Salinomycin
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MedKoo CAT#: 406545

CAS#: 53003-10-4

Description: Salinomycin is an antibacterial and coccidiostat ionophore agent. Salinomycin suppresses T24 cells by regulating KDM1A and the unfolded protein response pathway. Salinomycin alleviates osteoarthritis progression via inhibiting Wnt/β-catenin signaling. Vitamin D3 and Salinomycin synergy in MCF-7 cells cause cell death via endoplasmic reticulum stress in monolayer and 3D cell culture. Salinomycin induces cell cycle arrest and apoptosis and modulates hepatic cytochrome P450 mRNA expression in HepG2/C3a cells. Salinomycin inhibits proliferation and induces apoptosis of human hepatocellular carcinoma cells in vitro and in vivo,

Chemical Structure

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Salinomycin
CAS# 53003-10-4
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 406545
Name: Salinomycin
CAS#: 53003-10-4
Chemical Formula: C42H70O11
Exact Mass: 750.49
Molecular Weight: 751.011
Elemental Analysis: C, 67.17; H, 9.40; O, 23.43

Price and Availability

Size Price Availability Quantity
1mg USD 90 Ready to Ship
5mg USD 350 Ready to Ship
10mg USD 550 Ready to ship
25mg USD 750 Ready to Ship
50mg USD 1250 Ready to Ship
100mg USD 2050 Ready to Ship
200mg USD 3650 Ready to ship
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Synonym: AHR 3096; AHR3096; AHR-3096; HSDB 7032; HSDB7032; HSDB-7032; K 364; Salinomycin; BioCox; Sacox; Salocin; Coxistac.

IUPAC/Chemical Name: (R)-2-((2R,5S,6R)-6-((2S,3S,4S,6R)-6-((2S,5S,7R,9S,10S,12R,15R)-2-((2R,5R,6S)-5-ethyl-5-hydroxy-6-methyltetrahydro-2H-pyran-2-yl)-15-hydroxy-2,10,12-trimethyl-1,6,8-trioxadispiro[4.1.57.35]pentadec-13-en-9-yl)-3-hydroxy-4-methyl-5-oxooctan-2-yl)-5-methyltetrahydro-2H-pyran-2-yl)butanoic acid

InChi Key: KQXDHUJYNAXLNZ-XQSDOZFQSA-N

InChi Code: InChI=1S/C42H70O11/c1-11-29(38(46)47)31-15-14-23(4)36(50-31)27(8)34(44)26(7)35(45)30(12-2)37-24(5)22-25(6)41(51-37)19-16-32(43)42(53-41)21-20-39(10,52-42)33-17-18-40(48,13-3)28(9)49-33/h16,19,23-34,36-37,43-44,48H,11-15,17-18,20-22H2,1-10H3,(H,46,47)/t23-,24-,25+,26-,27-,28-,29+,30-,31+,32+,33+,34+,36+,37-,39-,40+,41-,42-/m0/s1

SMILES Code: CC[C@@H](C(O)=O)[C@H]1CC[C@@H]([C@H]([C@H]([C@@H]([C@@H](C([C@@H]([C@H]2O[C@]3([C@@H](C[C@@H]2C)C)O[C@]4([C@@H](C=C3)O)CC[C@](O4)([C@H]5CC[C@](CC)([C@@H](O5)C)O)C)CC)=O)C)O)C)O1)C

Appearance: brown solid powder

Purity: 98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, not in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:   The ability of salinomycin to kill both CSCs and therapy-resistant cancer cells may define the compound as a novel and an effective anticancer drug. It has been also shown that Salinomycin and its derivatives exhibit potent antiproliferative activity against the drug-resistant cancer cell lines.      

