WARNING: This product is for research use only, not for human or veterinary use.
MedKoo CAT#: 329511
CAS#: 1809249-37-3 (free base)
Description: Remdesivir, also known as GS-5734, is a prodrug form of the antiviral nucleoside analog GS-44152. Upon entry into cells, remdesivir is metabolized into the nucleotide triphosphate GS-441524. Remdesivir inhibits murine hepatitis virus (MHV) with an EC50 of 30 nM, and blocks SARS-CoV and MERS-CoV in HAE cells with EC50s of both 74 nM in HAE cells after treatment for 24 h. GS-5734 inhibits both epidemic and zoonotic coronaviruses. It was developed as a treatment for filovirus infections such as Ebola virus disease and Marburg virus. Remdesivir was approved for treatment of COVID-19. ******* WARNING****** Our product Remdesivir is a Pure chemical in Powder form for laboratory research use only, NOT FOR HUMAN OR PATIENT USE
MedKoo Cat#: 329511
Name: Remdesivir
CAS#: 1809249-37-3 (free base)
Chemical Formula: C27H35N6O8P
Exact Mass: 602.2254
Molecular Weight: 602.58
Elemental Analysis: C, 53.82; H, 5.85; N, 13.95; O, 21.24; P, 5.14
Related CAS #: 1809249-37-3 (free base) 2250110-53-1 (maleate)
Synonym: Remdesivir; GS-5734; GS 5734; GS5734, Prodrug of GS-441524; Prodrug of GS441524; Prodrug of GS441524;
IUPAC/Chemical Name: 2-ethylbutyl ((S)-(((2R,3S,4R,5R)-5-(4-aminopyrrolo[2,1-f][1,2,4]triazin-7-yl)-5-cyano-3,4-dihydroxytetrahydrofuran-2-yl)methoxy)(phenoxy)phosphoryl)-L-alaninate
InChi Key: RWWYLEGWBNMMLJ-YSOARWBDSA-N
InChi Code: InChI=1S/C27H35N6O8P/c1-4-18(5-2)13-38-26(36)17(3)32-42(37,41-19-9-7-6-8-10-19)39-14-21-23(34)24(35)27(15-28,40-21)22-12-11-20-25(29)30-16-31-33(20)22/h6-12,16-18,21,23-24,34-35H,4-5,13-14H2,1-3H3,(H,32,37)(H2,29,30,31)/t17-,21+,23+,24+,27-,42-/m0/s1
SMILES Code: C[C@H](N[P@@](OC1=CC=CC=C1)(OC[C@H]2O[C@@](C#N)(C3=CC=C4C(N)=NC=NN43)[C@H](O)[C@@H]2O)=O)C(OCC(CC)CC)=O
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
| Biological target: | Nucleoside analogue with effective antiviral activity and is highly effective in the control of SARS-CoV-2 (COVID-19) infection in vitro. |
| In vitro activity: | In vitro, Remdesivir (RDV) exhibited antiviral activity against a clinical isolate of SARS-CoV-2 in primary human airway epithelial cells with a half-maximal effective concentration of 9.9 nM and also potently (280 nM) inhibited SARS-CoV-2 replication in Calu-3 human lung cells. In biochemical assays assessing RDV-triphosphate incorporation by the SARS-CoV-2, SARS-CoV, and MERS-CoV viral RNA-dependent RNA polymerase complexes, RDV triphosphate was selectively incorporated over the natural nucleotide substrate adenosine triphosphate and inhibited viral RNA synthesis with a half-maximal inhibitory concentration value of 32 nM for MERS-CoV. Reference: Clin Pharmacokinet. 2021 Mar 30 : 1–15. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007387/ |
| In vivo activity: | In vivo, Remdesivir (RDV) showed therapeutic efficacy in SARS-CoV-2-infected rhesus monkeys and prophylactic and therapeutic efficacy in MERS-CoV-infected rhesus monkeys. Briefly, 12 h after inoculation with SARS-CoV-2, rhesus monkeys received an RDV 10-mg/kg IV loading dose followed by maintenance doses of RDV 5 mg/kg at 24 h post-inoculation and once daily thereafter for a total of 6 days of treatment. The aim of the loading dose was to rapidly generate high GS-443902 concentrations following the first dose. Treatment with this regimen resulted in a significant reduction in clinical signs of respiratory disease, lung pathology and gross lung lesions, and viral RNA levels compared with vehicle-treated animals. Reference: Clin Pharmacokinet. 2021 Mar 30 : 1–15. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8007387/ |
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 100.0 | 165.95 | |
| Ethanol | 12.0 | 20.0 |
