J27

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MedKoo CAT#: 124749

CAS#: N/A

Description: J27 is a NF-κB/MAPK inhibitor. J27decreased the release of TNF-αandIL-6 in mouse and human cells J774A.1 and THP-1 (IL-6 IC50= 0.22μM )through the NF-κB/MAPK pathway. J27 demonstrated remarkable protection against ALI and sepsis in vivo and exhibited good safety in subacute toxicity experiments. Pharmacokinetic study indicated that J27 had good bioavailability (30.74%).To oursurprise,J27 could targe tJNK2 with a totally new molecular skeleton compared with the only few JNK2 inhibitors reported. Moreover, there is no reportthat JNK2 inhibitors could apply for ALI and sepsis.Therefore, this work provides a new lead structure for the study of JNK2inhibitorsanda new target of JNK2to treat ALI and sepsis.

Chemical Structure

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J27
CAS# N/A
Instruction

Theoretical Analysis

MedKoo Cat#: 124749
Name: J27
CAS#: N/A
Chemical Formula: C30H26N4O5
Exact Mass: 522.19
Molecular Weight: 522.560
Elemental Analysis: C, 68.95; H, 5.02; N, 10.72; O, 15.31

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: J27; J 27; J-27

IUPAC/Chemical Name: N-(4-((6,7-dimethoxyquinolin-4-yl)oxy)phenyl)-4-methyl-6-oxo-1-(p-tolyl)-1,6-dihydropyridazine-3-carboxamide

InChi Key: RHGFVWDZWMEFNR-UHFFFAOYSA-N

InChi Code: InChI=1S/C30H26N4O5/c1-18-5-9-21(10-6-18)34-28(35)15-19(2)29(33-34)30(36)32-20-7-11-22(12-8-20)39-25-13-14-31-24-17-27(38-4)26(37-3)16-23(24)25/h5-17H,1-4H3,(H,32,36)

SMILES Code: CC1=CC(N(C2=CC=C(C)C=C2)N=C1C(NC3=CC=C(OC4=CC=NC5=CC(OC)=C(OC)C=C54)C=C3)=O)=O

Appearance: To be determined

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 522.56 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Liao J, Yang J, Li X, Hu C, Zhu W, Zhou Y, Zou Y, Guo M, Chen Z, Li X, Dai J,
Xu Y, Zheng Z, Chen P, Cho WJ, Liang G, Tang Q. Discovery of the Diphenyl
6-Oxo-1,6-dihydropyridazine-3-carboxylate/carboxamide Analogue J27 for the
Treatment of Acute Lung Injury and Sepsis by Targeting JNK2 and Inhibiting the
JNK2-NF-κB/MAPK Pathway. J Med Chem. 2023 Aug 29. doi:
10.1021/acs.jmedchem.3c00832. Epub ahead of print. PMID: 37643372.

2: Cuevas-González PF, Aguilar-Toalá JE, García HS, González-Córdova AF,
Vallejo-Cordoba B, Hernández-Mendoza A. Protective Effect of the Intracellular
Content from Potential Probiotic Bacteria against Oxidative Damage Induced by
Acrylamide in Human Erythrocytes. Probiotics Antimicrob Proteins. 2020
Dec;12(4):1459-1470. doi: 10.1007/s12602-020-09636-9. PMID: 31970648.

3: Bhatt JD, Chudasama CJ, Patel KD. Diarylpyrazole Ligated Dihydropyrimidine
Hybrids as Potent Non-Classical Antifolates and Their Efficacy Against
Plasmodium falciparum. Arch Pharm (Weinheim). 2017 Sep;350(9). doi:
10.1002/ardp.201700088. Epub 2017 Aug 10. PMID: 28796406.

4: Bhatt JD, Chudasama CJ, Patel KD. Microwave Assisted Synthesis of Pyrimidines
in Ionic Liquid and Their Potency as Non-Classical Malarial Antifolates. Arch
Pharm (Weinheim). 2016 Oct;349(10):791-800. doi: 10.1002/ardp.201600148. Epub
2016 Aug 16. PMID: 27528517.