Biological target: Salinomycin (Procoxacin), a polyether potassium ionophore antibiotic, selectively inhibits the growth of gram-positive bacteria.
In vitro activity: The metastasis and invasion abilities of serum bladder cancer cell line T24 after salinomycin treatment in the experiment group were significantly reduced when compared with those in the control group, and the tumor metastasis lesions were decreased from an average of 1.59 to 0.6 (P < 0.05). Salinomycin can suppress the metastasis and invasion of bladder cancer cells, of which the mechanism is probably associated with the inhibition of EMT of tumor cells. Reference: Asian Pac J Trop Med. 2015 Jul;8(7):578-82. https://pubmed.ncbi.nlm.nih.gov/26276292/
In vivo activity: The anti-tumor effect of Sal (Salinomycin) was further verified in vivo using the hepatoma orthotopic tumor model and the data obtained showed that the size of liver tumors in Sal-treated groups decreased compared to controls. Immunohistochemistry and TUNEL staining also demonstrated that Sal inhibits proliferation and induces apoptosis in vivo. Reference: PLoS One. 2012;7(12):e50638. https://pubmed.ncbi.nlm.nih.gov/23284640/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 20.0 26.63
DMF:PBS (pH 7.2) (1:4) 0.2 0.27
DMSO 47.2 62.89
Ethanol 12.5 16.64

Preparing Stock Solutions

The following data is based on the product molecular weight 751.01 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Qu H, Ma B, Yuan HF, Wang ZY, Guo SJ, Zhang J. Effect of salinomycin on metastasis and invasion of bladder cancer cell line T24. Asian Pac J Trop Med. 2015 Jul;8(7):578-82. doi: 10.1016/j.apjtm.2015.06.004. Epub 2015 Jul 9. PMID: 26276292. 2. Verdoodt B, Vogt M, Schmitz I, Liffers ST, Tannapfel A, Mirmohammadsadegh A. Salinomycin induces autophagy in colon and breast cancer cells with concomitant generation of reactive oxygen species. PLoS One. 2012;7(9):e44132. doi: 10.1371/journal.pone.0044132. Epub 2012 Sep 19. PMID: 23028492; PMCID: PMC3446972. 3. Klose J, Trefz S, Wagner T, Steffen L, Preißendörfer Charrier A, Radhakrishnan P, Volz C, Schmidt T, Ulrich A, Dieter SM, Ball C, Glimm H, Schneider M. Salinomycin: Anti-tumor activity in a pre-clinical colorectal cancer model. PLoS One. 2019 Feb 14;14(2):e0211916. doi: 10.1371/journal.pone.0211916. PMID: 30763370; PMCID: PMC6375586. 4. Wang F, He L, Dai WQ, Xu YP, Wu D, Lin CL, Wu SM, Cheng P, Zhang Y, Shen M, Wang CF, Lu J, Zhou YQ, Xu XF, Xu L, Guo CY. Salinomycin inhibits proliferation and induces apoptosis of human hepatocellular carcinoma cells in vitro and in vivo. PLoS One. 2012;7(12):e50638. doi: 10.1371/journal.pone.0050638. Epub 2012 Dec 20. PMID: 23284640; PMCID: PMC3527475.
In vitro protocol: 1. Qu H, Ma B, Yuan HF, Wang ZY, Guo SJ, Zhang J. Effect of salinomycin on metastasis and invasion of bladder cancer cell line T24. Asian Pac J Trop Med. 2015 Jul;8(7):578-82. doi: 10.1016/j.apjtm.2015.06.004. Epub 2015 Jul 9. PMID: 26276292. 2. Verdoodt B, Vogt M, Schmitz I, Liffers ST, Tannapfel A, Mirmohammadsadegh A. Salinomycin induces autophagy in colon and breast cancer cells with concomitant generation of reactive oxygen species. PLoS One. 2012;7(9):e44132. doi: 10.1371/journal.pone.0044132. Epub 2012 Sep 19. PMID: 23028492; PMCID: PMC3446972.
In vivo protocol: 1. Klose J, Trefz S, Wagner T, Steffen L, Preißendörfer Charrier A, Radhakrishnan P, Volz C, Schmidt T, Ulrich A, Dieter SM, Ball C, Glimm H, Schneider M. Salinomycin: Anti-tumor activity in a pre-clinical colorectal cancer model. PLoS One. 2019 Feb 14;14(2):e0211916. doi: 10.1371/journal.pone.0211916. PMID: 30763370; PMCID: PMC6375586. 2. Wang F, He L, Dai WQ, Xu YP, Wu D, Lin CL, Wu SM, Cheng P, Zhang Y, Shen M, Wang CF, Lu J, Zhou YQ, Xu XF, Xu L, Guo CY. Salinomycin inhibits proliferation and induces apoptosis of human hepatocellular carcinoma cells in vitro and in vivo. PLoS One. 2012;7(12):e50638. doi: 10.1371/journal.pone.0050638. Epub 2012 Dec 20. PMID: 23284640; PMCID: PMC3527475.

