Ro 32-0432 HCl
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MedKoo CAT#: 462220

CAS#: 1781828-85-0

Description: Ro 32-0432 HCl is a potent, selective, ATP-competitive and orally active PKC inhibitor.

Chemical Structure

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Ro 32-0432 HCl
CAS# 1781828-85-0
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 462220
Name: Ro 32-0432 HCl
CAS#: 1781828-85-0
Chemical Formula: C28H29ClN4O2
Exact Mass: 0.00
Molecular Weight: 489.020
Elemental Analysis: C, 68.77; H, 5.98; Cl, 7.25; N, 11.46; O, 6.54

Price and Availability

Size Price Availability Quantity
1mg USD 395 2 Weeks
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Synonym: Ro 32-0432 hydrochloride, Ro 32-0432 HCl, Ro 32 0432 HCl, Ro 320432 HCl, Ro32-0432 HCl

IUPAC/Chemical Name: (S)-3-(8-((dimethylamino)methyl)-6,7,8,9-tetrahydropyrido[1,2-a]indol-10-yl)-4-(1-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione hydrochloride

InChi Key: HSPRASOZRZDELU-LMOVPXPDSA-N

InChi Code: InChI=1S/C28H28N4O2.ClH/c1-30(2)15-17-12-13-32-22-11-7-5-9-19(22)24(23(32)14-17)26-25(27(33)29-28(26)34)20-16-31(3)21-10-6-4-8-18(20)21;/h4-11,16-17H,12-15H2,1-3H3,(H,29,33,34);1H/t17-;/m0./s1

SMILES Code: O=C1NC(C(C2=C(C[C@@H](CN(C)C)CC3)N3C4=C2C=CC=C4)=C1C5=CN(C)C6=C5C=CC=C6)=O.[H]Cl

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Product Data
Instruction:
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Biological target: Ro 32-0432 HCl is a potent, selective, ATP-competitive and orally active PKC inhibitor. The IC50 values of Ro 32-0432 HCl for PKCα, PKCβI, PKCβII, PKCγ and PKCε are 9.3 nM, 28 nM, 30 nM, 36.5 nM and 108.3 nM, respectively. Ro 32-0432 HCl prevents T-cell activation.
In vitro activity: NECA-mediated IL-6 release was inhibited by 1-[6-((17beta-3-methoxyestra-1,3,5(10)-tiene-17-yl)amino)hexyl]-1H-pyrrole-2,5-dione, wortmannin, and Ro-32-0432. This study investigated the action of adenosine A2 receptor agonists on IL-6 and VEGF secretion in two murine FS cell lines (TtT/GF and Tpit/F1), and demonstrated a rank order of potency. Reference: Br J Pharmacol. 2003 Oct;140(4):764-72. https://pubmed.ncbi.nlm.nih.gov/14504137/
In vivo activity: In vivo injection with chelerythrine chloride or Ro-32-0432 into the ventral tegmental area (VTA) significantly suppressed the place preference and increased levels of dopamine in the nucleus accumbens induced by intra-VTA injection of orexins. Reference: Eur J Neurosci. 2007 Mar;25(5):1537-45. https://pubmed.ncbi.nlm.nih.gov/17425580/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 4.9 10.00

Preparing Stock Solutions

The following data is based on the product molecular weight 489.02 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Rees DA, Lewis BM, Lewis MD, Francis K, Scanlon MF, Ham J. Adenosine-induced IL-6 expression in pituitary folliculostellate cells is mediated via A2b adenosine receptors coupled to PKC and p38 MAPK. Br J Pharmacol. 2003 Oct;140(4):764-72. doi: 10.1038/sj.bjp.0705488. Epub 2003 Sep 22. PMID: 14504137; PMCID: PMC1574075. 2. Narita M, Nagumo Y, Miyatake M, Ikegami D, Kurahashi K, Suzuki T. Implication of protein kinase C in the orexin-induced elevation of extracellular dopamine levels and its rewarding effect. Eur J Neurosci. 2007 Mar;25(5):1537-45. doi: 10.1111/j.1460-9568.2007.05403.x. PMID: 17425580.
In vitro protocol: 1. Rees DA, Lewis BM, Lewis MD, Francis K, Scanlon MF, Ham J. Adenosine-induced IL-6 expression in pituitary folliculostellate cells is mediated via A2b adenosine receptors coupled to PKC and p38 MAPK. Br J Pharmacol. 2003 Oct;140(4):764-72. doi: 10.1038/sj.bjp.0705488. Epub 2003 Sep 22. PMID: 14504137; PMCID: PMC1574075.
In vivo protocol: 1. Narita M, Nagumo Y, Miyatake M, Ikegami D, Kurahashi K, Suzuki T. Implication of protein kinase C in the orexin-induced elevation of extracellular dopamine levels and its rewarding effect. Eur J Neurosci. 2007 Mar;25(5):1537-45. doi: 10.1111/j.1460-9568.2007.05403.x. PMID: 17425580.

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1: Birchall AM, Bishop J, Bradshaw D, et al. Ro 32-0432, a selective and orally active inhibitor of protein kinase C prevents T-cell activation. J Pharmacol Exp Ther. 1994;268(2):922-929.

2: Singh TG, Rehni AK, Arora S. Ro 32-0432 attenuates mecamylamine-precipitated nicotine withdrawal syndrome in mice. Naunyn Schmiedebergs Arch Pharmacol. 2013;386(3):197-204. doi:10.1007/s00210-012-0825-0