EDHB

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 525594

CAS#: 3943-89-3

Description: EDHB is a pan-PHD inhibitor.

Chemical Structure

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EDHB
CAS# 3943-89-3
Instruction

Theoretical Analysis

MedKoo Cat#: 525594
Name: EDHB
CAS#: 3943-89-3
Chemical Formula: C18H18O7
Exact Mass: 346.11
Molecular Weight: 346.335
Elemental Analysis: C, 62.42; H, 5.24; O, 32.34

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

Request quote for custom synthesis

Synonym: EDHB

IUPAC/Chemical Name: Ethyl-3,4-dihydroxybenzoate; Ethyl protocatechuate

InChi Key: IEWPWVSCXPNIRW-UHFFFAOYSA-N

InChi Code: InChI=1S/C18H18O7/c1-3-11-12(6-7-13(19)16(11)21)18(23)25-15-8-5-10(9-14(15)20)17(22)24-4-2/h5-9,19-21H,3-4H2,1-2H3

SMILES Code: O=C(OCC)C1=CC=C(OC(C2=CC=C(O)C(O)=C2CC)=O)C(O)=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 346.34 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Harnoss JM, Platzer LK, Burhenne J, Radhakrishnan P, Cai J, Strowitzki MJ, Weiss J, Ritter AS, Mollenhauer M, Schmidt T, Ulrich A, Haefeli WE, Schneider M. Prolyl Hydroxylase Inhibition Enhances Liver Regeneration Without Induction of Tumor Growth. Ann Surg. 2017 Apr;265(4):782-791. doi: 10.1097/SLA.0000000000001696. PubMed PMID: 28266966.

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5: Harnoss JM, Platzer LK, Burhenne J, Radhakrishnan P, Cai J, Strowitzki MJ, Weiss J, Ritter AS, Mollenhauer M, Schmidt T, Ulrich A, Haefeli WE, Schneider M. Prolyl Hydroxylase Inhibition Enhances Liver Regeneration Without Induction of Tumor Growth. Ann Surg. 2016 Mar 8. [Epub ahead of print] PubMed PMID: 26967635.

6: Li W, Hou G, Zhou D, Lou X, Xu Y, Liu S, Zhao X. The roles of AKR1C1 and AKR1C2 in ethyl-3,4-dihydroxybenzoate induced esophageal squamous cell carcinoma cell death. Oncotarget. 2016 Apr 19;7(16):21542-55. doi: 10.18632/oncotarget.7775. PubMed PMID: 26934124; PubMed Central PMCID: PMC5008304.

7: Singh DP, Nimker C, Paliwal P, Bansal A. Ethyl 3,4-dihydroxybenzoate (EDHB): a prolyl hydroxylase inhibitor attenuates acute hypobaric hypoxia mediated vascular leakage in brain. J Physiol Sci. 2016 Jul;66(4):315-26. doi: 10.1007/s12576-015-0429-9. Epub 2015 Dec 9. PubMed PMID: 26649730.

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10: Miklasińska M, Kępa M, Wojtyczka RD, Idzik D, Zdebik A, Orlewska K, Wąsik TJ. Antibacterial Activity of Protocatechuic Acid Ethyl Ester on Staphylococcus aureus Clinical Strains Alone and in Combination with Antistaphylococcal Drugs. Molecules. 2015 Jul 23;20(8):13536-49. doi: 10.3390/molecules200813536. PubMed PMID: 26213908.

11: Nimker C, Kaur G, Revo A, Chaudhary P, Bansal A. Ethyl 3,4-dihydroxy benzoate, a unique preconditioning agent for alleviating hypoxia-mediated oxidative damage in L6 myoblasts cells. J Physiol Sci. 2015 Jan;65(1):77-87. doi: 10.1007/s12576-014-0348-1. Epub 2014 Nov 8. PubMed PMID: 25381174.

12: Fan L, Li J, Yu Z, Dang X, Wang K. Hypoxia-inducible factor prolyl hydroxylase inhibitor prevents steroid-associated osteonecrosis of the femoral head in rabbits by promoting angiogenesis and inhibiting apoptosis. PLoS One. 2014 Sep 22;9(9):e107774. doi: 10.1371/journal.pone.0107774. eCollection 2014. PubMed PMID: 25244080; PubMed Central PMCID: PMC4171501.

13: Kwon BJ, Lee MH, Koo MA, Han JJ, Park JC. Ethyl-3,4-dihydroxybenzoate with a dual function of induction of osteogenic differentiation and inhibition of osteoclast differentiation for bone tissue engineering. Tissue Eng Part A. 2014 Nov;20(21-22):2975-84. doi: 10.1089/ten.TEA.2013.0567. Epub 2014 Jun 23. PubMed PMID: 24784993; PubMed Central PMCID: PMC4229862.

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18: Li B, Takeda K, Yokoyama S, Shibahara S. A prolyl-hydroxylase inhibitor, ethyl-3,4-dihydroxybenzoate, induces haem oxygenase-1 expression in human cells through a mechanism independent of hypoxia-inducible factor-1alpha. J Biochem. 2008 Nov;144(5):643-54. doi: 10.1093/jb/mvn115. Epub 2008 Sep 17. PubMed PMID: 18799519.

19: Zhong Z, Ramshesh VK, Rehman H, Currin RT, Sridharan V, Theruvath TP, Kim I, Wright GL, Lemasters JJ. Activation of the oxygen-sensing signal cascade prevents mitochondrial injury after mouse liver ischemia-reperfusion. Am J Physiol Gastrointest Liver Physiol. 2008 Oct;295(4):G823-32. doi: 10.1152/ajpgi.90287.2008. Epub 2008 Sep 4. PubMed PMID: 18772364; PubMed Central PMCID: PMC2575910.

20: Kant R, Diwan V, Jaggi AS, Singh N, Singh D. Remote renal preconditioning-induced cardioprotection: a key role of hypoxia inducible factor-prolyl 4-hydroxylases. Mol Cell Biochem. 2008 May;312(1-2):25-31. doi: 10.1007/s11010-008-9717-5. Epub 2008 Feb 14. PubMed PMID: 18273560.