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1: Lee J, Roh JL. Promotion of ferroptosis in head and neck cancer with divalent metal transporter 1 inhibition or salinomycin. Hum Cell. 2023 Mar 8. doi: 10.1007/s13577-023-00890-x. Epub ahead of print. PMID: 36890422.


2: Anees M, Mehrotra N, Tiwari S, Kumar D, Kharbanda S, Singh H. Polylactic acid based biodegradable hybrid block copolymeric nanoparticle mediated co-delivery of salinomycin and doxorubicin for cancer therapy. Int J Pharm. 2023 Feb 24;635:122779. doi: 10.1016/j.ijpharm.2023.122779. Epub ahead of print. PMID: 36842520.


3: Chen M, Yang Y, Ying Y, Huang J, Sun M, Hong M, Wang H, Xie S, Chen D. ABC Transporters and CYP3A4 Mediate Drug Interactions between Enrofloxacin and Salinomycin Leading to Increased Risk of Drug Residues and Resistance. Antibiotics (Basel). 2023 Feb 17;12(2):403. doi: 10.3390/antibiotics12020403. PMID: 36830313; PMCID: PMC9952136.


4: Petrova E, Gluhcheva Y, Pavlova E, Vladov I, Dorkov P, Schaier M, Pashkunova- Martic I, Helbich TH, Keppler B, Ivanova J. Effects of Salinomycin and Deferiprone on Lead-Induced Changes in the Mouse Brain. Int J Mol Sci. 2023 Feb 2;24(3):2871. doi: 10.3390/ijms24032871. PMID: 36769197; PMCID: PMC9918121.


5: Martyniuk V, Khoma V, Matskiv T, Yunko K, Gnatyshyna L, Stoliar O, Faggio C. Combined effect of microplastic, salinomycin and heating on Unio tumidus. Environ Toxicol Pharmacol. 2023 Mar;98:104068. doi: 10.1016/j.etap.2023.104068. Epub 2023 Jan 19. PMID: 36680920.


6: Xi HM, Lu H, Weng XQ, Sheng Y, Wu J, Li L, Cai X. Combined Application of Salinomycin and ATRA Induces Apoptosis and Differentiation of Acute Myeloid Leukemia Cells by Inhibiting WNT/β-catenin Pathway. Anticancer Agents Med Chem. 2023 Jan 10. doi: 10.2174/1871520623666230110121629. Epub ahead of print. PMID: 36627782.


7: Tefas LR, Toma I, Sesarman A, Banciu M, Jurj A, Berindan-Neagoe I, Rus L, Stiufiuc R, Tomuta I. Co-delivery of gemcitabine and salinomycin in PEGylated liposomes for enhanced anticancer efficacy against colorectal cancer. J Liposome Res. 2022 Dec 15:1-17. doi: 10.1080/08982104.2022.2153139. Epub ahead of print. PMID: 36472146.


8: Pashkunova-Martic I, Kukeva R, Stoyanova R, Pantcheva I, Dorkov P, Friske J, Hejl M, Jakupec M, Hohagen M, Legin A, Lubitz W, Keppler BK, Helbich TH, Ivanova J. Novel Salinomycin-Based Paramagnetic Complexes-First Evaluation of Their Potential Theranostic Properties. Pharmaceutics. 2022 Oct 28;14(11):2319. doi: 10.3390/pharmaceutics14112319. PMID: 36365139; PMCID: PMC9692412.


9: Ghonaim AH, Hopo MG, Ismail AK, AboElnaga TR, Elgawish RA, Abdou RH, Elhady KA. Hepatoprotective and renoprotective effects of silymarin against salinomycin-induced toxicity in adult rabbits. Vet World. 2022 Sep;15(9):2244-2252. doi: 10.14202/vetworld.2022.2244-2252. Epub 2022 Sep 19. PMID: 36341068; PMCID: PMC9631362.


10: Orso C, Cony BL, Silva JP, Furtado JCV, Mann MB, Frazzon J, Frazzon APG, Andretta I, Ribeiro AML. Effect of live Eimeria vaccination or salinomycin on growth and immune status in broiler chickens receiving in-feed inclusion of gelatin and vitamin E. Poult Sci. 2022 Dec;101(12):102206. doi: 10.1016/j.psj.2022.102206. Epub 2022 Oct 1. PMID: 36334427; PMCID: PMC9627594.


