BML-284 HCl | CAS 2095432-75-8 | Wnt signaling activator

BML-284 HCl
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 561455

CAS#: 2095432-75-8 (HCl)

Description: BML-284, also known as AMBMP and Wnt agonist 1, is a potent and selective activator of Wnt signaling. It enhances beta-catenin and increases transcript and protein levels of p-gp.

Chemical Structure

BML-284 HCl

CAS# 2095432-75-8 (HCl)

Product data Instruction

Theoretical Analysis

MedKoo Cat#: 561455
Name: BML-284 HCl
CAS#: 2095432-75-8 (HCl)
Chemical Formula: C19H19ClN4O3
Exact Mass: 0.00
Molecular Weight: 386.840
Elemental Analysis: C, 58.99; H, 4.95; Cl, 9.16; N, 14.48; O, 12.41

Price and Availability

Size	Price	Availability
25mg	USD 150	2 weeks
50mg	USD 250	2 weeks
100mg	USD 450	2 weeks
200mg	USD 850	2 weeks
500mg	USD 1550	2 weeks
1g	USD 2850	2 weeks
2g	USD 4250	2 weeks
5g	USD 6950	2 weeks
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Related CAS #: 853220-52-7 (free base) 2095432-75-8 (HCl)

Synonym: AMBMP HCl; AMBMP Hydrochloride; BML284 HCl; Wnt Agonist; BML284 HCl; BML-284 HCl; BML 284 HCl; Wnt agonist 1;

IUPAC/Chemical Name: 4-N-(1,3-Benzodioxol-5-ylmethyl)-6-(3-methoxyphenyl)pyrimidine-2,4-diamine Hydrochloride

InChi Key: XZOFNDFDGVAIEH-UHFFFAOYSA-N

InChi Code: InChI=1S/C19H18N4O3.ClH/c1-24-14-4-2-3-13(8-14)15-9-18(23-19(20)22-15)21-10-12-5-6-16-17(7-12)26-11-25-16;/h2-9H,10-11H2,1H3,(H3,20,21,22,23);1H

SMILES Code: NC1=NC(C2=CC=CC(OC)=C2)=CC(NCC3=CC=C(OCO4)C4=C3)=N1.[H]Cl

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:

Product Data

Instruction:

View handling instruction

Biological target:	BML-284 is a Wnt signaling activator and induces TCF-dependent transcriptional activity with an EC50 of 700 nM.
In vitro activity:	Wnt/β-catenin pathway activator BML-284 effectively reversed the effects of hUMSC-Ex-miR-100-5p on cell progression and inflammation in eosinophils. Reference: Acta Biochim Biophys Sin (Shanghai). 2021 Jul 13:gmab093. https://pubmed.ncbi.nlm.nih.gov/34254638/
In vivo activity:	The results demonstrated that insulin promoted corneal epithelial wound healing and sensation recovery, whereas the expression of molecules involved in the Wnt/β-catenin pathway was also up-regulated in the injured corneal epithelium. However, XAV-939 limited the insulin-induced epithelial and corneal nerve repair. By contrast, BML-284 treatment promoted the healing of the corneal epithelium and corneal nerve repair in diabetic mice. Reference: Am J Pathol. 2020 Nov;190(11):2237-2250. https://pubmed.ncbi.nlm.nih.gov/32858016/

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	55.0	142.18
	DMF	30.0	77.55
	Ethanol	0.1	0.36

