WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 406971

CAS#: 5142-23-4

Description: 3-Methyladenine, aslo known as NSC 66389, is an Autophagy inhibitor. 3-methyladenine enhances the sensitivity of nasopharyngeal carcinoma cells to chemotherapy and radiotherapy. 3-Methyladenine can depress drug efflux transporters via blocking the PI3K-AKT-mTOR pathway thus sensitizing MDR cancer to chemotherapy. 3-methyladenine potentiates apoptosis induced by dietary tocotrienols in breast cancer cells.

Price and Availability


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3-Methyladenine, purity > 98%, is in stock. Current shipping out time is about 2 weeks after order is received. CoA, QC data and MSDS documents are available in one week after order is received.

Chemical Structure


Theoretical Analysis

MedKoo Cat#: 406971
Name: 3-Methyladenine
CAS#: 5142-23-4
Chemical Formula: C6H7N5
Exact Mass: 149.0701
Molecular Weight: 149.157
Elemental Analysis: C, 48.32; H, 4.73; N, 46.95

Synonym: 3-Methyladenine, NSC 66389; NSC-66389; NSC66389.

IUPAC/Chemical Name: 3-methyl-3H-purin-6-amine


InChi Code: InChI=1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3


Technical Data

Solid powder

>98% (or refer to the Certificate of Analysis)

Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition:
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Soluble in DMSO

Shelf Life:
>2 years if stored properly

Drug Formulation:
This drug may be formulated in DMSO

Stock Solution Storage:
0 - 4 C for short term (days to weeks), or -20 C for long term (months).

Harmonized System Code:


1: Zheng XY, Li LJ, Li W, Jiang PF, Shen HQ, Chen YH, Chen X. Low concentrations of chloroquine and 3-methyladenine suppress the viability of retinoblastoma cells synergistically with vincristine independent of autophagy inhibition. Graefes Arch Clin Exp Ophthalmol. 2015 Dec;253(12):2309-15. doi: 10.1007/s00417-015-3157-1. PubMed PMID: 26335535.

2: Song L, Ma L, Chen G, Huang Y, Sun X, Jiang C, Liu H. [Autophagy inhibitor 3-methyladenine enhances the sensitivity of nasopharyngeal carcinoma cells to chemotherapy and radiotherapy]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2016 Jan;41(1):9-18. doi: 10.11817/j.issn.1672-7347.2016.01.002. Chinese. PubMed PMID: 26819419.

3: Hermann R, Vélez DE, Rusiecki TM, Fernández Pazos Mde L, Mestre Cordero VE, Marina Prendes MG, Perazzo Rossini JC, Savino EA, Varela A. Effects of 3-methyladenine on isolated left atria subjected to simulated ischaemia-reperfusion. Clin Exp Pharmacol Physiol. 2015 Jan;42(1):41-51. doi: 10.1111/1440-1681.12323. PubMed PMID: 25311855.

4: Zou Z, Zhang J, Zhang H, Liu H, Li Z, Cheng D, Chen J, Liu L, Ni M, Zhang Y, Yao J, Zhou J, Fu J, Liang Y. 3-Methyladenine can depress drug efflux transporters via blocking the PI3K-AKT-mTOR pathway thus sensitizing MDR cancer to chemotherapy. J Drug Target. 2014 Nov;22(9):839-48. doi: 10.3109/1061186X.2014.936870. PubMed PMID: 25019701.

5: Zhang R, Wang R, Chen Q, Chang H. Inhibition of autophagy using 3-methyladenine increases cisplatin-induced apoptosis by increasing endoplasmic reticulum stress in U251 human glioma cells. Mol Med Rep. 2015 Aug;12(2):1727-32. doi: 10.3892/mmr.2015.3588. PubMed PMID: 25846607; PubMed Central PMCID: PMC4464427.

6: Horwacik I, Gaik M, Durbas M, Boratyn E, Zając G, Szychowska K, Szczodrak M, Kołoczek H, Rokita H. Inhibition of autophagy by 3-methyladenine potentiates sulforaphane-induced cell death of BE(2)-C human neuroblastoma cells. Mol Med Rep. 2015 Jul;12(1):535-42. doi: 10.3892/mmr.2015.3377. PubMed PMID: 25695841.

7: Pickard RD, Spencer BH, McFarland AJ, Bernaitis N, Davey AK, Perkins AV, Chess-Williams R, McDermott CM, Forbes A, Christie D, Anoopkumar-Dukie S. Paradoxical effects of the autophagy inhibitor 3-methyladenine on docetaxel-induced toxicity in PC-3 and LNCaP prostate cancer cells. Naunyn Schmiedebergs Arch Pharmacol. 2015 Jul;388(7):793-9. doi: 10.1007/s00210-015-1104-7. PubMed PMID: 25708950.

