H8 dihydrochloride
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 540190

CAS#: 113276-94-1

Description: H8 dihydrochloride is a PKA inhibitor that modulates Ca 2+ signaling.


Price and Availability

Size
Price

5mg
USD 175
Size
Price

10mg
USD 290
Size
Price

25mg
USD 540

H8 dihydrochloride, purity > 98%, is in stock. Current shipping out time is about 2 weeks after order is received. CoA, QC data and MSDS documents are available in one week after order is received.


Chemical Structure

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Theoretical Analysis

MedKoo Cat#: 540190
Name: H8 dihydrochloride
CAS#: 113276-94-1
Chemical Formula: C12H17Cl2N3O2S
Exact Mass: 337.0419
Molecular Weight: 338.24
Elemental Analysis: C, 42.61; H, 5.07; Cl, 20.96; N, 12.42; O, 9.46; S, 9.48


Synonym: H8; H8 dihydrochloride.

IUPAC/Chemical Name: N-(2-(methylamino)ethyl)isoquinoline-5-sulfonamide dihydrochloride

InChi Key: RJJLZYZEVNCZIW-UHFFFAOYSA-N

InChi Code: InChI=1S/C12H15N3O2S.2ClH/c1-13-7-8-15-18(16,17)12-4-2-3-10-9-14-6-5-11(10)12;;/h2-6,9,13,15H,7-8H2,1H3;2*1H

SMILES Code: O=S(C1=CC=CC2=C1C=CN=C2)(NCCNC)=O.[H]Cl.[H]Cl


Technical Data

Appearance:
Solid powder

Purity:
>98% (or refer to the Certificate of Analysis)

Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition:
Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility:
Soluble in DMSO

Shelf Life:
>2 years if stored properly

Drug Formulation:
This drug may be formulated in DMSO

Stock Solution Storage:
0 - 4 C for short term (days to weeks), or -20 C for long term (months).

Harmonized System Code:
293490


References

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2: Kraus D, Jäger A, Abuduwali N, Deschner J, Lossdörfer S. Intermittent PTH(1-34) signals through protein kinase A to regulate osteoprotegerin production in human periodontal ligament cells in vitro. Clin Oral Investig. 2012 Apr;16(2):611-8. doi: 10.1007/s00784-011-0541-z. Epub 2011 Mar 29. PubMed PMID: 22186940.

3: De Jonge HR, Tilly BC, Hogema BM, Pfau DJ, Kelley CA, Kelley MH, Melita AM, Morris MT, Viola RM, Forrest JN Jr. cGMP inhibition of type 3 phosphodiesterase is the major mechanism by which C-type natriuretic peptide activates CFTR in the shark rectal gland. Am J Physiol Cell Physiol. 2014 Feb 15;306(4):C343-53. doi: 10.1152/ajpcell.00326.2013. Epub 2013 Nov 20. PubMed PMID: 24259420; PubMed Central PMCID: PMC3919979.

4: Hirai H, Ikeuchi Y, Okada Y. The contribution of PKA to the excitatory mechanism of adenosine in guinea pig superior colliculus slices. Neurosci Lett. 1994 Nov 21;182(1):33-6. PubMed PMID: 7891883.

5: Murakami K, Arai T. [A selective inhibitor of cAMP-dependent protein kinase isolated from an alkalophilic strain of Bacillus species]. Nihon Saikingaku Zasshi. 1989 Nov;44(6):789-96. Japanese. PubMed PMID: 2621791.

6: Yonemaru M, Kasuga I, Kusumoto H, Kiyokawa H, Kuwabara S, Ichinose Y, Toyama K. Protein kinase inhibitor attenuates an increase in endothelial monolayer permeability induced by tumour necrosis factor-alpha. Respirology. 1997 Mar;2(1):63-9. PubMed PMID: 9424406.

7: Santhosh KT, Elkhateeb O, Nolette N, Outbih O, Halayko AJ, Dakshinamurti S. Milrinone attenuates thromboxane receptor-mediated hyperresponsiveness in hypoxic pulmonary arterial myocytes. Br J Pharmacol. 2011 Jul;163(6):1223-36. doi: 10.1111/j.1476-5381.2011.01306.x. PubMed PMID: 21385177; PubMed Central PMCID: PMC3144536.

