Chymostatin | CAS#9076-44-2 | protease inhibitor

Chymostatin
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 540074

CAS#: 9076-44-2

Description: Chymostatin is a protease inhibitor used in research models.

Chemical Structure

Chymostatin

CAS# 9076-44-2

Instruction

Theoretical Analysis

MedKoo Cat#: 540074
Name: Chymostatin
CAS#: 9076-44-2
Chemical Formula: C31H41N7O6
Exact Mass: 607.31
Molecular Weight: 607.710
Elemental Analysis: C, 61.27; H, 6.80; N, 16.13; O, 15.80

Price and Availability

Size	Price	Availability
5mg	USD 300	2 Weeks
10mg	USD 500	2 Weeks
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: DB01683; DB 01683; DB-01683; AM-037855; AM 037855; AM037855

IUPAC/Chemical Name: (((R)-1-((R)-2-amino-3,4,5,6-tetrahydropyrimidin-4-yl)-2-(((R)-4-methyl-1-oxo-1-(((R)-1-oxo-3-phenylpropan-2-yl)amino)pentan-2-yl)amino)-2-oxoethyl)carbamoyl)-D-phenylalanine

InChi Key: MRXDGVXSWIXTQL-ZFXZZAOISA-N

InChi Code: InChI=1S/C31H41N7O6/c1-19(2)15-24(27(40)34-22(18-39)16-20-9-5-3-6-10-20)35-28(41)26(23-13-14-33-30(32)36-23)38-31(44)37-25(29(42)43)17-21-11-7-4-8-12-21/h3-12,18-19,22-26H,13-17H2,1-2H3,(H,34,40)(H,35,41)(H,42,43)(H3,32,33,36)(H2,37,38,44)/t22-,23-,24-,25-,26-/m1/s1

SMILES Code: O=C(O)[C@H](NC(N[C@H]([C@H]1CCN=C(N)N1)C(N[C@H](CC(C)C)C(N[C@H](CC2=CC=CC=C2)C=O)=O)=O)=O)CC3=CC=CC=C3

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Instruction:

View handling instruction

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 607.71 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Roszkowska-Chojecka MM, Walkowska A, Gawryś O, Baranowska I, Kalisz M, Litwiniuk A, Martyńska L, Kompanowska-Jezierska E. Effects of chymostatin, a chymase inhibitor, on blood pressure, plasma and tissue angiotensin II, renal haemodynamics and renal excretion in two models of hypertension in the rat. Exp Physiol. 2015 Sep;100(9):1093-105. doi: 10.1113/EP085325. Epub 2015 Jun 23. PubMed PMID: 26011164.

2: Yoshida T, Lepp Z, Kadota Y, Satoh Y, Itoh K, Chuman H. Comparative analysis of binding energy of chymostatin with human cathepsin A and its homologous proteins by molecular orbital calculation. J Chem Inf Model. 2006 Sep-Oct;46(5):2093-103. PubMed PMID: 16995740.

3: Ahamed A, Singh A, Ward OP. Chymostatin can combine with pepstatin to eliminate extracellular protease activity in cultures of Aspergillus niger NRRL-3. J Ind Microbiol Biotechnol. 2007 Feb;34(2):165-9. Epub 2006 Dec 20. PubMed PMID: 17177024.

4: Messdaghi D, Dietz K. Characterization of an extracellular chymostatin-sensitive serine protease preferentially expressed in young plant tissues. Biochim Biophys Acta. 2000 Jul 14;1480(1-2):107-16. PubMed PMID: 10899613.

5: Moniwa N, Varagic J, Simington SW, Ahmad S, Nagata S, Voncannon JL, Ferrario CM. Primacy of angiotensin converting enzyme in angiotensin-(1-12) metabolism. Am J Physiol Heart Circ Physiol. 2013 Sep 1;305(5):H644-50. doi: 10.1152/ajpheart.00210.2013. Epub 2013 Jun 28. PubMed PMID: 23812388; PubMed Central PMCID: PMC3761328.

6: Maguire J, Davenport AP. Alternative pathway to endothelin-converting enzyme for the synthesis of endothelin in human blood vessels. J Cardiovasc Pharmacol. 2004 Nov;44 Suppl 1:S27-9. PubMed PMID: 15838298.

7: Stein RL, Strimpler AM. Slow-binding inhibition of chymotrypsin and cathepsin G by the peptide aldehyde chymostatin. Biochemistry. 1987 May 5;26(9):2611-5. PubMed PMID: 3607037.

