Lysine chloromethyl ketone

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 530753

CAS#: 26995-77-7

Description: Lysine chloromethyl ketone is an affinity label for peptides.

Chemical Structure

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Lysine chloromethyl ketone
CAS# 26995-77-7
Instruction

Theoretical Analysis

MedKoo Cat#: 530753
Name: Lysine chloromethyl ketone
CAS#: 26995-77-7
Chemical Formula: C7H15ClN2O
Exact Mass: 178.09
Molecular Weight: 178.660
Elemental Analysis: C, 47.06; H, 8.46; Cl, 19.84; N, 15.68; O, 8.95

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

Request quote for custom synthesis

Synonym: Lysine chloromethyl ketone

IUPAC/Chemical Name: 2-Heptanone, 3,7-diamino-1-chloro-, (S)-

InChi Key: PDBIHKPGRQQIEC-LURJTMIESA-N

InChi Code: InChI=1S/C7H15ClN2O/c8-5-7(11)6(10)3-1-2-4-9/h6H,1-5,9-10H2/t6-/m0/s1

SMILES Code: O=C([C@@H](N)CCCCN)CCl

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 178.66 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Jadhav AR, War AR, Nikam AN, Adhav AS, Gupta VS, Sharma HC, Giri AP, Tamhane VA. Capsicum annuum proteinase inhibitor ingestion negatively impacts the growth of sorghum pest Chilo partellus and promotes differential protease expression. Biochem Biophys Rep. 2016 Oct 6;8:302-309. doi: 10.1016/j.bbrep.2016.09.016. eCollection 2016 Dec. PubMed PMID: 28955969; PubMed Central PMCID: PMC5614469.

2: Hernandez-Cortes P, Rivera-Pérez C, García-Carreño F, Martínez-Alarcón D. Proteinases during Early Development of the Pacific Whiteleg Shrimp Penaeus vannamei. Biol Bull. 2017 Feb;232(1):2-11. doi: 10.1086/691381. Epub 2017 Apr 3. PubMed PMID: 28445094.

3: Wan HJ, Lyu W, Yu L, Zhou ZY, Hu YJ, Hu J. [Inhibition of G9a attenuates cell proliferation via the mitochondrial apoptosis pathway in lung adenocarcinoma]. Zhonghua Zhong Liu Za Zhi. 2017 Jan 23;39(1):13-17. doi: 10.3760/cma.j.issn.0253-3766.2017.01.003. Chinese. PubMed PMID: 28104027.

4: França RCDP, Assis CRD, Santos JF, Torquato RJS, Tanaka AS, Hirata IY, Assis DM, Juliano MA, Cavalli RO, Carvalho LB Jr, Bezerra RS. Bovine pancreatic trypsin inhibitor immobilized onto sepharose as a new strategy to purify a thermostable alkaline peptidase from cobia (Rachycentron canadum) processing waste. J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Oct 15;1033-1034:210-217. doi: 10.1016/j.jchromb.2016.08.028. Epub 2016 Aug 18. PubMed PMID: 27567377.

5: Wei J, Liang G, Wang B, Zhong F, Chen L, Khaing MM, Zhang J, Guo Y, Wu K, Tabashnik BE. Activation of Bt Protoxin Cry1Ac in Resistant and Susceptible Cotton Bollworm. PLoS One. 2016 Jun 3;11(6):e0156560. doi: 10.1371/journal.pone.0156560. eCollection 2016. PubMed PMID: 27257885; PubMed Central PMCID: PMC4892611.

6: Sun Z, Liu P, Cheng G, Zhang B, Dong W, Su X, Huang Y, Cui Z, Kong Y. A fibrinolytic protease AfeE from Streptomyces sp. CC5, with potent thrombolytic activity in a mouse model. Int J Biol Macromol. 2016 Apr;85:346-54. doi: 10.1016/j.ijbiomac.2015.12.059. Epub 2015 Dec 22. PubMed PMID: 26721382.

7: Berthold T, Schubert N, Muschter S, Rohr M, Wesche J, Reil A, Bux J, Bakchoul T, Greinacher A. HNA antibody-mediated neutrophil aggregation is dependent on serine protease activity. Vox Sang. 2015 Nov;109(4):366-74. doi: 10.1111/vox.12292. Epub 2015 Jun 17. PubMed PMID: 26084778.

8: Chakraborti T, Das P, Choudhury R, De T. Effect of different serine protease inhibitors in validating the 115 kDa Leishmania donovani secretory serine protease as chemotherapeutic target. Indian J Biochem Biophys. 2015 Feb;52(1):14-22. PubMed PMID: 26040107.