11: Yuan H, Li Y, Zou Y, Cai C, Shi X, Su Y. Salinomycin suppresses T24 cells by regulating KDM1A and the unfolded protein response pathway. Cytotechnology. 2022 Oct;74(5):579-590. doi: 10.1007/s10616-022-00546-y. Epub 2022 Sep 6. PMID: 36238269; PMCID: PMC9525558.


12: Takeda S, Hirao-Suzuki M, Shindo M, Aramaki H. (-)-Xanthatin as a Killer of Human Breast Cancer MCF-7 Mammosphere Cells: A Comparative Study with Salinomycin. Curr Issues Mol Biol. 2022 Aug 25;44(9):3849-3858. doi: 10.3390/cimb44090264. PMID: 36135176; PMCID: PMC9497939.


13: Chang Y, Geng Q, Bao Q, Hu P. Retraction Note: Salinomycin enhances radiotherapy sensitivity and reduces expressions of BIRC5 and NEIL2 in nasopharyngeal carcinoma. Eur Rev Med Pharmacol Sci. 2022 Sep;26(17):6012. doi: 10.26355/eurrev_202209_29611. PMID: 36111899.


14: Zou M, Yin X, Zhou X, Niu X, Wang Y, Su M. Salinomycin-Loaded High-Density Lipoprotein Exerts Promising Anti-Ovarian Cancer Effects by Inhibiting Epithelial-Mesenchymal Transition. Int J Nanomedicine. 2022 Sep 8;17:4059-4071. doi: 10.2147/IJN.S380598. PMID: 36105618; PMCID: PMC9467852.


15: Wei J, Chen B, Dong J, Wang X, Li Y, Liu Y, Guan W. Salinomycin biosynthesis reversely regulates the β-oxidation pathway in Streptomyces albus by carrying a 3-hydroxyacyl-CoA dehydrogenase gene in its biosynthetic gene cluster. Microb Biotechnol. 2022 Dec;15(12):2890-2904. doi: 10.1111/1751-7915.14145. Epub 2022 Sep 13. PMID: 36099515; PMCID: PMC9733648.


16: Chen J, Liu J, Chen S, Lai R, Zheng C, Lu J, Jiang X, He F, Yang C, Li K, Xie K, Tang Y, Wang L. Salinomycin alleviates osteoarthritis progression via inhibiting Wnt/β-catenin signaling. Int Immunopharmacol. 2022 Nov;112:109225. doi: 10.1016/j.intimp.2022.109225. Epub 2022 Sep 9. PMID: 36095950.


17: Qin H, Guo Y. Targeting Telomerase Enhances Cytotoxicity of Salinomycin in Cancer Cells. ACS Omega. 2022 Aug 17;7(34):30565-30570. doi: 10.1021/acsomega.2c04082. PMID: 36061682; PMCID: PMC9435028.


18: Cheng X, Zheng H, Wang C, Wang X, Fei C, Zhou W, Zhang K. Effects of salinomycin and ethanamizuril on the three microbial communities in vivo and in vitro. Front Microbiol. 2022 Aug 12;13:941259. doi: 10.3389/fmicb.2022.941259. PMID: 36033856; PMCID: PMC9413843.


19: Marques LA, Semprebon SC, Biazi BI, Felicidade I, Zanetti TA, Baranoski A, Concato VM, Pavanelli WR, Mantovani MS. Vitamin D3 and Salinomycin synergy in MCF-7 cells cause cell death via endoplasmic reticulum stress in monolayer and 3D cell culture. Toxicol Appl Pharmacol. 2022 Oct 1;452:116178. doi: 10.1016/j.taap.2022.116178. Epub 2022 Jul 29. PMID: 35914560.


20: Savaee M, Bakhshi A, Yaghoubi F, Pourrajab F, Goodarzvand Chegini K. Evaluating the Effects of Separate and Concomitant Use of MK-2206 and Salinomycin on Prostate Cancer Cell Line. Rep Biochem Mol Biol. 2022 Apr;11(1):157-165. doi: 10.52547/rbmb.11.1.157. PMID: 35765523; PMCID: PMC9208569.