MSC 2032964A
featured

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 523124

CAS#: 1124381-43-6

Description: MSC 2032964A is a potent and selective ASK1 inhibitor (IC50 = 93 nM). It blocks LPS-induced ASK1 and p38 phosphorylation in cultured mouse astrocytes and suppresses neuroinflammation in a mouse EAE model. MSC 2032964A is orally bioavailable and brain penetrant.

Chemical Structure

img
MSC 2032964A
CAS# 1124381-43-6
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 523124
Name: MSC 2032964A
CAS#: 1124381-43-6
Chemical Formula: C16H13F3N6O
Exact Mass: 362.11
Molecular Weight: 362.316
Elemental Analysis: C, 53.04; H, 3.62; F, 15.73; N, 23.20; O, 4.42

Price and Availability

Size Price Availability Quantity
5mg USD 290 2 Weeks
10mg USD 465 2 Weeks
50mg USD 1150 2 Weeks
100mg USD 2200 2 Weeks
Bulk inquiry

Synonym: MSC-2032964A; MSC2032964A; MSC 2032964A.

IUPAC/Chemical Name: N-[5-(Cyclopropylamino)-7-(trifluoromethyl)[1,2,4]triazolo[1,5-a]pyridin-2-yl]-3-pyridinecarboxamide

InChi Key: XUKGFHHTSUKORV-UHFFFAOYSA-N

InChi Code: InChI=1S/C16H13F3N6O/c17-16(18,19)10-6-12(21-11-3-4-11)25-13(7-10)22-15(24-25)23-14(26)9-2-1-5-20-8-9/h1-2,5-8,11,21H,3-4H2,(H,23,24,26)

SMILES Code: O=C(C1=CC=CN=C1)NC2=NN3C(NC4CC4)=CC(C(F)(F)F)=CC3=N2

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, not in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Instruction:
View handling instruction
Biological target: MSC 2032964A is a potent and selective ASK1 inhibitor (IC50 = 93 nM).
In vitro activity: To this aim, this study performed a high-throughput screening with purified ASK1 and have identified one small molecule inhibitor Hit Series. The most potent compound from the Series, MSC1946002A, showed a moderate inhibition of the enzymatic activity of ASK1 (IC50 of 3000 ± 320 nM). The profiling of MSC2032964A revealed an excellent overall in vitro ADME profile. Reference: EMBO Mol Med. 2010 Dec;2(12):504-15. https://pubmed.ncbi.nlm.nih.gov/21064192/
In vivo activity: This study tested MSC2032964A in in vivo inflammation models and found that it blocked LPS-induced ASK1 and p38 phosphorylation in cultured mouse astrocytes (Fig 7A). The disease incidence of EAE was not different between vehicle- and 30 mg/kg MSC2032964A-treated groups, but MSC2032964A induced a significantly attenuated disease course after d18, nearly reproducing the phenotype observed in ASK1−/− mice (Fig 7B). Reference: EMBO Mol Med. 2010 Dec;2(12):504-15. https://pubmed.ncbi.nlm.nih.gov/21064192/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.3 20.00

Preparing Stock Solutions

The following data is based on the product molecular weight 362.32 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: Guo X, Harada C, Namekata K, Matsuzawa A, Camps M, Ji H, Swinnen D, Jorand-Lebrun C, Muzerelle M, Vitte PA, Rückle T, Kimura A, Kohyama K, Matsumoto Y, Ichijo H, Harada T. Regulation of the severity of neuroinflammation and demyelination by TLR-ASK1-p38 pathway. EMBO Mol Med. 2010 Dec;2(12):504-15. doi: 10.1002/emmm.201000103. PMID: 21064192; PMCID: PMC3377347.
In vitro protocol: Guo X, Harada C, Namekata K, Matsuzawa A, Camps M, Ji H, Swinnen D, Jorand-Lebrun C, Muzerelle M, Vitte PA, Rückle T, Kimura A, Kohyama K, Matsumoto Y, Ichijo H, Harada T. Regulation of the severity of neuroinflammation and demyelination by TLR-ASK1-p38 pathway. EMBO Mol Med. 2010 Dec;2(12):504-15. doi: 10.1002/emmm.201000103. PMID: 21064192; PMCID: PMC3377347.
In vivo protocol: Guo X, Harada C, Namekata K, Matsuzawa A, Camps M, Ji H, Swinnen D, Jorand-Lebrun C, Muzerelle M, Vitte PA, Rückle T, Kimura A, Kohyama K, Matsumoto Y, Ichijo H, Harada T. Regulation of the severity of neuroinflammation and demyelination by TLR-ASK1-p38 pathway. EMBO Mol Med. 2010 Dec;2(12):504-15. doi: 10.1002/emmm.201000103. PMID: 21064192; PMCID: PMC3377347.

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Kawarazaki Y, Ichijo H, Naguro I. Apoptosis signal-regulating kinase 1 as a therapeutic target. Expert Opin Ther Targets. 2014 Jun;18(6):651-64. doi: 10.1517/14728222.2014.896903. Epub 2014 Mar 24. Review. PubMed PMID: 24660755.