9: Voigt J, Woestemeyer J. Protease Inhibitors Cause Necrotic Cell Death in Chlamydomonas reinhardtii by Inducing the Generation of Reactive Oxygen Species. J Eukaryot Microbiol. 2015 Nov-Dec;62(6):711-21. doi: 10.1111/jeu.12224. Epub 2015 May 27. PubMed PMID: 25939325.

10: Beek J, Maes D, Nauwynck H, Piepers S, Van Soom A. A critical assessment of the effect of serine protease inhibitors on porcine fertilization and quality parameters of porcine spermatozoa in vitro. Reprod Biol. 2015 Mar;15(1):9-19. doi: 10.1016/j.repbio.2014.12.002. Epub 2015 Jan 7. PubMed PMID: 25726372.

11: Wielkopolan B, Walczak F, Podleśny A, Nawrot R, Obrępalska-Stęplowska A. Identification and partial characterization of proteases in larval preparations of the cereal leaf beetle (Oulema melanopus, Chrysomelidae, Coleoptera). Arch Insect Biochem Physiol. 2015 Mar;88(3):192-202. doi: 10.1002/arch.21223. Epub 2015 Jan 12. PubMed PMID: 25580929.

12: Khangembam BK, Chakrabarti R. Trypsin from the digestive system of carp Cirrhinus mrigala: purification, characterization and its potential application. Food Chem. 2015 May 15;175:386-94. doi: 10.1016/j.foodchem.2014.11.140. Epub 2014 Nov 28. PubMed PMID: 25577096.

13: Shadrin N, Shapira MG, Khalfin B, Uppalapati L, Parola AH, Nathan I. Serine protease inhibitors interact with IFN-γ through up-regulation of FasR; a novel therapeutic strategy against cancer. Exp Cell Res. 2015 Jan 15;330(2):233-9. doi: 10.1016/j.yexcr.2014.11.005. Epub 2014 Nov 18. PubMed PMID: 25449698.

14: Reuther C, Ganjam GK, Dolga AM, Culmsee C. The serine protease inhibitor TLCK attenuates intrinsic death pathways in neurons upstream of mitochondrial demise. Apoptosis. 2014 Nov;19(11):1545-58. doi: 10.1007/s10495-014-1027-7. PubMed PMID: 25146045.

15: Lee EH, Park JE, Park JW, Lee JS. Purification and biochemical characterization of a fibrin(ogen)olytic metalloprotease from Macrovipera mauritanica snake venom which induces vascular permeability. Int J Mol Med. 2014 Oct;34(4):1180-90. doi: 10.3892/ijmm.2014.1864. Epub 2014 Jul 24. PubMed PMID: 25069477.

16: Qu L, Vongpunsawad S, Atmar RL, Prasad BV, Estes MK. Development of a Gaussia luciferase-based human norovirus protease reporter system: cell type-specific profile of Norwalk virus protease precursors and evaluation of inhibitors. J Virol. 2014 Sep;88(18):10312-26. doi: 10.1128/JVI.01111-14. Epub 2014 Jul 9. PubMed PMID: 25008934; PubMed Central PMCID: PMC4178847.

17: Kotb E. Purification and partial characterization of a chymotrypsin-like serine fibrinolytic enzyme from Bacillus amyloliquefaciens FCF-11 using corn husk as a novel substrate. World J Microbiol Biotechnol. 2014 Jul;30(7):2071-80. doi: 10.1007/s11274-014-1632-1. Epub 2014 Mar 8. PubMed PMID: 24609498.

18: Zhang JY, Fu Y, Song XC, Liang M. [Expression of integrin α5 and β1 in osteoblast in the process of gingipains-induced apoptosis]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2013 Nov;48(11):653-9. Chinese. PubMed PMID: 24513069.

19: Wang ZH, Zhu W, Tao JP, Zhang QY, Wei M. Stimulated mast cells promote maturation of myocardial microvascular endothelial cell neovessels by modulating the angiopoietin-Tie-2 signaling pathway. Braz J Med Biol Res. 2013 Nov;46(11):920-928. Epub 2013 Oct 22. PubMed PMID: 24270910; PubMed Central PMCID: PMC3854333.

20: Suthindhiran K, Jayasri MA, Dipali D, Prasar A. Screening and characterization of protease producing actinomycetes from marine saltern. J Basic Microbiol. 2014 Oct;54(10):1098-109. doi: 10.1002/jobm.201300563. Epub 2013 Oct 18. PubMed PMID: 24136565.