8: Alam MJ, Ahmad S. Quantum chemical and spectroscopic investigations of 3-methyladenine. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jul 15;128:653-64. doi: 10.1016/j.saa.2014.02.170. PubMed PMID: 24704482.

9: Liu J, Hao G, Yi L, Sun TS. [Protective effects of 3-methyladenine on acute lung injury caused by multiple trauma in rats]. Zhongguo Gu Shang. 2015 Apr;28(4):350-3. Chinese. PubMed PMID: 26072619.

10: Tran AT, Ramalinga M, Kedir H, Clarke R, Kumar D. Autophagy inhibitor 3-methyladenine potentiates apoptosis induced by dietary tocotrienols in breast cancer cells. Eur J Nutr. 2015 Mar;54(2):265-72. doi: 10.1007/s00394-014-0707-y. PubMed PMID: 24830781; PubMed Central PMCID: PMC4233202.

11: Zhu B, Yang C, Ding LC, Liu N. 3-methyladenine, an autophagic inhibitor, attenuates therapeutic effects of sirolimus on scopolamine-induced cognitive dysfunction in a rat model. Int J Clin Exp Med. 2014 Oct 15;7(10):3327-32. PubMed PMID: 25419365; PubMed Central PMCID: PMC4238551.

12: McFarland AJ, Grant GD, Perkins AV, Flegg C, Davey AK, Allsopp TJ, Renshaw G, Kavanagh J, McDermott CM, Anoopkumar-Dukie S. Paradoxical role of 3-methyladenine in pyocyanin-induced toxicity in 1321N1 astrocytoma and SH-SY5Y neuroblastoma cells. Int J Toxicol. 2013 May-Jun;32(3):209-18. doi: 10.1177/1091581813482146. PubMed PMID: 23525265.

13: Liu M, He Y, Zhang J. [Effect of autophagy inhibitor 3-methyladenine on proliferation and activation of hepatic stellate cells]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2013 Aug;29(8):809-12. Chinese. PubMed PMID: 23948405.

14: Sheng Y, Sun B, Guo WT, Zhang YH, Liu X, Xing Y, Dong DL. 3-Methyladenine induces cell death and its interaction with chemotherapeutic drugs is independent of autophagy. Biochem Biophys Res Commun. 2013 Mar 1;432(1):5-9. doi: 10.1016/j.bbrc.2013.01.106. PubMed PMID: 23395679.

15: Heckmann BL, Yang X, Zhang X, Liu J. The autophagic inhibitor 3-methyladenine potently stimulates PKA-dependent lipolysis in adipocytes. Br J Pharmacol. 2013 Jan;168(1):163-71. doi: 10.1111/j.1476-5381.2012.02110.x. PubMed PMID: 22817685; PubMed Central PMCID: PMC3570012.

16: Lei FR, Li XQ, Liu H, Zhu RD, Meng QY, Rong JJ. Rapamycin and 3-methyladenine regulate apoptosis and autophagy in bone-derived endothelial progenitor cells. Chin Med J (Engl). 2012 Nov;125(22):4076-82. PubMed PMID: 23158146.

17: Pliyev BK, Menshikov M. Differential effects of the autophagy inhibitors 3-methyladenine and chloroquine on spontaneous and TNF-α-induced neutrophil apoptosis. Apoptosis. 2012 Oct;17(10):1050-65. doi: 10.1007/s10495-012-0738-x. PubMed PMID: 22638980.

18: Liu L, Huang C, He ZG. A TetR family transcriptional factor directly regulates the expression of a 3-methyladenine DNA glycosylase and physically interacts with the enzyme to stimulate its base excision activity in Mycobacterium bovis BCG. J Biol Chem. 2014 Mar 28;289(13):9065-75. doi: 10.1074/jbc.M113.528919. PubMed PMID: 24509852; PubMed Central PMCID: PMC3979383.

19: Lin YC, Kuo HC, Wang JS, Lin WW. Regulation of inflammatory response by 3-methyladenine involves the coordinative actions on Akt and glycogen synthase kinase 3β rather than autophagy. J Immunol. 2012 Oct 15;189(8):4154-64. doi: 10.4049/jimmunol.1102739. PubMed PMID: 22972931.

20: Zhu X, Yan X, Carter LG, Liu H, Graham S, Coote PJ, Naismith J. A model for 3-methyladenine recognition by 3-methyladenine DNA glycosylase I (TAG) from Staphylococcus aureus. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jun 1;68(Pt 6):610-5. doi: 10.1107/S1744309112016363. PubMed PMID: 22684054; PubMed Central PMCID: PMC3370894.