NBD-Pen
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MedKoo CAT#: 561365

CAS#: 1955505-54-0

Description: NBD-Pen high-sensitivity, specific fluorescence probe for lipid radicals. NBD-Pen directly detected lipid radicals in living cells by turn-on fluorescence. In a rat model of hepatic carcinoma induced by diethylnitrosamine (DEN), NBD-Pen detected lipid radical generation within 1 hour of DEN administration.


Chemical Structure

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NBD-Pen
CAS# 1955505-54-0

Theoretical Analysis

MedKoo Cat#: 561365
Name: NBD-Pen
CAS#: 1955505-54-0
Chemical Formula: C19H30N5O4
Exact Mass: 392.23
Molecular Weight: 392.480
Elemental Analysis: C, 58.15; H, 7.70; N, 17.84; O, 16.31

Price and Availability

Size Price Availability Quantity
10mg USD 450
50mg USD 1650
100mg USD 2650
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Synonym: NBD-Pen; NBD Pen; NBDPen;

IUPAC/Chemical Name: 2,2,6-Trimethyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)-6-pentylpiperidine-1-oxyl

InChi Key: NLURORPZLBRXMF-UHFFFAOYSA-N

InChi Code: InChI=1S/C19H30N5O4/c1-5-6-7-10-19(4)12-14(11-18(2,3)24(19)27)21-17-9-8-15(23(25)26)16-13-20-28-22(16)17/h8-9,13-14,20-21H,5-7,10-12H2,1-4H3

SMILES Code: [O]N1C(C)(C)CC(NC2=CC=C([N+]([O-])=O)C3=CNON32)CC1(C)CCCCC

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Biological target: NBD-Pen high-sensitivity, specific fluorescence probe for lipid radicals.
In vitro activity: To evaluate radical-scavenging activity, this study used electron paramagnetic resonance-spin trapping methods and a fluorescence probe for lipid radicals, NBD-Pen, that this study had developed. Reference: J Am Soc Nephrol. 2020 Feb;31(2):280-296. https://pubmed.ncbi.nlm.nih.gov/31767624/
In vivo activity: This study describes the first high-sensitivity, specific fluorescence probe for lipid radicals, 2,2,6-trimethyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)-6-pentylpiperidine-1-oxyl (NBD-Pen). In a rat model of hepatic carcinoma induced by diethylnitrosamine (DEN), NBD-Pen detected lipid radical generation within 1 h of DEN administration. Reference: Nat Chem Biol. 2016 Aug;12(8):608-13. https://pubmed.ncbi.nlm.nih.gov/27294322/

Preparing Stock Solutions

The following data is based on the product molecular weight 392.48 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Mishima E, Sato E, Ito J, Yamada KI, Suzuki C, Oikawa Y, Matsuhashi T, Kikuchi K, Toyohara T, Suzuki T, Ito S, Nakagawa K, Abe T. Drugs Repurposed as Antiferroptosis Agents Suppress Organ Damage, Including AKI, by Functioning as Lipid Peroxyl Radical Scavengers. J Am Soc Nephrol. 2020 Feb;31(2):280-296. doi: 10.1681/ASN.2019060570. Epub 2019 Nov 25. PMID: 31767624; PMCID: PMC7003311. 2. Ishida Y, Okamoto Y, Matsuoka Y, Tada A, Janprasit J, Yamato M, Morales NP, Yamada KI. Detection and inhibition of lipid-derived radicals in low-density lipoprotein. Free Radic Biol Med. 2017 Dec;113:487-493. doi: 10.1016/j.freeradbiomed.2017.10.388. Epub 2017 Oct 28. PMID: 29107744. 3. Yamada K, Mito F, Matsuoka Y, Ide S, Shikimachi K, Fujiki A, Kusakabe D, Ishida Y, Enoki M, Tada A, Ariyoshi M, Yamasaki T, Yamato M. Fluorescence probes to detect lipid-derived radicals. Nat Chem Biol. 2016 Aug;12(8):608-13. doi: 10.1038/nchembio.2105. Epub 2016 Jun 13. PMID: 27294322.
In vitro protocol: 1. Mishima E, Sato E, Ito J, Yamada KI, Suzuki C, Oikawa Y, Matsuhashi T, Kikuchi K, Toyohara T, Suzuki T, Ito S, Nakagawa K, Abe T. Drugs Repurposed as Antiferroptosis Agents Suppress Organ Damage, Including AKI, by Functioning as Lipid Peroxyl Radical Scavengers. J Am Soc Nephrol. 2020 Feb;31(2):280-296. doi: 10.1681/ASN.2019060570. Epub 2019 Nov 25. PMID: 31767624; PMCID: PMC7003311. 2. Ishida Y, Okamoto Y, Matsuoka Y, Tada A, Janprasit J, Yamato M, Morales NP, Yamada KI. Detection and inhibition of lipid-derived radicals in low-density lipoprotein. Free Radic Biol Med. 2017 Dec;113:487-493. doi: 10.1016/j.freeradbiomed.2017.10.388. Epub 2017 Oct 28. PMID: 29107744.
In vivo protocol: 1. Ishida Y, Okamoto Y, Matsuoka Y, Tada A, Janprasit J, Yamato M, Morales NP, Yamada KI. Detection and inhibition of lipid-derived radicals in low-density lipoprotein. Free Radic Biol Med. 2017 Dec;113:487-493. doi: 10.1016/j.freeradbiomed.2017.10.388. Epub 2017 Oct 28. PMID: 29107744. 2. Yamada K, Mito F, Matsuoka Y, Ide S, Shikimachi K, Fujiki A, Kusakabe D, Ishida Y, Enoki M, Tada A, Ariyoshi M, Yamasaki T, Yamato M. Fluorescence probes to detect lipid-derived radicals. Nat Chem Biol. 2016 Aug;12(8):608-13. doi: 10.1038/nchembio.2105. Epub 2016 Jun 13. PMID: 27294322.

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1: Yamada K, Mito F, Matsuoka Y, Ide S, Shikimachi K, Fujiki A, Kusakabe D, Ishida Y, Enoki M, Tada A, Ariyoshi M, Yamasaki T, Yamato M. Fluorescence probes to detect lipid-derived radicals. Nat Chem Biol. 2016 Aug;12(8):608-13. doi: 10.1038/nchembio.2105. Epub 2016 Jun 13. PubMed PMID: 27294322.