ML241 HCl
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MedKoo CAT#: 406700

CAS#: 2070015-13-1 (HCl)

Description: ML241 is a potent and selective inhibitors of p97 ATPase. ML241 inhibit p97 ATPase with IC(50) values of 100 nM. ML241 inhibits degradation of a p97-dependent but not a p97-independent proteasome substrate in a dual-reporter cell line. ML241 may be a novel agent for the chemotherapy of cancer, and provide a rationale for developing pathway-specific p97 inhibitors.


Chemical Structure

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ML241 HCl
CAS# 2070015-13-1 (HCl)

Theoretical Analysis

MedKoo Cat#: 406700
Name: ML241 HCl
CAS#: 2070015-13-1 (HCl)
Chemical Formula: C23H25ClN4O
Exact Mass: 0.00
Molecular Weight: 408.930
Elemental Analysis: C, 67.56; H, 6.16; Cl, 8.67; N, 13.70; O, 3.91

Price and Availability

Size Price Availability Quantity
10mg USD 110 Ready to ship
25mg USD 220 Ready to ship
50mg USD 385 Ready to ship
100mg USD 685 Ready to ship
200mg USD 1250 Ready to ship
500mg USD 1950 Ready to ship
1g USD 2850 2 weeks
2g USD 4250 2 weeks
5g USD 7450 2 weeks
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Related CAS #: 2070015-13-1 (HCl)   1346528-06-0 (free base)    

Synonym: ML241; ML-241; ML 241; ML241 HCl; ML241 hydrochloride

IUPAC/Chemical Name: 2-(2H-benzo[b][1,4]oxazin-4(3H)-yl)-N-benzyl-5,6,7,8-tetrahydroquinazolin-4-amine hydrochloride

InChi Key: DYHMHNNBOLCULH-UHFFFAOYSA-N

InChi Code: InChI=1S/C23H24N4O.ClH/c1-2-8-17(9-3-1)16-24-22-18-10-4-5-11-19(18)25-23(26-22)27-14-15-28-21-13-7-6-12-20(21)27;/h1-3,6-9,12-13H,4-5,10-11,14-16H2,(H,24,25,26);1H

SMILES Code: [H]Cl.C1(N2C3=CC=CC=C3OCC2)=NC(NCC4=CC=CC=C4)=C5CCCCC5=N1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target: ML241 hydrochloride is a potent p97 inhibitor, inhibiting p97 ATPase with IC50 value of 100 nM.
In vitro activity: Consistent with the role of p97 in the ubiquitin–proteasome system (UPS), ML240 and ML241, like the parent compound DBeQ, caused accumulation of ubiquitin conjugates in the nuclear plus membrane and cytosolic compartments at concentrations of 5–10 μm (Figure 4a). Interestingly, ML241 caused stronger accumulation than ML240, even though ML240 was more potent at stabilizing UbG76V–GFP. This, along with the cell proliferation data, points to unexpected complexity in the mechanism of action of these compounds. Reference: ChemMedChem. 2013 Feb; 8(2): 297–312. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662613/
In vivo activity: N/A

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 54.0 132.05
Ethanol 20.0 48.91

Preparing Stock Solutions

The following data is based on the product molecular weight 408.93 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Chou TF, Li K, Frankowski KJ, Schoenen FJ, Deshaies RJ. Structure-activity relationship study reveals ML240 and ML241 as potent and selective inhibitors of p97 ATPase. ChemMedChem. 2013 Feb;8(2):297-312. doi: 10.1002/cmdc.201200520. Epub 2013 Jan 11. PMID: 23316025; PMCID: PMC3662613. 2. Fang CJ, Gui L, Zhang X, Moen DR, Li K, Frankowski KJ, Lin HJ, Schoenen FJ, Chou TF. Evaluating p97 inhibitor analogues for their domain selectivity and potency against the p97-p47 complex. ChemMedChem. 2015 Jan;10(1):52-6. doi: 10.1002/cmdc.201402420. Epub 2014 Nov 6. PMID: 25377500; PMCID: PMC4280364.
In vitro protocol: 1. Chou TF, Li K, Frankowski KJ, Schoenen FJ, Deshaies RJ. Structure-activity relationship study reveals ML240 and ML241 as potent and selective inhibitors of p97 ATPase. ChemMedChem. 2013 Feb;8(2):297-312. doi: 10.1002/cmdc.201200520. Epub 2013 Jan 11. PMID: 23316025; PMCID: PMC3662613. 2. Fang CJ, Gui L, Zhang X, Moen DR, Li K, Frankowski KJ, Lin HJ, Schoenen FJ, Chou TF. Evaluating p97 inhibitor analogues for their domain selectivity and potency against the p97-p47 complex. ChemMedChem. 2015 Jan;10(1):52-6. doi: 10.1002/cmdc.201402420. Epub 2014 Nov 6. PMID: 25377500; PMCID: PMC4280364.
In vivo protocol: N/A

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1: Fang CJ, Gui L, Zhang X, Moen DR, Li K, Frankowski KJ, Lin HJ, Schoenen FJ, Chou TF. Evaluating p97 Inhibitor Analogues for Their Domain Selectivity and Potency against the p97-p47 Complex. ChemMedChem. 2015 Jan;10(1):52-6. doi: 10.1002/cmdc.201402420. Epub 2014 Nov 6. PubMed PMID: 25377500; PubMed Central PMCID: PMC4280364.

2: Chou TF, Bulfer SL, Weihl CC, Li K, Lis LG, Walters MA, Schoenen FJ, Lin HJ, Deshaies RJ, Arkin MR. Specific inhibition of p97/VCP ATPase and kinetic analysis demonstrate interaction between D1 and D2 ATPase domains. J Mol Biol. 2014 Jul 29;426(15):2886-99. doi: 10.1016/j.jmb.2014.05.022. Epub 2014 May 27. PubMed PMID: 24878061; PubMed Central PMCID: PMC4102644.

3: Chou TF, Li K, Nordin BE, Porubsky P, Frankowski K, Patricelli MP, Aubé J, Schoenen FJ, Deshaies R. Selective, reversible inhibitors of the AAA ATPase p97. 2011 Apr 14 [updated 2013 May 01]. Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010-. Available from http://www.ncbi.nlm.nih.gov/books/NBK133422/ PubMed PMID: 23658965.

4: Chou TF, Li K, Frankowski KJ, Schoenen FJ, Deshaies RJ. Structure-activity relationship study reveals ML240 and ML241 as potent and selective inhibitors of p97 ATPase. ChemMedChem. 2013 Feb;8(2):297-312. doi: 10.1002/cmdc.201200520. Epub 2013 Jan 11. PubMed PMID: 23316025; PubMed Central PMCID: PMC3662613.