OUL35
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MedKoo CAT#: 561162

CAS#: 6336-34-1

Description: OUL35, also known as NSC39047, is a selective PARP-10 inhibitor, and small-molecule ARTD10 inhibitor. OUL35 has been shown to rescue cells from ARTD10-induced cell death.

Chemical Structure

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OUL35
CAS# 6336-34-1
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 561162
Name: OUL35
CAS#: 6336-34-1
Chemical Formula: C14H12N2O3
Exact Mass: 256.08
Molecular Weight: 256.261
Elemental Analysis: C, 65.62; H, 4.72; N, 10.93; O, 18.73

Price and Availability

Size Price Availability Quantity
50mg USD 250 2 Weeks
100mg USD 450 2 Weeks
200mg USD 750 2 Weeks
500mg USD 1450 2 Weeks
1g USD 2450 2 Weeks
2g USD 4650 2 Weeks
5g USD 6950 2 Weeks
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Synonym: OUL35; OUL 35; OUL-35; NSC39047; NSC 39047; NSC-39047

IUPAC/Chemical Name: 4-(4-Carbamoylphenoxy)benzamide

InChi Key: XZRCQWLPMXFGHE-UHFFFAOYSA-N

InChi Code: InChI=1S/C14H12N2O3/c15-13(17)9-1-5-11(6-2-9)19-12-7-3-10(4-8-12)14(16)18/h1-8H,(H2,15,17)(H2,16,18)

SMILES Code: O=C(N)C1=CC=C(OC2=CC=C(C(N)=O)C=C2)C=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Instruction:
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Biological target: OUL35 (NSC39047) is a potent and selective inhibitor of ARTD10 (PARP-10), with an IC50 of 329 nM.
In vitro activity: The treatment of HeLa cells overexpressing ARTD10 with OUL35 resulted in a complete rescue of cell proliferation. Notably, the inactive analogs OUL77 and OUL78 were ineffective and did not facilitate colony formation (Figures 3A and 3B). Together, these findings provide evidence that OUL35 enters cells and inhibits the catalytic activity of ARTD10 in a dose-dependent manner with an IC50 of 1.35 μM (Figure 3C). To further substantiate this, this study performed cellular thermal shift assays (CETSA) showing that endogenous ARTD10 in U2OS cells is stabilized by OUL35 (Figure 3D). Reference: Cell Chem Biol. 2016 Oct 20;23(10):1251-1260. https://pubmed.ncbi.nlm.nih.gov/27667561/
In vivo activity: TBD

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 5.0 19.51
DMSO 46.3 180.75
DMSO:PBS (pH 7.2) (1:4) 0.2 0.78

Preparing Stock Solutions

The following data is based on the product molecular weight 256.26 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Tian Y, Korn P, Tripathi P, Komnig D, Wiemuth D, Nikouee A, Classen A, Bolm C, Falkenburger BH, Lüscher B, Gründer S. The mono-ADP-ribosyltransferase ARTD10 regulates the voltage-gated K+ channel Kv1.1 through protein kinase C delta. BMC Biol. 2020 Oct 15;18(1):143. doi: 10.1186/s12915-020-00878-1. PMID: 33059680; PMCID: PMC7558731. 2. Venkannagari H, Verheugd P, Koivunen J, Haikarainen T, Obaji E, Ashok Y, Narwal M, Pihlajaniemi T, Lüscher B, Lehtiö L. Small-Molecule Chemical Probe Rescues Cells from Mono-ADP-Ribosyltransferase ARTD10/PARP10-Induced Apoptosis and Sensitizes Cancer Cells to DNA Damage. Cell Chem Biol. 2016 Oct 20;23(10):1251-1260. doi: 10.1016/j.chembiol.2016.08.012. Epub 2016 Sep 22. PMID: 27667561.
In vitro protocol: 1. Tian Y, Korn P, Tripathi P, Komnig D, Wiemuth D, Nikouee A, Classen A, Bolm C, Falkenburger BH, Lüscher B, Gründer S. The mono-ADP-ribosyltransferase ARTD10 regulates the voltage-gated K+ channel Kv1.1 through protein kinase C delta. BMC Biol. 2020 Oct 15;18(1):143. doi: 10.1186/s12915-020-00878-1. PMID: 33059680; PMCID: PMC7558731. 2. Venkannagari H, Verheugd P, Koivunen J, Haikarainen T, Obaji E, Ashok Y, Narwal M, Pihlajaniemi T, Lüscher B, Lehtiö L. Small-Molecule Chemical Probe Rescues Cells from Mono-ADP-Ribosyltransferase ARTD10/PARP10-Induced Apoptosis and Sensitizes Cancer Cells to DNA Damage. Cell Chem Biol. 2016 Oct 20;23(10):1251-1260. doi: 10.1016/j.chembiol.2016.08.012. Epub 2016 Sep 22. PMID: 27667561.
In vivo protocol: TBD

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1: Venkannagari H, Verheugd P, Koivunen J, Haikarainen T, Obaji E, Ashok Y, Narwal M, Pihlajaniemi T, Lüscher B, Lehtiö L. Small-Molecule Chemical Probe Rescues Cells from Mono-ADP-Ribosyltransferase ARTD10/PARP10-Induced Apoptosis and Sensitizes Cancer Cells to DNA Damage. Cell Chem Biol. 2016 Oct 20;23(10):1251-1260. doi: 10.1016/j.chembiol.2016.08.012. Epub 2016 Sep 22. PubMed PMID: 27667561.