8: Libby P, Goldberg AL. Effects of chymostatin and other proteinase inhibitors on protein breakdown and proteolytic activities in muscle. Biochem J. 1980 Apr 15;188(1):213-20. PubMed PMID: 7406880; PubMed Central PMCID: PMC1162558.

9: Place GA, Chetland J, Galpin IJ, Beynon RJ. The effect of analogues of chymostatin on lysosomal and non-lysosomal components of protein degradation in isolated hepatocytes. Biochim Biophys Acta. 1987 Aug 13;925(2):185-93. PubMed PMID: 2887208.

10: Hoffmann T, Martin MM. CE-ESI-MS/MS as a rapid screening tool for the comparison of protein-ligand interactions. Electrophoresis. 2010 Apr;31(7):1248-55. doi: 10.1002/elps.200900585. PubMed PMID: 20196030.

11: Simon O, Bohley P, Bergner H, Münchmeyer R. [Action of proteinase inhibitors. 4. Effect of short-term application of chymostatin on nitrogen metabolism in the digestive tract and protein metabolism in tissue]. Arch Tierernahr. 1984 May-Jun;34(5-6):341-51. German. PubMed PMID: 6466093.

12: Becari C, Sivieri DO Jr, Santos CF, Moysés MK, Oliveira EB, Salgado MC. Role of elastase-2 as an angiotensin II-forming enzyme in rat carotid artery. J Cardiovasc Pharmacol. 2005 Oct;46(4):498-504. PubMed PMID: 16160604.

13: Santos-Neto MS, Carrithers SL, Carvalho AF, Monteiro HS, Greenberg RN, Forte LR, Fonteles MC. Guanylin and its lysine-containing analogue in the isolated perfused rat kidney: interaction with chymotrypsin inhibitor. Pharmacol Toxicol. 2003 Mar;92(3):114-20. PubMed PMID: 12753425.

14: Feinstein G, Malemud CJ, Janoff A. The inhibition of human leucocyte elastase and chymotrypsin-like protease by elastatinal and chymostatin. Biochim Biophys Acta. 1976 May 13;429(3):925-32. PubMed PMID: 1083745.

15: Li J, Lu H, Plante E, Meléndez GC, Levick SP, Janicki JS. Stem cell factor is responsible for the rapid response in mature mast cell density in the acutely stressed heart. J Mol Cell Cardiol. 2012 Oct;53(4):469-74. doi: 10.1016/j.yjmcc.2012.07.011. Epub 2012 Jul 29. PubMed PMID: 22850284; PubMed Central PMCID: PMC3438908.

16: Benoit C, Gu Y, Zhang Y, Alexander JS, Wang Y. Contractility of placental vascular smooth muscle cells in response to stimuli produced by the placenta: roles of ACE vs. non-ACE and AT1 vs. AT2 in placental vessel cells. Placenta. 2008 Jun;29(6):503-9. doi: 10.1016/j.placenta.2008.03.002. Epub 2008 Apr 15. PubMed PMID: 18417209; PubMed Central PMCID: PMC2556283.

17: Komatsu K, Matsui A, Satoh S. Beneficial effect of chymostatin on dystrophic mice. Exp Neurol. 1986 Jul;93(1):110-6. PubMed PMID: 3732454.

18: Chang KO, Takahashi D, Prakash O, Kim Y. Characterization and inhibition of norovirus proteases of genogroups I and II using a fluorescence resonance energy transfer assay. Virology. 2012 Feb 20;423(2):125-33. doi: 10.1016/j.virol.2011.12.002. Epub 2011 Dec 24. PubMed PMID: 22200497; PubMed Central PMCID: PMC3259199.

19: Tomkinson NP, Galpin IJ, Beynon RJ. Synthetic analogues of chymostatin. Inhibition of chymotrypsin and Streptomyces griseus proteinase A. Biochem J. 1992 Sep 1;286 ( Pt 2):475-80. PubMed PMID: 1530579; PubMed Central PMCID: PMC1132922.

20: Tokunaga M, Kawamura A, Yonekyu S, Kishida M, Hishinuma F. Secretion of mouse alpha-amylase from fission yeast Schizosaccharomyces pombe: presence of chymostatin-sensitive protease activity in the culture medium. Yeast. 1993 Apr;9(4):379-87. PubMed PMID: 8511968.

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