WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 525605

CAS#: 1051933-86-8

Description: M199 is a novel inhibitor of TLR3/9 activation, inducing secretion of IL-6, IL-8 and TNFalpha in human PBMCs.

Chemical Structure

CAS# 1051933-86-8

Theoretical Analysis

MedKoo Cat#: 525605
Name: M199
CAS#: 1051933-86-8
Chemical Formula: C17H17N3O
Exact Mass: 279.1372
Molecular Weight: 279.343
Elemental Analysis: C, 73.10; H, 6.13; N, 15.04; O, 5.73

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

Request quote for custom synthesis

Synonym: M199

IUPAC/Chemical Name: 1-Ethyl-3-(6-methyl-phenanthridin-8-yl)-urea


InChi Code: InChI=1S/C17H17N3O/c1-3-18-17(21)20-12-8-9-13-14-6-4-5-7-16(14)19-11(2)15(13)10-12/h4-10H,3H2,1-2H3,(H2,18,20,21)


Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 279.343 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Dilution Calculator

Calculate the dilution required to prepare a stock solution.

1: Talepoor AG, Kalani M, Dahaghani AS, Doroudchi M. Hydrogen Peroxide and Lipopolysaccharide Differentially Affect the Expression of MicroRNAs 10a, 33a, 21, 221 in Endothelial Cells Before and After Coculture With Monocytes. Int J Toxicol. 2017 Mar/Apr;36(2):133-141. doi: 10.1177/1091581817695270. PubMed PMID: 28403739.

2: Yao Q, Song R, Ao L, Cleveland JC Jr, Fullerton DA, Meng X. Neurotrophin 3 up-regulates proliferation and collagen production in human aortic valve interstitial cells: a potential role in aortic valve sclerosis. Am J Physiol Cell Physiol. 2017 Mar 29:ajpcell.00292.2016. doi: 10.1152/ajpcell.00292.2016. [Epub ahead of print] PubMed PMID: 28356268.

3: Teofilović NK, Bihi M, Stojković MR, Tumir LM, Ester K, Kralj M, Majhen D, Oršolić N, Lepur A, Vrbanec D, Markotić A, Dembić Z, Weber AN, Piantanida I, Vugrek O, Diken M, Knežević J. 1-ethyl-3-(6-methylphenanthridine-8-il) urea modulates TLR3/9 activation and induces selective pro-inflammatory cytokine expression in vitro. Bioorg Med Chem Lett. 2017 Apr 1;27(7):1530-1537. doi: 10.1016/j.bmcl.2017.02.048. Epub 2017 Feb 20. PubMed PMID: 28254484.

4: Canesin HS, Brom-de-Luna JG, Choi YH, Ortiz I, Diaw M, Hinrichs K. Blastocyst development after intracytoplasmic sperm injection of equine oocytes vitrified at the germinal-vesicle stage. Cryobiology. 2017 Apr;75:52-59. doi: 10.1016/j.cryobiol.2017.02.004. Epub 2017 Feb 13. PubMed PMID: 28209499.

5: Rojas-Martínez C, Rodríguez-Vivas RI, Figueroa Millán JV, Acosta Viana KY, Gutiérrez Ruiz EJ, Álvarez Martínez JA. Putrescine: Essential factor for in vitro proliferation of Babesia bovis. Exp Parasitol. 2017 Apr;175:79-84. doi: 10.1016/j.exppara.2017.01.010. Epub 2017 Jan 30. PubMed PMID: 28153804.

6: Jing H, Lin X, Xu L, Gao L, Zhang M, Wang N, Wu S. Establishment and characterization of a heart-derived cell line from goldfish (Carassius auratus). Fish Physiol Biochem. 2017 Jan 18. doi: 10.1007/s10695-017-0345-4. [Epub ahead of print] PubMed PMID: 28097494.

7: Klos M, Mundada L, Banerjee I, Morgenstern S, Myers S, Leone M, Kleid M, Herron T, Devaney E. Altered myocyte contractility and calcium homeostasis in alpha-myosin heavy chain point mutations linked to familial dilated cardiomyopathy. Arch Biochem Biophys. 2017 Feb 1;615:53-60. doi: 10.1016/j.abb.2016.12.007. Epub 2017 Jan 11. PubMed PMID: 28088328.

8: Luther I, Jakop U, Lueders I, Tordiffe A, Franz C, Schiller J, Kotze A, Müller K. Semen cryopreservation and radical reduction capacity of seminal fluid in captive African lion (Panthera leo). Theriogenology. 2017 Feb;89:295-304. doi: 10.1016/j.theriogenology.2016.10.024. Epub 2016 Nov 9. PubMed PMID: 28043366.

