JT010
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MedKoo CAT#: 530362

CAS#: 917562-33-5

Description: JT010 is a potent and TRPA1-selective channel activator via covalent modification of TRPA1 active site Cys621. JT010 opens the TRPA1 channel by covalently and site-selectively binding to Cys621 (EC50 = 0.65 nM). The results suggest that a single modification of Cys621 is sufficient to open the TRPA1 channel. The TRPA1-selective probe described herein might be useful for further mechanistic studies of TRPA1 activation.


Chemical Structure

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JT010
CAS# 917562-33-5

Theoretical Analysis

MedKoo Cat#: 530362
Name: JT010
CAS#: 917562-33-5
Chemical Formula: C16H19ClN2O3S
Exact Mass: 354.08
Molecular Weight: 354.849
Elemental Analysis: C, 54.16; H, 5.40; Cl, 9.99; N, 7.89; O, 13.53; S, 9.03

Price and Availability

Size Price Availability Quantity
50mg USD 550 2 Weeks
100mg USD 950 2 Weeks
200mg USD 1650 2 Weeks
500mg USD 2650 2 Weeks
1g USD 3850 2 Weeks
2g USD 6450 2 Weeks
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Synonym: JT010; JT-010; JT 010.

IUPAC/Chemical Name: 2-Chloro-N-(4-(4-methoxyphenyl)thiazol-2-yl)-N-(3-methoxypropyl)-acetamide

InChi Key: KZMAWJRXKGLWGS-UHFFFAOYSA-N

InChi Code: InChI=1S/C16H19ClN2O3S/c1-21-9-3-8-19(15(20)10-17)16-18-14(11-23-16)12-4-6-13(22-2)7-5-12/h4-7,11H,3,8-10H2,1-2H3

SMILES Code: O=C(N(C1=NC(C2=CC=C(OC)C=C2)=CS1)CCCOC)CCl

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Biological target: JT010 is a potent agonist of TRPA1 with an EC50 of 0.65 nM.
In vitro activity: Moreover, JT010, a TRPA1 agonist, increased the intracellular calcium concentration and extracellular signal-regulated kinase 1/2 phosphorylation, and up-regulated alkaline phosphatase mRNA in human dental pulp cells. Reference: Am J Pathol. 2020 Dec;190(12):2417-2426. https://pubmed.ncbi.nlm.nih.gov/32919979/
In vivo activity: TBD

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 69.0 194.30
Ethanol 26.9 75.72

Preparing Stock Solutions

The following data is based on the product molecular weight 354.85 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Tazawa K, Kawashima N, Kuramoto M, Noda S, Fujii M, Nara K, Hashimoto K, Okiji T. Transient Receptor Potential Ankyrin 1 Is Up-Regulated in Response to Lipopolysaccharide via P38/Mitogen-Activated Protein Kinase in Dental Pulp Cells and Promotes Mineralization. Am J Pathol. 2020 Dec;190(12):2417-2426. doi: 10.1016/j.ajpath.2020.08.016. Epub 2020 Sep 11. PMID: 32919979. 2. Suo Y, Wang Z, Zubcevic L, Hsu AL, He Q, Borgnia MJ, Ji RR, Lee SY. Structural Insights into Electrophile Irritant Sensing by the Human TRPA1 Channel. Neuron. 2020 Mar 4;105(5):882-894.e5. doi: 10.1016/j.neuron.2019.11.023. Epub 2019 Dec 19. PMID: 31866091; PMCID: PMC7205012.
In vitro protocol: 1. Tazawa K, Kawashima N, Kuramoto M, Noda S, Fujii M, Nara K, Hashimoto K, Okiji T. Transient Receptor Potential Ankyrin 1 Is Up-Regulated in Response to Lipopolysaccharide via P38/Mitogen-Activated Protein Kinase in Dental Pulp Cells and Promotes Mineralization. Am J Pathol. 2020 Dec;190(12):2417-2426. doi: 10.1016/j.ajpath.2020.08.016. Epub 2020 Sep 11. PMID: 32919979. 2. Suo Y, Wang Z, Zubcevic L, Hsu AL, He Q, Borgnia MJ, Ji RR, Lee SY. Structural Insights into Electrophile Irritant Sensing by the Human TRPA1 Channel. Neuron. 2020 Mar 4;105(5):882-894.e5. doi: 10.1016/j.neuron.2019.11.023. Epub 2019 Dec 19. PMID: 31866091; PMCID: PMC7205012.
In vivo protocol: TBD

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1: Takaya J, Mio K, Shiraishi T, Kurokawa T, Otsuka S, Mori Y, Uesugi M. A Potent
and Site-Selective Agonist of TRPA1. J Am Chem Soc. 2015 Dec 23;137(50):15859-64.
doi: 10.1021/jacs.5b10162. PubMed PMID: 26630251.