The following data is based on the product molecular weight 602.58 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Option 1: | (1). Formulation for in vivo study (Author from NIH): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104368/ ....treated with 5 mg/kg remdesivir in vehicle solution (5 mg/mL 12% sulfobutylether-β-cyclodextrin in water and hydrochloric acid, pH3.5) |
| Option 2: | (2). Formulation for in vivo study ( Author from CDC) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732787/ One group of animals received remdesivir (10 mg/kg) (Gilead Sciences) in vehicle solution [12% sulfobutylether-β-cyclodextrin in water and hydrochloric acid (pH 3.5)] |
| Formulation protocol: | 1. Humeniuk R, Mathias A, Kirby BJ, Lutz JD, Cao H, Osinusi A, Babusis D, Porter D, Wei X, Ling J, Reddy YS, German P. Pharmacokinetic, Pharmacodynamic, and Drug-Interaction Profile of Remdesivir, a SARS-CoV-2 Replication Inhibitor. Clin Pharmacokinet. 2021 Mar 30:1–15. doi: 10.1007/s40262-021-00984-5. Epub ahead of print. PMID: 33782830; PMCID: PMC8007387. 2. Buckland MS, Galloway JB, Fhogartaigh CN, Meredith L, Provine NM, Bloor S, Ogbe A, Zelek WM, Smielewska A, Yakovleva A, Mann T, Bergamaschi L, Turner L, Mescia F, Toonen EJM, Hackstein CP, Akther HD, Vieira VA, Ceron-Gutierrez L, Periselneris J, Kiani-Alikhan S, Grigoriadou S, Vaghela D, Lear SE, Török ME, Hamilton WL, Stockton J, Quick J, Nelson P, Hunter M, Coulter TI, Devlin L; CITIID-NIHR COVID-19 BioResource Collaboration; MRC-Toxicology Unit COVID-19 Consortium, Bradley JR, Smith KGC, Ouwehand WH, Estcourt L, Harvala H, Roberts DJ, Wilkinson IB, Screaton N, Loman N, Doffinger R, Lyons PA, Morgan BP, Goodfellow IG, Klenerman P, Lehner PJ, Matheson NJ, Thaventhiran JED. Treatment of COVID-19 with remdesivir in the absence of humoral immunity: a case report. Nat Commun. 2020 Dec 14;11(1):6385. doi: 10.1038/s41467-020-19761-2. PMID: 33318491; PMCID: PMC7736571. |
| In vitro protocol: | 1. Humeniuk R, Mathias A, Kirby BJ, Lutz JD, Cao H, Osinusi A, Babusis D, Porter D, Wei X, Ling J, Reddy YS, German P. Pharmacokinetic, Pharmacodynamic, and Drug-Interaction Profile of Remdesivir, a SARS-CoV-2 Replication Inhibitor. Clin Pharmacokinet. 2021 Mar 30:1–15. doi: 10.1007/s40262-021-00984-5. Epub ahead of print. PMID: 33782830; PMCID: PMC8007387. |
| In vivo protocol: | 1. Humeniuk R, Mathias A, Kirby BJ, Lutz JD, Cao H, Osinusi A, Babusis D, Porter D, Wei X, Ling J, Reddy YS, German P. Pharmacokinetic, Pharmacodynamic, and Drug-Interaction Profile of Remdesivir, a SARS-CoV-2 Replication Inhibitor. Clin Pharmacokinet. 2021 Mar 30:1–15. doi: 10.1007/s40262-021-00984-5. Epub ahead of print. PMID: 33782830; PMCID: PMC8007387. 2. Buckland MS, Galloway JB, Fhogartaigh CN, Meredith L, Provine NM, Bloor S, Ogbe A, Zelek WM, Smielewska A, Yakovleva A, Mann T, Bergamaschi L, Turner L, Mescia F, Toonen EJM, Hackstein CP, Akther HD, Vieira VA, Ceron-Gutierrez L, Periselneris J, Kiani-Alikhan S, Grigoriadou S, Vaghela D, Lear SE, Török ME, Hamilton WL, Stockton J, Quick J, Nelson P, Hunter M, Coulter TI, Devlin L; CITIID-NIHR COVID-19 BioResource Collaboration; MRC-Toxicology Unit COVID-19 Consortium, Bradley JR, Smith KGC, Ouwehand WH, Estcourt L, Harvala H, Roberts DJ, Wilkinson IB, Screaton N, Loman N, Doffinger R, Lyons PA, Morgan BP, Goodfellow IG, Klenerman P, Lehner PJ, Matheson NJ, Thaventhiran JED. Treatment of COVID-19 with remdesivir in the absence of humoral immunity: a case report. Nat Commun. 2020 Dec 14;11(1):6385. doi: 10.1038/s41467-020-19761-2. PMID: 33318491; PMCID: PMC7736571. |
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(1). formulation for in vivo study (Author from NIH):
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7104368/
....treated with 5 mg/kg remdesivir in vehicle solution (5 mg/mL 12% sulfobutylether-β-cyclodextrin in water and hydrochloric acid, pH3.5)
(2). formulation for in vivo study ( Author from CDC)
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732787/
One group of animals received remdesivir (10 mg/kg) (Gilead Sciences) in vehicle solution [12% sulfobutylether-β-cyclodextrin in water and hydrochloric acid (pH 3.5)]
