GP-AMC HBr
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MedKoo CAT#: 465000

CAS#: 115035-46-6

Description: GP-AMC is a fluorogenic substrate for dipeptidyl peptidase 4 (DPP-4). Upon enzymatic cleavage by DPP-4, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify DPP-4 activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

Chemical Structure

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GP-AMC HBr
CAS# 115035-46-6
Instruction

Theoretical Analysis

MedKoo Cat#: 465000
Name: GP-AMC HBr
CAS#: 115035-46-6
Chemical Formula: C17H20BrN3O4
Exact Mass: 409.06
Molecular Weight: 410.268
Elemental Analysis: C, 49.77; H, 4.91; Br, 19.48; N, 10.24; O, 15.60

Price and Availability

Size Price Availability Quantity
5mg USD 260 2 Weeks
10mg USD 430 2 Weeks
25mg USD 790 2 Weeks
50mg USD 1250 2 Weeks
Bulk inquiry

Synonym: GP-AMC HBr; GP AMC HBr; GPAMC HBr; GP-AMC hydrobromide; GPAMC hydrobromide; GP AMC hydrobromide; Gly-Pro-AMC; Gly-Pro-7-amido-4-methylcoumarin;

IUPAC/Chemical Name: (S)-1-glycyl-N-(4-methyl-2-oxo-2H-chromen-7-yl)pyrrolidine-2-carboxamide hydrobromide

InChi Key: PASXTQMFJRHMLJ-ZOWNYOTGSA-N

InChi Code: InChI=1S/C17H19N3O4.BrH/c1-10-7-16(22)24-14-8-11(4-5-12(10)14)19-17(23)13-3-2-6-20(13)15(21)9-18;/h4-5,7-8,13H,2-3,6,9,18H2,1H3,(H,19,23);1H/t13-;/m0./s1

SMILES Code: CC(C1=CC=C(C=C1O2)NC([C@H]3N(CCC3)C(CN)=O)=O)=CC2=O.Br

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 10.0 24.37
DMSO 1.0 2.44
PBS (pH 7.2) 10.0 24.37

Preparing Stock Solutions

The following data is based on the product molecular weight 410.27 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Zhang J, Qian XK, Song PF, Li XD, Wang AQ, Huo H, Yao JC, Zhang GM, Zou LW. A high-throughput screening assay for dipeptidyl peptidase-IV inhibitors using human plasma. Anal Methods. 2021 Jun 24;13(24):2671-2678. doi: 10.1039/d1ay00415h. PMID: 34036983.