MSAB
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MedKoo CAT#: 561321

CAS#: 173436-66-3

Description: MSAB is a selective inhibitor of Wnt/β-catenin signaling. MSAB shows potent anti-tumor effects selectively on Wnt-dependent cancer cells in vitro and in mouse cancer models. MSAB binds to β-catenin, promoting its degradation, and specifically downregulates Wnt/β-catenin target genes.


Chemical Structure

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MSAB
CAS# 173436-66-3

Theoretical Analysis

MedKoo Cat#: 561321
Name: MSAB
CAS#: 173436-66-3
Chemical Formula: C15H15NO4S
Exact Mass: 305.0722
Molecular Weight: 305.35
Elemental Analysis: C, 59.00; H, 4.95; N, 4.59; O, 20.96; S, 10.50

Price and Availability

Size Price Availability Quantity
50.0mg USD 150.0 Ready to ship
100.0mg USD 250.0 Ready to ship
200.0mg USD 450.0 Ready to ship
500.0mg USD 850.0 Ready to ship
1.0g USD 1450.0 Ready to ship
2.0g USD 2150.0 Ready to ship
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Synonym: MSAB;

IUPAC/Chemical Name: Methyl 3-{[(4-methylphenyl)sulfonyl]amino}benzoate

InChi Key: CVKBYFCJQSPBOI-UHFFFAOYSA-N

InChi Code: InChI=1S/C15H15NO4S/c1-11-6-8-14(9-7-11)21(18,19)16-13-5-3-4-12(10-13)15(17)20-2/h3-10,16H,1-2H3

SMILES Code: O=C(OC)C1=CC=CC(NS(=O)(C2=CC=C(C)C=C2)=O)=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Biological target: MSAB is an inhibitor of Wnt/β-catenin signaling.
In vitro activity: Further, to examine MsaB regulation of ccpE and gudB, this study performed an EMSA, which showed MsaB binding to the ccpE promoter (Figure 5B) and thereby suggested that MsaB regulates the TCA cycle via ccpE. This also confirms previous finding of MsaB regulation of ccpE in the UAMS-1 strain. However, the absence of binding to the promoter of gudB suggests that its regulation by MsaB might be indirect (Figure 5C). All these observations lead this study to conclude that MsaB directly regulates ccpE to control TCA cycle activity and that the increased energy state in the △msaABCR led to decreased persister cell formation in late exponential growth phase. Reference: Front Microbiol. 2021; 12: 657753. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079656/
In vivo activity: Catenin is the mediator of canonical Wnt signalling, and levels of active β-catenin can be reduced using the inhibitor MSAB. This is a cell-permeable compound that binds to β-catenin and induces its ubiquitination and proteasomal degradation. When HCT116 cells were treated with MSAB, there was a reduction in active β-catenin and TNS4 levels were also downregulated (Figure 4A). Reference: Int J Exp Pathol. 2020 Jun;101(3-4):80-86. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370848/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
Soluble in DMSO 25.0 81.9

Preparing Stock Solutions

The following data is based on the product molecular weight 305.35 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Pandey S, Sahukhal GS, Elasri MO. The msaABCR Operon Regulates Persister Formation by Modulating Energy Metabolism in Staphylococcus aureus. Front Microbiol. 2021 Apr 14;12:657753. doi: 10.3389/fmicb.2021.657753. PMID: 33936014; PMCID: PMC8079656. 2. Pal A, Ashworth JC, Collier P, Probert C, Jones S, Leza EP, Meakin ML, A Ritchie A, Onion D, Clarke PA, Allegrucci C, Grabowska AM. A 3D Heterotypic Breast Cancer Model Demonstrates a Role for Mesenchymal Stem Cells in Driving a Proliferative and Invasive Phenotype. Cancers (Basel). 2020 Aug 14;12(8):2290. doi: 10.3390/cancers12082290. PMID: 32824003; PMCID: PMC7465555. 3. Raposo TP, Alfahed A, Nateri AS, Ilyas M. Tensin4 (TNS4) is upregulated by Wnt signalling in adenomas in multiple intestinal neoplasia (Min) mice. Int J Exp Pathol. 2020 Jun;101(3-4):80-86. doi: 10.1111/iep.12352. Epub 2020 Jun 22. PMID: 32567731; PMCID: PMC7370848.
In vitro protocol: 1. Pandey S, Sahukhal GS, Elasri MO. The msaABCR Operon Regulates Persister Formation by Modulating Energy Metabolism in Staphylococcus aureus. Front Microbiol. 2021 Apr 14;12:657753. doi: 10.3389/fmicb.2021.657753. PMID: 33936014; PMCID: PMC8079656. 2. Pal A, Ashworth JC, Collier P, Probert C, Jones S, Leza EP, Meakin ML, A Ritchie A, Onion D, Clarke PA, Allegrucci C, Grabowska AM. A 3D Heterotypic Breast Cancer Model Demonstrates a Role for Mesenchymal Stem Cells in Driving a Proliferative and Invasive Phenotype. Cancers (Basel). 2020 Aug 14;12(8):2290. doi: 10.3390/cancers12082290. PMID: 32824003; PMCID: PMC7465555.
In vivo protocol: 1. Raposo TP, Alfahed A, Nateri AS, Ilyas M. Tensin4 (TNS4) is upregulated by Wnt signalling in adenomas in multiple intestinal neoplasia (Min) mice. Int J Exp Pathol. 2020 Jun;101(3-4):80-86. doi: 10.1111/iep.12352. Epub 2020 Jun 22. PMID: 32567731; PMCID: PMC7370848.

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1: Hwang SY, Deng X, Byun S, Lee C, Lee SJ, Suh H, Zhang J, Kang Q, Zhang T, Westover KD, Mandinova A, Lee SW. Direct Targeting of β-Catenin by a Small Molecule Stimulates Proteasomal Degradation and Suppresses Oncogenic Wnt/β-Catenin Signaling. Cell Rep. 2016 Jun 28;16(1):28-36. doi: 10.1016/j.celrep.2016.05.071. Epub 2016 Jun 16. PubMed PMID: 27320923; PubMed Central PMCID: PMC4957947.

MSAB

50.0mg / USD 150.0