9: Kuma A, Tamura M, Ishimatsu N, Harada Y, Izumi H, Miyamoto T, Furuno Y, Nakano Y, Serino R, Otsuji Y. Monocarboxylate Transporter-1 Mediates the Protective Effects of Neutral-pH Bicarbonate/Lactate-Buffered Peritoneal Dialysis Fluid on Cell Viability and Apoptosis. Ther Apher Dial. 2017 Feb;21(1):62-70. doi: 10.1111/1744-9987.12476. Epub 2016 Dec 12. PubMed PMID: 27957817.

10: Dzialoszynski TM, Milne KJ, Trevithick JR, Noble EG. Heat shock protein concentration and clarity of porcine lenses incubated at elevated temperatures. Mol Vis. 2016 Oct 31;22:1309-1317. eCollection 2016. PubMed PMID: 27843266; PubMed Central PMCID: PMC5087965.

11: Taiyab A, Korol A, Deschamps PA, West-Mays JA. β-Catenin/CBP-Dependent Signaling Regulates TGF-β-Induced Epithelial to Mesenchymal Transition of Lens Epithelial Cells. Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5736-5747. doi: 10.1167/iovs.16-20162. PubMed PMID: 27787561; PubMed Central PMCID: PMC5089212.

12: Pratti JE, Ramos TD, Pereira JC, da Fonseca-Martins AM, Maciel-Oliveira D, Oliveira-Silva G, de Mello MF, Chaves SP, Gomes DC, Diaz BL, Rossi-Bergmann B, de Matos Guedes HL. Efficacy of intranasal LaAg vaccine against Leishmania amazonensis infection in partially resistant C57Bl/6 mice. Parasit Vectors. 2016 Oct 6;9(1):534. PubMed PMID: 27716449; PubMed Central PMCID: PMC5052793.

13: Khan S, Villalobos MA, Choron RL, Chang S, Brown SA, Carpenter JP, Tulenko TN, Zhang P. Fibroblast growth factor and vascular endothelial growth factor play a critical role in endotheliogenesis from human adipose-derived stem cells. J Vasc Surg. 2017 May;65(5):1483-1492. doi: 10.1016/j.jvs.2016.04.034. Epub 2016 Aug 8. PubMed PMID: 27514438.

14: Degani G, Ragni E, Botias P, Ravasio D, Calderon J, Pianezzola E, Rodriguez-Peña JM, Vanoni MA, Arroyo J, Fonzi WA, Popolo L. Genomic and functional analyses unveil the response to hyphal wall stress in Candida albicans cells lacking β(1,3)-glucan remodeling. BMC Genomics. 2016 Jul 2;17:482. doi: 10.1186/s12864-016-2853-5. PubMed PMID: 27411447; PubMed Central PMCID: PMC4942948.

15: Ng HH, Leo CH, Parry LJ. Serelaxin (recombinant human relaxin-2) prevents high glucose-induced endothelial dysfunction by ameliorating prostacyclin production in the mouse aorta. Pharmacol Res. 2016 May;107:220-8. doi: 10.1016/j.phrs.2016.03.011. Epub 2016 Mar 16. PubMed PMID: 26993102.

16: Ferré LB, Bogliotti Y, Chitwood JL, Fresno C, Ortega HH, Kjelland ME, Ross PJ. Effect of spermatozoa motility hyperactivation factors and gamete coincubation duration on in vitro bovine embryo development using flow cytometrically sorted spermatozoa. Reprod Fertil Dev. 2016 Feb 24. doi: 10.1071/RD15289. [Epub ahead of print] PubMed PMID: 26907580.

17: Li WP, Cui XD, Hou NN, Zhang XY, Liu JH, Zhang J, Cheng M. [Effects of inward rectifier potassium channel blockers on EPCs function]. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2015 Sep;31(5):448-51. Chinese. PubMed PMID: 26827539.

18: Wang W, Said A, Wang Y, Fu Q, Xiao Y, Lv S, Shen Z. Establishment and characterization of duck embryo epithelial (DEE) cell line and its use as a new approach toward DHAV-1 propagation and vaccine development. Virus Res. 2016 Feb 2;213:260-8. doi: 10.1016/j.virusres.2015.12.021. Epub 2015 Dec 29. PubMed PMID: 26739426.

19: López-Díaz MC, Buján-Varela J, Cadórniga-Valiño C. Viable pluripotent chick blastodermal cells can be maintained long term in an alkaline defined medium. In Vitro Cell Dev Biol Anim. 2016 Apr;52(4):385-94. doi: 10.1007/s11626-015-9989-5. Epub 2015 Dec 31. PubMed PMID: 26721239.

20: Nkadimeng M, van Marle-Koster E, Netshirovha TR, Nedambale TL, Lehloenya KC. 145 THE ROLE OF CELL APOPTOSIS ON IN VITRO-PRODUCED BEEF CATTLE EMBRYOS. Reprod Fertil Dev. 2016 Jan;29(1):181. doi: 10.1071/RDv29n1Ab145. PubMed PMID: 28278942.