8: Wolf M, Jäger A, Abuduwali N, Götz W, Lossdörfer S. Continuous PTH modulates alkaline phosphatase activity in human PDL cells via protein kinase C dependent pathways in vitro. Ann Anat. 2013 Oct;195(5):455-60. doi: 10.1016/j.aanat.2013.04.006. Epub 2013 May 13. PubMed PMID: 23742978.

9: Jarvis BW, Qureshi N. Inhibition of lipopolysaccharide-induced transcription factor Sp1 binding by spectrally pure diphosphoryl lipid A from Rhodobacter sphaeroides, protein kinase inhibitor H-8, and dexamethasone. Infect Immun. 1997 May;65(5):1640-3. PubMed PMID: 9125541; PubMed Central PMCID: PMC175189.

10: Gillette MA, Dacheux RF. Protein kinase modulation of GABAA currents in rabbit retinal rod bipolar cells. J Neurophysiol. 1996 Nov;76(5):3070-86. PubMed PMID: 8930256.

11: Xiao Gh, Falkner KC, Xie Y, Lindahl RG, Prough RA. cAMP-dependent negative regulation of rat aldehyde dehydrogenase class 3 gene expression. J Biol Chem. 1997 Feb 7;272(6):3238-45. PubMed PMID: 9013560.

12: Zhang W, Han XY, Wong SM, Takeuchi H. Effects of inhibitors for intracellular signal transduction systems on the inward current produced by GABA in a snail neuron. Gen Pharmacol. 1998 Feb;30(2):221-5. PubMed PMID: 9502177.

13: Maggi CA, Santicioli P, Giuliani S. Protein kinase A inhibitors selectively inhibit the tonic contraction of the guinea pig ureter to high potassium. Gen Pharmacol. 1996 Mar;27(2):341-8. PubMed PMID: 8919654.

14: Selvaraj NG, Prasad R, Goldstein JL, Rao MC. Evidence for the presence of cGMP-dependent protein kinase-II in human distal colon and in T84, the colonic cell line. Biochim Biophys Acta. 2000 Oct 20;1498(1):32-43. PubMed PMID: 11042348.

15: Wang H, Ye Y, Zhu M, Cho C. Increased interleukin-8 expression by cigarette smoke extract in endothelial cells. Environ Toxicol Pharmacol. 2000 Dec;9(1-2):19-23. PubMed PMID: 11137464.

16: Motoyashiki T, Morita T, Ueki H. Involvement of the rapid increase in cAMP content in the vanadate-stimulated release of lipoprotein lipase activity from rat fat pads. Biol Pharm Bull. 1996 Nov;19(11):1412-6. PubMed PMID: 8951155.

17: Poueymirou WT, Schultz RM. Regulation of mouse preimplantation development: inhibition of synthesis of proteins in the two-cell embryo that require transcription by inhibitors of cAMP-dependent protein kinase. Dev Biol. 1989 Jun;133(2):588-99. PubMed PMID: 2543602.

18: Lee YS, Chuong CM. Activation of protein kinase A is a pivotal step involved in both BMP-2- and cyclic AMP-induced chondrogenesis. J Cell Physiol. 1997 Feb;170(2):153-65. PubMed PMID: 9009144.

19: Gwosdow AR, O'Connell NA, Abou-Samra AB. Interleukin-1 increases protein kinase A activity by a cAMP-independent mechanism in AtT-20 cells. Am J Physiol. 1994 Jan;266(1 Pt 1):E79-84. PubMed PMID: 7508195.

20: Chen CC, Chiu KT, Sun YT, Chen WC. Role of the cyclic AMP-protein kinase A pathway in lipopolysaccharide-induced nitric oxide synthase expression in RAW 264.7 macrophages. Involvement of cyclooxygenase-2. J Biol Chem. 1999 Oct 29;274(44):31559-64. PubMed PMID: 10531359.