Preparing Stock Solutions

The following data is based on the product molecular weight 386.84 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:	1. Gao H, Yu Z, Li Y, Wang X. miR-100-5p in human umbilical cord mesenchymal stem cell-derived exosomes mediates eosinophilic inflammation to alleviate atherosclerosis via the FZD5/Wnt/β-catenin pathway. Acta Biochim Biophys Sin (Shanghai). 2021 Jul 13:gmab093. doi: 10.1093/abbs/gmab093. Epub ahead of print. PMID: 34254638. 2. Liu C, Chen L, Wang W, Qin D, Jia C, Yuan M, Wang H, Guo Y, Zhu J, Zhou Y, Zhao H, Liu T. Emodin Suppresses the Migration and Invasion of Melanoma Cells. Biol Pharm Bull. 2021 Jun 1;44(6):771-779. doi: 10.1248/bpb.b20-00807. Epub 2021 Mar 17. PMID: 33731543. 3. Yang S, Zhang Y, Zhang Z, Dan J, Zhou Q, Wang X, Li W, Zhou L, Yang L, Xie L. Insulin Promotes Corneal Nerve Repair and Wound Healing in Type 1 Diabetic Mice by Enhancing Wnt/β-Catenin Signaling. Am J Pathol. 2020 Nov;190(11):2237-2250. doi: 10.1016/j.ajpath.2020.08.006. Epub 2020 Aug 26. PMID: 32858016.
In vitro protocol:	1. Gao H, Yu Z, Li Y, Wang X. miR-100-5p in human umbilical cord mesenchymal stem cell-derived exosomes mediates eosinophilic inflammation to alleviate atherosclerosis via the FZD5/Wnt/β-catenin pathway. Acta Biochim Biophys Sin (Shanghai). 2021 Jul 13:gmab093. doi: 10.1093/abbs/gmab093. Epub ahead of print. PMID: 34254638. 2. Liu C, Chen L, Wang W, Qin D, Jia C, Yuan M, Wang H, Guo Y, Zhu J, Zhou Y, Zhao H, Liu T. Emodin Suppresses the Migration and Invasion of Melanoma Cells. Biol Pharm Bull. 2021 Jun 1;44(6):771-779. doi: 10.1248/bpb.b20-00807. Epub 2021 Mar 17. PMID: 33731543.
In vivo protocol:	1. Yang S, Zhang Y, Zhang Z, Dan J, Zhou Q, Wang X, Li W, Zhou L, Yang L, Xie L. Insulin Promotes Corneal Nerve Repair and Wound Healing in Type 1 Diabetic Mice by Enhancing Wnt/β-Catenin Signaling. Am J Pathol. 2020 Nov;190(11):2237-2250. doi: 10.1016/j.ajpath.2020.08.006. Epub 2020 Aug 26. PMID: 32858016.

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1: Tribulo P, Leão BCDS, Lehloenya KC, Mingoti GZ, Hansen PJ. Consequences of endogenous and exogenous WNT signaling for development of the preimplantation bovine embryo. Biol Reprod. 2017 Jun 1;96(6):1129-1141. doi: 10.1093/biolre/iox048. PubMed PMID: 28575156.

2: Narcisi R, Arikan OH, Lehmann J, Ten Berge D, van Osch GJ. Differential Effects of Small Molecule WNT Agonists on the Multilineage Differentiation Capacity of Human Mesenchymal Stem Cells. Tissue Eng Part A. 2016 Nov;22(21-22):1264-1273. Epub 2016 Oct 17. PubMed PMID: 27633010.

3: Fukuda Y, Sano O, Kazetani K, Yamamoto K, Iwata H, Matsui J. Tubulin is a molecular target of the Wnt-activating chemical probe. BMC Biochem. 2016 May 20;17(1):9. doi: 10.1186/s12858-016-0066-9. PubMed PMID: 27207629; PubMed Central PMCID: PMC4873989.

4: Wang H, Graves MW 2nd, Zhou H, Gu Z, Lamont RJ, Scott DA. 2-Amino-4-(3,4-(methylenedioxy)benzylamino)-6-(3-methoxyphenyl)pyrimidine is an anti-inflammatory TLR-2, -4 and -5 response mediator in human monocytes. Inflamm Res. 2016 Jan;65(1):61-9. doi: 10.1007/s00011-015-0891-0. Epub 2015 Nov 27. PubMed PMID: 26613980; PubMed Central PMCID: PMC4703520.

5: Denicol AC, Dobbs KB, McLean KM, Carambula SF, Loureiro B, Hansen PJ. Canonical WNT signaling regulates development of bovine embryos to the blastocyst stage. Sci Rep. 2013;3:1266. doi: 10.1038/srep01266. Epub 2013 Feb 12. PubMed PMID: 23405280; PubMed Central PMCID: PMC3569626.

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