital imaging captures morphological and functional features of podocyte detachment. Pflugers Arch. 2017 Aug;469(7-8):965-974. doi: 10.1007/s00424-017-2020-0. Epub 2017 Jun 29. Review. PubMed PMID: 28664407; PubMed Central PMCID: PMC5553195.

3: Dinos GP, Athanassopoulos CM, Missiri DA, Giannopoulou PC, Vlachogiannis IA, Papadopoulos GE, Papaioannou D, Kalpaxis DL. Chloramphenicol Derivatives as Antibacterial and Anticancer Agents: Historic Problems and Current Solutions. Antibiotics (Basel). 2016 Jun 3;5(2). pii: E20. doi: 10.3390/antibiotics5020020. Review. PubMed PMID: 27271676; PubMed Central PMCID: PMC4929435.

4: Drainas D. Antibiotics and RNase P. Antibiotics (Basel). 2016 May 6;5(2). pii: E15. doi: 10.3390/antibiotics5020015. Review. PubMed PMID: 27164152; PubMed Central PMCID: PMC4929430.

5: Bertelli R, Bonanni A, Di Donato A, Cioni M, Ravani P, Ghiggeri GM. Regulatory T cells and minimal change nephropathy: in the midst of a complex network. Clin Exp Immunol. 2016 Feb;183(2):166-74. doi: 10.1111/cei.12675. Epub 2015 Oct 12. Review. PubMed PMID: 26147676; PubMed Central PMCID: PMC4711159.

6: Fukusumi Y, Miyauchi N, Hashimoto T, Saito A, Kawachi H. Therapeutic target for nephrotic syndrome: Identification of novel slit diaphragm associated molecules. World J Nephrol. 2014 Aug 6;3(3):77-84. doi: 10.5527/wjn.v3.i3.77. Review. PubMed PMID: 25332898; PubMed Central PMCID: PMC4202494.

7: Agrawal N, Brown MA. Genetic associations and functional characterization of M1 aminopeptidases and immune-mediated diseases. Genes Immun. 2014 Dec;15(8):521-7. doi: 10.1038/gene.2014.46. Epub 2014 Aug 21. Review. PubMed PMID: 25142031.

8: Dever TE, Gutierrez E, Shin BS. The hypusine-containing translation factor eIF5A. Crit Rev Biochem Mol Biol. 2014 Sep-Oct;49(5):413-25. doi: 10.3109/10409238.2014.939608. Epub 2014 Jul 17. Review. PubMed PMID: 25029904; PubMed Central PMCID: PMC4183722.

9: Hitzerd SM, Verbrugge SE, Ossenkoppele G, Jansen G, Peters GJ. Positioning of aminopeptidase inhibitors in next generation cancer therapy. Amino Acids. 2014 Apr;46(4):793-808. doi: 10.1007/s00726-013-1648-0. Epub 2014 Jan 3. Review. PubMed PMID: 24385243.

10: de Mik SM, Hoogduijn MJ, de Bruin RW, Dor FJ. Pathophysiology and treatment of focal segmental glomerulosclerosis: the role of animal models. BMC Nephrol. 2013 Apr 1;14:74. doi: 10.1186/1471-2369-14-74. Review. PubMed PMID: 23547922; PubMed Central PMCID: PMC3637050.

11: Chopra A. (44)Sc-Labeled 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid–conjugated puromycin. 2013 Jan 28 [updated 2013 Feb 28]. Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004-2013. Available from http://www.ncbi.nlm.nih.gov/books/NBK126078/ PubMed PMID: 23469380.

12: Nagarajan NA, Shastri N. Immune surveillance for ERAAP dysfunction. Mol Immunol. 2013 Sep;55(2):120-2. doi: 10.1016/j.molimm.2012.10.006. Epub 2013 Feb 20. Review. PubMed PMID: 23433779; PubMed Central PMCID: PMC3612377.

13: Goodman CA, Hornberger TA. Measuring protein synthesis with SUnSET: a valid alternative to traditional techniques? Exerc Sport Sci Rev. 2013 Apr;41(2):107-15. doi: 10.1097/JES.0b013e3182798a95. Review. PubMed PMID: 23089927; PubMed Central PMCID: PMC3951011.

14: Chao JA, Yoon YJ, Singer RH. Imaging translation in single cells using fluorescent microscopy. Cold Spring Harb Perspect Biol. 2012 Nov 1;4(11). pii: a012310. doi: 10.1101/cshperspect.a012310. Review. PubMed PMID: 22960595; PubMed Central PMCID: PMC3536344.

15: Svenningsen P, Skøtt O, Jensen BL. Proteinuric diseases with sodium retention: Is plasmin the link? Clin Exp Pharmacol Physiol. 2012 Jan;39(1):117-24. doi: 10.1111/j.1440-1681.2011.05524.x. Review. PubMed PMID: 21466573.

16: Peer WA. The role of multifunctional M1 metallopeptidases in cell cycle progression. Ann Bot. 2011 May;107(7):1171-81. doi: 10.1093/aob/mcq265. Epub 2011 Jan 21. Review. PubMed PMID: 21258033; PubMed Central PMCID: PMC3091800.

17: Van Hateren A, James E, Bailey A, Phillips A, Dalchau N, Elliott T. The cell biology of major histocompatibility complex class I assembly: towards a molecular understanding. Tissue Antigens. 2010 Oct;76(4):259-75. doi: 10.1111/j.1399-0039.2010.01550.x. Review. Erratum in: Tissue Antigens. 2010 Nov;76(5):428. PubMed PMID: 21050182.

18: Kodama Y, Fujishima M. Secondary symbiosis between Paramecium and Chlorella cells. Int Rev Cell Mol Biol. 2010;279:33-77. doi: 10.1016/S1937-6448(10)79002-X. Epub 2010 Jan 29. Review. PubMed PMID: 20797676.

19: Yan S, Zhang Y, Lu H, Dong X, Tang C, Mu J. [In vitro display technologies]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2009 Dec;26(6):1367-71. Review. Chinese. PubMed PMID: 20095505.

20: Cybulsky AV. Endoplasmic reticulum stress in proteinuric kidney disease. Kidney Int. 2010 Feb;77(3):187-93. doi: 10.1038/ki.2009.389. Epub 2009 Oct 7. Review. PubMed PMID: 19812538.

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Puromycin Dihydrochloride featured